Adult ; Animals ; Female ; Frameshift Mutation/genetics* ; Genes, sry ; Gonadal Dysgenesis, 46,XY/genetics* ; Humans ; Menarche ; Ovary/pathology* ; Oviducts/pathology* ; Sex-Determining Region Y Protein/genetics*

OBJECTIVETo study the effects of Yifuning soft gelatin capsules (YFN) on pathology and biomechanical indexes of ovariectomied female rats.

METHODSixty female Sprague-Dawley rats without pregnancy and delivery were used,50 of them were ovariectomized and randomly divided into 5 groups: ovariectomy(OVX), OVX with diethylstilbestrol tables (DT), OVX with YFN (high dose,middle dose and low dose), the others were sham-operated group. The rats began to take the rats drugs in the fifth week after the operation. After 12 weeks of treat ment the rats were killed, and the bones were collected to inspect. The pathology of bone was observed with the HE stain and the area of trabecular was detected. The electronic testing device was used to detect the biomechanical properties.

RESULTThe results indicated that the rat model of postmenopausal osteoporosis (PMO) was a success. Compared with the model group, after application of the drugs, the observation of HE showed different repairing around trabecular. The biomechanical parameters also increased significantly.

CONCLUSIONYFN can effectively prevent the loss of bone in OVX rats and strengthen bone quality, and has a good effect on PMO oostmenopausal osteoporosis.

Animals ; Biomechanical Phenomena ; Bone Density ; Capsules ; Curcuma ; chemistry ; Drug Combinations ; Female ; Femur ; physiopathology ; Lumbar Vertebrae ; physiopathology ; Materia Medica ; administration & dosage ; isolation & purification ; pharmacology ; Oils, Volatile ; administration & dosage ; isolation & purification ; pharmacology ; Osteoporosis ; pathology ; physiopathology ; Ovariectomy ; Oviducts ; chemistry ; Plants, Medicinal ; chemistry ; Random Allocation ; Ranidae ; Rats ; Rats, Sprague-Dawley

OBJECTIVETo investigate the effects of the Yifuning soft gelatin capsules(YFN)on estrogen receptor (ER) and nitric oxide (NO) levels in ovariectomized rats.

METHODFifty female mature sprague-dawley rats were randomly divided into 5 groups: normal control; model control; diethylstilbestrol tablets (DT); YFN (high dose and low dose). After 4 weeks' treatment, the serum E2 levels were detected by radioimmunoassay. The estrogen receptor (ER) levels of uterus and artery were detected with method of radioligand binding assay of receptor (RBAR). The uterus pathologic changes were investigated with light microscope. NO and NOS levels of the artery and the uterus index were detected too.

RESULTYFN can obviously improve serum E2, increase index and ER of uterus (P < 0.01 or P < 0.05), and ameliorate the uterus' pathologic changes in OVX rats. It also can increase the artery' ER, NO and NOS levels.

CONCLUSIONYFN can cure the climacteric syndrome and prevent the cardiovascular disease after post-menopause.

Animals ; Aorta ; metabolism ; Capsules ; Curcuma ; chemistry ; Drug Combinations ; Drugs, Chinese Herbal ; administration & dosage ; isolation & purification ; pharmacology ; Estradiol ; blood ; Female ; Materia Medica ; administration & dosage ; isolation & purification ; pharmacology ; Nitric Oxide ; metabolism ; Nitric Oxide Synthase ; metabolism ; Ovariectomy ; Oviducts ; chemistry ; Plants, Medicinal ; chemistry ; Rana temporaria ; Random Allocation ; Rats ; Rats, Sprague-Dawley ; Receptors, Estrogen ; metabolism ; Uterus ; metabolism ; pathology