1.Effects of Kimchi Extract and Temperature on Embryostasis of Ascaris suum Eggs.
Jin Sung KIM ; Dae Sung OH ; Kyu Sung AHN ; Sung Shik SHIN
The Korean Journal of Parasitology 2012;50(1):83-87
To determine the effects of kimchi extracts at different temperatures on larval development, Ascaris suum eggs were mixed with soluble part of 7 different brands of commercially available kimchi and preserved at either 5degrees C or 25degrees C for up to 60 days. A. suum eggs incubated at 25degrees C showed marked differences in larval development between kimchi extract and control group. While all eggs in the control group completed embryonation by day 21, only 30% of the eggs in the kimchi extract group became embryonated by day 36 and about 25% never became larvated even at day 60. At 5degrees C, however, none of the eggs showed larval development regardless of the incubation period or type of mixture group. To determine the survival rate of A. suum eggs that showed no embryonation after being preserved at 5degrees C, eggs preserved in kimchi extracts for 14, 28, and 60 at 5degrees C were re-incubated at 25degrees C for 3 weeks in distilled water. While all eggs in the control group became larvated, eggs in the kimchi extract group showed differences in their embryonation rates by the incubation period; 87.4 % and 41.7% of the eggs became embryonated after being refrigerated for 14 days and 28 days, respectively. When refrigerated for 60 days, however, no eggs mixed in kimchi extract showed larval development. Our results indicate that embryogenesis of A. suum eggs in kimchi extract was affected by duration of refrigeration, and that all eggs stopped larval development completely in kimchi kept at 5degrees C for up to 60 days.
Animals
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Ascaris suum/*drug effects/embryology
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Brassica/*chemistry
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Ovum/*drug effects/growth & development
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Plant Extracts/*pharmacology
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Raphanus/*chemistry
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Temperature
2.In vitro anthelminthic efficacy of Dichrocephala integrifolia (Asteraceae) extracts on the gastro-intestinal nematode parasite of mice: Heligmosomoides bakeri (Nematoda, Heligmosomatidae).
Poné J WABO ; V K PAYNE ; Tayo Gertrude MBOGNING ; Marie Claire KOMTANGI ; Jeannette YONDO ; Alidou M NGANGOUT ; Mbida MPOAME ; Bilong C F BILONG
Asian Pacific Journal of Tropical Biomedicine 2013;3(2):100-104
OBJECTIVETo evaluate the ovicidal and larvicidal activities of aqueous and ethanolic extracts of leaves of Dichrocephala integrifolia (D. integrifolia) against the eggs (fresh and embryonnated), the first and second larval stages of Heligmosomoides bakeri. In order to verify if this medicinal plant possesses active compounds capable of inhibiting the embryonation and hatching of eggs or to induce the mortality of larvae (L1 and L2).
METHODSdried extracts were diluted in distilled FIV water to obtain five different concentrations: 625, 1,250, 2,500, 3,750 and 5,000 µg/mL. Fresh eggs obtained from artificially infected mice feces were exposed to these different concentrations for 48 h. Time of contact for embryonated eggs was 6 h while L1 and L2 larvae were exposed for 24 h. Distilled water (placebo) and 1.5% DMSO were used as negative controls.
RESULTSDistilled water, and 1.5% DMSO had no effect on embryonation, hatching and larval survival. Aqueous extracts of D. integrifolia showed a weak activity against all stages of the parasite at all concentrations tested. On the contrary, the ethanolic extract of D. integrifolia inhibited the embryonation of 87.5% of fresh eggs, the hatching of 81.1% of embryonated eggs and induced the mortality of 98.1% and 98% of L1 and L2 larvae respectively at 5,000 µg/mL.
CONCLUSIONSThe results of the present study indicate that the ethanolic extracts of D. integrifolia contained compounds with ovicidal and larvicidal properties. In spite of these results, in vivo tests, studies on toxicity and mechanism of action of active compounds are also needed to validate the utilisation of this medicinal plant by population of Dschang-Cameroon to treat gastro-intestinal parasites.
Animals ; Antinematodal Agents ; pharmacology ; therapeutic use ; Asteraceae ; chemistry ; Dose-Response Relationship, Drug ; Heligmosomatoidea ; drug effects ; growth & development ; Larva ; drug effects ; Mice ; parasitology ; Ovum ; drug effects ; Plant Extracts ; pharmacology ; therapeutic use ; Plant Leaves ; chemistry ; Rodent Diseases ; drug therapy ; Strongylida Infections ; drug therapy ; veterinary