1.The Restorative Effect of Gallic Acid on the Experimental Sciatic Nerve Damage Model
Gokhan GURKAN ; Mumin Alper ERDOGAN ; Gurkan YIGITTURK ; Oytun ERBAS
Journal of Korean Neurosurgical Society 2021;64(6):873-881
Objective:
: Peripheral nerve injuries occur mostly as a result of mechanical trauma. Due to the microvascular deterioration in peripheral nerve damage, it becomes challenging to remove free oxygen radicals. Gallic acid is a powerful antioxidant with anti-inflammatory effects and a free radical scavenger. The purpose of the study is to show that gallic acid contributes to the restorative effect in mechanical nerve damage, considering its antioxidant and anti-inflammatory effects.
Methods:
: Thirty male Sprague Dawley albino mature rats were included in the study. Ten of them constituted the control group, 10 out of 20 rats for which sciatic nerve damage was caused, constituted the saline group, and 10 formed the gallic acid group. Post-treatment motor functions, histological, immunohistochemical, and biochemical parameters of the rats were evaluated.
Results:
: Compared to the surgery+saline group, lower compound muscle action potential (CMAP) latency, higher CMAP amplitude, and higher inclined plane test values were found in the surgery+gallic acid group. Similarly, a higher nerve growth factor (NGF) percentage, a higher number of axons, and a lower percentage of fibrosis scores were observed in the surgery+gallic acid group. Finally, lower tissue malondialdehyde (MDA) and higher heat shock protein-70 (HSP-70) values were determined in the surgery+gallic acid group.
Conclusion
: Gallic acid positively affects peripheral nerve injury healing due to its anti-inflammatory and antioxidant effects. It has been thought that gallic acid can be used as a supportive treatment in peripheral nerve damage.
2.Granulocyte-colony stimulating factor decreases the extent of ovarian damage caused by cisplatin in an experimental rat model.
Ali AKDEMIR ; Burak ZEYBEK ; Levent AKMAN ; Ahment Mete ERGENOGLU ; Ahmet Ozgur YENIEL ; Oytun ERBAS ; Altug YAVASOGLU ; Mustafa Cosan TEREK ; Dilek TASKIRAN
Journal of Gynecologic Oncology 2014;25(4):328-333
OBJECTIVE: To investigate whether granulocyte-colony stimulating factor (G-CSF) can decrease the extent of ovarian follicle loss caused by cisplatin treatment. METHODS: Twenty-one adult female Sprague-Dawley rats were used. Fourteen rats were administered 2 mg/kg/day cisplatin by intraperitoneal injection twice per week for five weeks (total of 20 mg/kg). Half of the rats (n=7) were treated with 1 mL/kg/day physiological saline, and the other half (n=7) were treated with 100 microg/kg/day G-CSF. The remaining rats (n=7, control group) received no therapy. The animals were then euthanized, and both ovaries were obtained from all animals, fixed in 10% formalin, and stored at 4degrees C for paraffin sectioning. Blood samples were collected by cardiac puncture and stored at -30degrees C for hormone assays. RESULTS: All follicle counts (primordial, primary, secondary, and tertiary) and serum anti-Mullerian hormone levels were significantly increased in the cisplatin+G-CSF group compared to the cisplatin+physiological saline group. CONCLUSION: G-CSF was beneficial in decreasing the severity of follicle loss in an experimental rat model of cisplatin chemotherapy.
Animals
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Anti-Mullerian Hormone/blood
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Antineoplastic Agents/*toxicity
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Biological Markers/blood
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Cisplatin/*toxicity
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Disease Models, Animal
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Drug Evaluation, Preclinical/methods
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Female
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Fertility Preservation/methods
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Granulocyte Colony-Stimulating Factor/*therapeutic use
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Ovarian Follicle/drug effects/pathology
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Primary Ovarian Insufficiency/blood/chemically induced/pathology/*prevention & control
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Rats, Sprague-Dawley