Background: Development and progression of cancer is accompanied by different morphological and functional changes of cells. One of the most important changes is the expression and activity of enzymes in the cellular fatty acids metabolism that reflects in cell membrane lipid composition and increases fluidity of cancer cell membrane. The Ellipin, prepared from bovine liver, is a newly developed anticancer agent containing several important fatty acids.Goal: To investigate effects of Ellipin on hepatic cancer cell function such as proliferation, migration and adherent activity and apoptosis of cancer cell lines in vitro.Materials and Methods: This study was conducted in the Laboratory of Molecular Biology, Institute of Biology, MAS. The Ellipin was developed in the Drug Research Institute of Monos group. HepG2, HCC, 23132/87, MDCK cells lines and the primary liver cancer cells (PCC) were used for proliferation assay. Only HepG2 cell line was used for MTT, Migration, Spreading and Apoptosis assays.Results: The results of proliferation assay showed that the ellipin decreased the proliferation activity of HepG2 and PCC cells depending on concentrations; in 50μg/ml 2-3 times, 250μg/ml fully stopped cells divisions. The Ellipin reduced mitochondrial reeducates enzyme activity of HepG2 cells depending on its concentrations. For example, in 50μg/ml ellipin concentration case, the number of alive cells decreased 2 times. The migration of HepG2 cells treated with 100μg/ml ellipin was decreased by 22.3% compared to the control cells. Also the number of adhered cells was reduced by Ellipin treatment. After 50μg/ml, 100μg/ml, 250μg/ml ellipin treatment, the number of apoptic cells were 14,6%, 45,6%, 100% of initial culture cells, respectively.Conclusions: Our results showed that the Ellipin suppresses HepG2 cancer cell proliferation and decreases migration and spreading activities and also inducts the cell apoptosis.

Atherogenic dyslipidemia comprises a triad of increased blood concentrations of small, dense low density lipoprotein (LDL) particles, decreased high-density lipoprotein (HDL) particles, and increased triglycerides. A typical feature of obesity, the metabolic syndrome, atherogenic dyslipidemia has emerged as an important risk factor for cardiovascular disease. We have determined levels of serum lipid profiles in 1861 older people who lives 5 regions in Mongolia. The concentrations of total cholesterol, triglycerides and high density lipoprotein cholesterol (HDL-C) were measured using a biochemical reagents by biochemical fully automated analyzer. The levels of LDL-C were calculated by the Friedewald equation. Overall prevalence of dyslipidemia was 4.3% in men and 3.0% in women. Logistic regression showed that Odds ratio of the atherogenic dyslipidemia was OR=1.3, p=0.001 (CI 95% 0.93-2.47) for body mass index, OR=1.6, p=0.02 (CI 95% 1.0-2.88) for waist circumference, OR=1.76, p=0.03 (CI 95% 1.12-3.54) for waist hip ratio. Odds ratio of the atherogenic dyslipidemia was OR=0.98, p=0.001 (CI 95% 0.34-1.05) for gender and OR=1.0 p=0.001 (CI 95% 0.65-1.03) for age. Overall, 3.3% of older people had atherogenic dyslipidemia and 4.3% of men and 3.0% of women had atherogenic dyslipidemia. An increase of physical parameters are getting a risk factor of atherogenic dyslipidemia.

Background: Anemia is still being a population’s challenging issue regardless of high development of countries around the world. According to a study regarding prevalence and etiology of anemia conducted in 187 countries around the world, the anemia rate is 23 176 per 100 000 population. In accordance with the “5th National Nutrition Survey” study conducted in our country in 2017, one of every five women (21.4%), aged 15-49 years, 16.2% of reproductive age women and 3.0% of men are anemic. The current study was conducted due to it is still essential to study and identify the etiology of anemia, determine its prevalence, plan appropriate intervention, and organize future preventive measures, depending on the socio-economic conditions, location, diet, and customs of the Mongolians. Aim: To identify anemia among the adult population, determine the type and severity of anemia. Materials and Methods: The cross sectional study was conducted during between May 2022 and Sep 2023 and adults aged above 18 years were included. Moreover, we identified anemia cases based on the laboratory test results and determined the anemia severity grade. Statistical analysis was performed by using SPSS software. Results: Among the participants, 6.7% of them, 9.9% of females and 2.8% of males are anemic. Prevalence of mild, moderate and severe anemia is 66.7%, 30% and 3.3%, respectively. While the prevalence of anemia among reproductive age women is higher, the prevalence of anemia increases with age. The anemia prevalence in Ulaanbaatar region, Western region, Khangai mountainous region, Central region and Eastern region is 583 (25.6%); 171 (7.5%); 343 (15.1%); 921 (40.4%); and 261 (11.4%), respectively. By regional location, the prevalence is high in the Central region, with varying prevalence in other regions. Conclusion Anemia was detected in 6.7% of the total study population, 9.9% of females, and 2.8% of males were anemic. Of those with anemia, 66.7% had mild anemia, 30% had moderate anemia, and 3.3% had severe anemia. Moderate and severe anemia were more common in females than in males.

Монгол улсад элэгний хавдрын өвчлөл ихсэх хандлагатай байгаа бөгөөд эмийн эмчилгээнд гаж нөлөө багатай ургамлын гаралтай эм хэрэглэх хандлага нэмэгдэж энэ чиглэлийн судалгаа эрчимтэй өрнөж байна. Бидний судалгааны ажлын зорилго нь Байгалийн гүүн хөх Scutellaria baicalensis (Georgi) ургамлын хандны элэгний хавдрын эсэд үзүүлэх нөлөөг in vitro орчинд судлан тогтоох явдал юм. Байгалийн гүүн хөх Scutellaria baicalensis (Georgi) ургамлаас гаргаж авсан этанолын ханд элэгний анхдагч өмөнгийн эсэд дарангуйлах нөлөөтэй байх магадлалтай. Судалгаанд элэгний хавдрын анхдагч эсийн өсгөвөр-PCC, нохойн бөөрний MDCK шугаман эс болон Байгалийн гүүн хөх Scutellaria baicalensis (Georgi) ургамлын газрын дээд хэсгийн этанолон ханд, Scutellaria baicalensis (Georgi) ургамлын үндэсний этанолон хандаас ялгасан Skullcap бэлдмэлийг ашиглав. Байгалийн гүүн хөх Scutellaria baicalensis (Georgi) ургамлын ханд болон Skullcap бэлдмэл нь эсийн геномын ДНХ-д нөлөөлж задрал, гэмтэл үзүүлээгүй. Харин нэгж эсээс ялгасан ДНХ-ийн концентраци харилцан адилгүй байгаа нь хандаар үйлчилсэний дараа амüд үлдсэн эсүүдийн тоотой шууд хамааралтай байх магадлалтай. Байгалийн гүүн хөх Scutellaria baicalensis (Georgi) ургамлын ханд болон Skullcap бэлдмэл нь шууд ДНХ-д нөлөөлөхгүй, өөр механизмаар эсийг үхэлд хүргэж байна. Бидний судалгаагаар Байгалийн гүүн хөх Scutellaria baicalensis (Georgi) ургамлын ханд 100 µg/ml бэлдмэл эсийн Bcl-2 генийн экспрессийг идэвхжүүлж 6%-иар байв. Skullcap бэлдмэл (100 µg/ml) 4,8 %, Байгалийн гүүн хөх Scutellaria baicalensis (Georgi) ургамлын ханд 13,2%-иар ZFYVE1 генийн экспрессийг дарангуйлж байна. 100 µg/ml концентраци бүхий бэлдмэл болон хандаар үйлчлэхэд тус бүр GAPDH генийн экспресс 13,4% ба 18,9%-иар тус тус дарангуйлагдсан. 100 µg/ml бэлдмэл болон хандаар GPR175 генийн экспрессийг 34,1% ба 41,5%-иар тус бүр дарангуйлж байв. Бидний судалгааны дүнд 100 µg/ml хандаар үйлчлэхэд GPC3 генийн экспресс илэрсэнгүй. Судалгаагаар эсийн апоптозийн процессийг гүйцэтгэгч CASP3 генийг олшруулах туршилтыг 3 давталттай явуулсан ч уг ген илэрсэнгүй.