Humans ; Oxidative Stress ; drug effects ; Oxidoreductases ; metabolism ; physiology

OBJECTIVETo assess the effect of oxidative stress in development of acute high altitude response (AHAR) during the process of strong physical work at high altitude and its change after return to lower altitude.

METHODSNinety-six officers and soldiers of rapid entering into high altitude (3 700 m) with strong physical work were analyzed, all subjects were male, aged 18-35 years. According to the symptomatic scores of AHAR were divided into 3 groups: severe AHAR (group A, n = 24), mild AHAR (group B, n = 47) and without AHAR (group C, n = 25). Levels in serum 8-iso prostaglandinF2alpha(8-iso-PGF2alpha), superoxide dismutase (SOD) and malonaldehyde (MDA) were measured at higher altitude stayed 50 d and after return to lower altitude (1 500 m) 12 h and 15 d, and 50 healthy volunteers (group D) at 1 500 m altitude served as controll.

RESULTSLevels of serum 8-iso-PGF2alpha and MDA [(9.53 +/- 0.47) microg/L, (8.91 +/- 0.39) micromol/L] were significantly higher in group A than those in group B [(8.34 +/- 0.42) microg/L, (7.31 +/- 0.32) micromol/L] , group C [(7.02 +/- 0.48) microg/L, (6.41 +/- 0.23) micromol/L] and group D [(5.13 +/- 0.56) microg/L, (5.48 +/- 0.33) micromol/L], (all P < 0.01), and serum SOD [(52.08 +/- 3.44) micro/ml] was significantly lower in group A than that in group B [62.27 +/- 2.54) micro/ml], group C [(71.99 +/- 3.35) micro/ml] and group D [(80.78 +/- 3.44) micro/ ml] (all P < 0.01), there were significant differences between group B and C, C and D (all P < 0.01). At altitude 3 700 m 50 d, AHAR scores was positively correlated with serum 8-iso-PGF2alpha and MDA (all P < 0.01), negatively correlated with SOD (P < 0.01). Serum 8-iso-PGF2alpha and MDA were negatively correlated with SOD (all P < 0.01). Levels of serum 8-iso-PGF2alpha and MDA were significantly higher at altitude of 3 700 m 50 d than those at altitude of 1 500 m 12 h,15 d in group D (all P < 0.01), and serum SOD was significantly lower than that at 1 500 m 12 h,15 d in group D (all P < 0.01), there were significantly difference between at 1 500 m 12 h and 15 d (all P < 0.01), there were no difference between at 15 d in group D (all P > 0.05).

CONCLUSIONThe more serious of oxidative stress and oxidative/antioxidative imbalance, the more serious of AHAR, oxidative stress and oxidative/antioxidative imbalance may be involved in the development of AHAR. The changes were obviously improved after return to lower altitude 12 h, and recovered to normal after 15 d.

Adolescent ; Adult ; Altitude ; Altitude Sickness ; physiopathology ; Humans ; Male ; Oxidative Stress ; physiology ; Physical Exertion ; physiology ; Young Adult

Local oxidative stress and inflammatory response are the essential procedures in wound repair, which determine the progress, prognosis, and quality of wound repair. Reactive oxygen species is one of the important indexes reflecting oxidative stress and inflammatory response of body, which is considered as a promising target to be regulated in wound inflammation. Recently, with the rapid development of nanomedicine, our research group and other research groups have successfully developed various diagnosis and treatment reagents for reactive oxygen species through interdisciplinary integration, to monitor and regulate reactive oxygen species in wounds in real time, and to finally achieve the goal of improving the speed and quality of wound repair, thus providing a new strategy and direction for the diagnosis and treatment of local inflammatory response in wounds. This article summarizes reactive oxygen species as the regulatory target of local inflammatory response in wounds, in situ monitoring and precise regulation of reactive oxygen species.
Humans ; Reactive Oxygen Species ; Wound Healing/physiology* ; Oxidative Stress/physiology* ; Inflammation

AIMTo explore the influence of cold strss on DNA oxidative damage of lung in chicken.

METHODSTook 15-day-old healthy chicks as the experimental object, carried on the cold stress (12 +/- 1 degrees C) to process. Detected the change of the MDA content, SOD and GSH-Px activity of the lung, and performed KCl-SDS precipitation method and fluorescence detection method to identify the influence of cold strss on DNA-protein crosslinks (DPC) and DNA-DNA crosslinks (DDC) of lung cell in different time.

RESULTSThe results were as follow: with the time lapsing during acute cold stress, MDA content gradually increased, the SOD and GSH-Px activity of the lung increased compared with their control group at each stress time point, and the lung cell DPC and DDC coefficient were all gradually increased with the time lapsing.

CONCLUSIONCold stress could bring about destruction in the lung tissue oxidation-antioxidant balance, and causes the oxidation damage of DNA.

Animals ; Animals, Newborn ; Chickens ; Cold Temperature ; DNA Damage ; Lung ; pathology ; Male ; Oxidative Stress ; physiology ; Stress, Physiological ; physiology

The physiological roles of the glutathione(GSH)/glutathione peroxidase(GPx) system in protecting microbial cells against oxidative stress were reviewed. In eukaryotic model microbe Saccharomyces cerevisiae,this system is obligatory in maintaining the redox balance and defending the oxidative stress. However, the GSH/GPx system only conditionally exists in prokaryotes. Namely,for those prokaryote bacteria containing glutathione reductase and GPx, e.g. Haemophilus influenzae and Lactococcus lactis, by taking up GSH, they might develop a conditional GSH-dependent GPx reduction system, which conferred cells a stronger resistance against oxidative challenge.
Glutathione ; metabolism ; physiology ; Glutathione Peroxidase ; metabolism ; physiology ; Glutathione Reductase ; physiology ; Haemophilus influenzae ; physiology ; Lactococcus lactis ; physiology ; Oxidative Stress ; physiology ; Saccharomyces cerevisiae ; enzymology ; physiology

Animals ; Humans ; Models, Biological ; Oxidative Stress ; genetics ; physiology ; Phosphorylation ; Protein Kinase C ; genetics ; metabolism ; physiology ; Signal Transduction ; genetics ; physiology

OBJECTIVETo investigate the time order of autophagy and apoptosis in human U251 cells injury after H2O2 treatment.

METHODS4 groups in this study were set up, normal control group, 1 mmol/L H2O2 (6 h,12 h, 24 h) group. The viability of U251 cells treated with H2O2 was measured by MTT assay. Cell apoptotic ratio was determined by flow cytometry analysis. Autophagic vacuoles were stained with monodansylcadaverine. The protein level of Beclin 1 and cytosolic cyt c were assayed by using Western blot.

RESULTSCompared with the control group, cell viability decreased significantly under 1 mmol/L H2O2 treatment in time-dependent way. Autophagic vacuoles and the expression of autophagic protein Beclin 1 increased at 6 h, but cell apoptotic ratio and cytosolic cyt c protein did not change obviously, cell apoptotic ratio and cytosolic cyt c protein level increased at 12 h and 24 h (P < 0.05).

CONCLUSIONOxidative stress induced autophagy and apoptosis in U251 glioma cells, and autophagy eventuated ahead of apoptosis.

Apoptosis ; physiology ; Autophagy ; physiology ; Brain Neoplasms ; pathology ; Cell Line, Tumor ; Glioma ; pathology ; Humans ; Oxidative Stress ; physiology ; Time Factors

Animals ; Antioxidants ; physiology ; Dehydroepiandrosterone ; physiology ; Lipid Metabolism ; physiology ; Male ; Oxidative Stress ; drug effects ; Rats ; Rats, Sprague-Dawley

Animals ; Atrial Fibrillation ; physiopathology ; Atrial Remodeling ; physiology ; Calcium ; metabolism ; Humans ; Inflammation ; etiology ; Ion Channels ; physiology ; MicroRNAs ; physiology ; Oxidative Stress

Animals ; Carotid Body ; physiology ; Endothelins ; physiology ; Humans ; Hypertension ; etiology ; Hypoxia ; complications ; Inflammation ; complications ; Oxidative Stress ; Sympathetic Nervous System ; physiology