0.05).Conclusions:1.The acute rise in FFAs level for a short term can inhibit ACTH and cortisol secretion in healthy male adults,but with prolonged FFA elevation this inhibition last less than 24 hours.2.The acute FFAs rise has no influence on ACTH or cortisol circadian rhythm.

In the present study, Mongolian gerbil was used to make delayed neuronal damage (IND) animal model. In 6 different brain regions, 5 kinds of biochemical indexes in the aspect of free radical metabolism were measured at 7 time phases during ischemia and reperfusion. The results showed that, after transient cerebral ischemia and during reperfusion, some unique changes appeared tn hippocampal CA1 sector, which were characterized by the persistent increase of free radical content and persistent decrease of the Mn-SOD activity. The fluctuating changes could be seen in Cu, Zn-SOD activity and lipid peroxide content. There were no significant changes in the brain regions other than CA1 sector. It is concluded that the disturbance of free radical metabolism play a key role in the occur. fence of DND. It was also observed that Aniracetam, Helicid and Nieardipine had some control effects to a certatn extent on the free radical disturbance above in hippocampal CA1 sector of DND animals.

Resiliency is individual's capability to cover difficulties and make a good adaptation.This research reviews the researches on resiliency abroad.It summarizes the practical researches of resiliency from 3 aspects: ① The concept of resiliency;②The structure and intension of resiliency;③The measurement of resiliency.On this basis,it raises some questions in resiliency researches and puts forward the future direction.

Objective To evaluate apelin level in patients with obesity and different glucose tolerances, investigate relevance of apelin with body mass index(BMI), blood lipid and blood glucose. Methods According to different glucose results of oral glucose tolerance test, 120 subjects were divided into three groups: normal glucose tolerance(NGT),impaired glucose tolerance(IGT) and type 2 diabetes(T2DM). All of the subjects were also divided into obese and non-obese subgroups based on their BMI(≥25kg/m~2 or ＜25kg/m~2 ). Plasma apelin levels were compared among groups of NGT, IGT and T2DM. Fasting plasma glucose(FPG)and 2h postprandial plasma glucose (2hPG) after OGTT, lipid profile and BMI were measured. Results Both fasting and 2h postprandial plasma apelin had significant differences in these groups (P<0.05,or P<0.01). The overweight and obese subgroups in the IGT group and diabetes group had higher apelin levels than their counterpart subgroups in the NGT group. BMI, FPO, systolic blood pressure(SBP) were independent influencing factors for apelin (P<0.05 ,or P<0.01). Conclusions Plasma apelin level is related to obesity and type 2 diabetes.

OBJECTIVETo assess the quality of reporting by randomized controlled trial (RCT) related to dental implants in China during 2000 to 2012 by using the revised Jadad scale and consolidated standards of reporting trials (CONSORT) (2010) statement.

METHODSThe following electronic databases were searched: Chinese Biomedical Literature Database, Database for Chinese Technical Periodicals, China National Knowledge Infrastructure, PubMed, and EMBASE. A total of 19 journals of stomatology in China were also searched manually. The qualities of RCT with dental implant published between 2000 and 2012 were assessed using CONSORT (2010) statement and revised Jadad scale.

RESULTSTwenty-eight RCTs related to dental implants were identified. The quality of reporting in 28 articles was low. The mean revised Jadad score was 1.29 ± 0.71 and the CONSORT (2010) score was 9.75 ± 3.60.

CONCLUSIONThe methodological qualities of the included studies on dental implants are generally low, and reporting quality remain unsatisfactory.

China ; Dental Implants ; Humans ; Publishing ; Randomized Controlled Trials as Topic

OBJECTIVETo explore the features of various mediastinal lymphadenopathies using computed tomography perfusion (CTP).

METHODSCTP parameters (CTPs) of the selected mediastinal nodes from 59 patients with pathology-proven malignant lymph nodes and of those from 29 patients with clinically diagnosed or pathology-proven inflammatory lymphadenopathies were collected. Patients were divided into subgroups by etiology and phase of primary disease, including different pathological malignant nodes and diverse inflammatory nodes. CTPs were defined as blood flow (BF), blood volume (BV), mean transit time (MTT), permeability (PMB), and time to peak (TTP). Differences of CTPs were compared between malignant and benign nodes, and among subgroups, respectively.

RESULTSIn the mediastinum, no significant differences of CTPs were found between malignant and benign groups (all P>0.05), the same for subgroups of malignant nodes (all P>0.05). Acute lymphadenitis had higher BF and BV than chronic inflammatory, lymphoid tuberculosis, sarcoidosis and malignant nodes. The BF of malignant nodes was markedly slower than that of acute lymphadenitis (P=0.01), but faster than chronic inflammatory nodes (P=0.04) and sarcoidosis (P=0.03), with no significant difference compared with lymphoid tuberculosis. Pneumonia-complicated lymphoid tuberculosis showed the longest MTT while sarcoidosis displayed the shortest MTT, and inflammatory nodes, lymphoid tuberculosis without complicated pneumonia and malignant nodes had moderate MTT.

CONCLUSIONCTPs show promising potential in distinguishing various lymphadenopathies in the mediastinum, but more studies are needed to improve their specificity.

Adult ; Female ; Humans ; Lymph Nodes ; diagnostic imaging ; Lymphatic Diseases ; diagnostic imaging ; Male ; Mediastinal Diseases ; diagnostic imaging ; Perfusion ; Retrospective Studies ; Tomography, X-Ray Computed ; methods

Objective To identify a detailed biological characterization of mesenchymal stem cells (MSCs) isolated from hu‐man umbilical cord(UC) tissue regarding their morphology ,immunophenotype ,purity and proliferative capacity and establish a rea‐sonably cultured and amplified system .Methods After stripping off arteries and veins ,the remaining parts of umbilical cord were cut into 1 mm3 small sections and cultured with DMEM/F12 containing 10% fetal bovine serum .Adhere cells were obtained and the morphology of the cells was observed under inverted phase contrast microscope .The growth curves of them were drawn by CCK‐8 and the cell cycle and surface antigens (CD29 ,CD73 ,CD90 ,CD105 ,CD31 ,CD14 ,CD34 ,CD45 ,CD11b ,HLA‐DR) were detected by flow cytometry .Results Seven to ten days after primary culture ,adhere cells came out of fragments .The MSCs harvested were a high purity and mainly presented as a fibroblast‐like morphology .UC‐MSCs had a strong ability of proliferation through the cell growth curve .The special surface antigens CD29 ,CD73 ,CD90 ,CD105 were positive expression ,while CD31 ,CD14 ,CD34 ,CD45 , CD11b ,HLA‐DR were negative .More than 80% cells of MSCs were found at G0/G1 phase .Conclusion Human UC‐MSCs could be cultured and proliferated in vitro .

ObjectiveTo investigate the diagnosis and treatment of occult thyroid carcinoma(OCT).MethodsRetrospective analysis on the clinical data of 34 cases of OCT was made. ResultsOnly 6 cases were preoperatively diagnosed as OCT. Total resection of the affected lobe and isthmus was performed on 16 cases with neck dissection in 14 cases. Subtotal thyroidectomy was performed on 18 cases. The occult cancer lesion was pathologically confirmed within ipsolateral lobe in all the 34 cases. Neck lymph node metastasis was found in 4 cases and local infiltration in 3 cases. The psammoma bodies in cancer lesion and adjacent normal gland were found in 15 cases. Thirty-one cases were followed up for an average of 9 years. One died of lung metastasis and two more of other unrelated diseases. Conclusion(1) The psammoma bodies in normal thyroid gland usually imply the existence of cancer. (2) Total thyroidectomy of a lobe or subtotal thyroidectomy was the therapy of choice. Local infiltration or neck lymph node metastasis necessitates additional neck dissection.

Objective To explore the combined toxicity of dominant volatile organic compounds(VOCs) and ammonia in indoor air of newly decorated rooms.Methods The gaseous mixture of several main components of VOCs and ammonia was prepared using their pure chemical reagents based on the percentage of mass proportion to each pollutant calculated by the medians of concentrations of formaldehyde,benzene,methylbenzene,dimethylbenzene,ethylbenzene and ammonia obtained from the in situ monitoring data on indoor air of 45 newly decorated apartments.The Kunming mice were exposed to the prepared gaseous mixture at doses of 3.93,8.47,18.24 and 39.35 g/m3 for acute toxicity test,and at doses of 3.935 g/m3(higher dose),1.970 g/m3(middle dose)and 0.393 g/m3(lower dose)for sub-acute toxicity test respectively.Results The LC50 of the prepared gaseous mixture was 26.84 g/m3 for Kunming mice.The sub-acute toxicity test showed that the lower levels of reticulocyte count of female mice in each dose group,the lower level of HCT of female mice dose group,the lower levels of PLT in serum of female and male mice in higher dose group,higher activity of ALT in serum of female mice in higher dose group showed significant differences compared with those of controls (P

1 were also high in DDDs(i.e.high dosage).CONCLUSION: The patients undergoing uterine-incision delivery had a high proportion in using antimicrobial drugs yet the choice of drug kinds was reasonable.However,great attention should be paid to the high dosage of some of the antimicrobial drugs.