Objective To prepare for extraction of liver focal lesion in view of its characteristics of images. Methods A region of interest in the liver region comprising the focal lesion to create a local image was selected firstly, then the threshold to pre-segment the local image was got automatically by using local OTSU in clusters algorithm, at last mark sifting methods to get red of inner holes and outer dissociate areas to get the final contour was used. Results The algorithm can efficiently extract the focal liver lesion. Conclusion The way of extraction of liver focal lesions with local OTSU and zone market screening is rapid and effective.

Objective To observe the long?term efficacy of neoadjuvant chemotherapy followed by intensity?modulated radiation therapy. Methods A retrospective analysis was performed on the data of 183 patients with locally advanced NPC who were hospitalized in our hospital:96 cases in the observation group, 87 cases in the con?trol group. Results There were no significant differences between the observation group and the control group in terms of five?year OS (77.1%vs. 78.2%, P=0.861), DMFS(89.6%vs. 90.8%, P=0.782), RFS (78.1%vs. 77%, P=0.857) and PFS (72.9%vs. 71.3%, P=0.803). Compared with the control group, the observation group did not sig?nificantly improve 5?year OS, DMFS, RFS and PFS in T3?4N0?1 patients and also did not improve 5?year OS, DMFS, RFS and PFS in T1?4N2?3 patients. There were significantly larger numbers of individuals with adverse reactions in the observation group than in the control group. Conclusions Compared with the long?term efficacy in the patients with locally advanced NPC between neoadjuvant chemotherapy followed by intensity?modulated radiation therapy with concurrent chemotherapy and intensity?modulated radiation therapy with concurrent chemotherapy, the progno?sis has no significantly differences and neoadjuvant chemotherapy followed by intensity?modulated radiation therapy with concurrent chemotherapy leads to a larger number of adverse reactions. The action of neoadjuvant chemothera?py followed by intensity?modulated radiation therapy with concurrent chemotherapy in treatment of locally advanced NPC needs more research.

Objective To observe the effect of Chinese medicine to wash, acupressure and Avene cream to moistur-ize for infantile eczema.Methods 100 babies who were diagnosed infantile eczema were chosen and separated to interven-tion group and control group randomly.The control group was treated by Chinese medicine to wash, acupressure and cream to moisturize, and the intervention group was treated by hormone medicine and Avene cream to moisturize.Observe the treat-ment effect after 7 days, 14days and 1 week after cured.Results The severity,effective rate and cure rate of two groups after 7days haven′t the statistical difference(p>0.05), and the severity of intervention group after 14days was lower than that of control group (p<0.05),the effective rate of intervention group after 14days was higher than and that of control group (p<0.05), and the recurrence rate of intervention group was lower than that of control group (p<0.05).Conclusion Combi-ning traditional Chinese and western medicine treatment can promote the treatment effect of infantile eczema , and reduce the recurrence, should be generalization.

Objective Hemophagocytic lymphohistiocytosis (HLH) is a life-threatening condition characterized by excessive inflammation, with a high incidence in children and a death rate of 40%.This study was to analyze the gene expression profile in child-hood HLH and explore the important pathways of childhood HLH using bioinformatic methods . Methods The childhood HLH gene ex-pression profile data GSE26050 were obtained from the Gene Expression Omnibus (GEO) database of the National Center for Biotechnolo-gy Information.Differentially expressed genes were identified with the GEO 2R online analysis tools released recently .The key pathways of the differentially expressed genes were investigated using the Kyoto Encyclopedia of Genes and Genomes ( KEGG) pathway enrichment a-nalysis. Results A total of 184 differentially expressed genes were identified , 126 upregulated and the other 58 downregulated .They were enriched in 3 pathways, including cytokine-cytokine receptor interaction , hematopoietic cell lineage and NOD-like receptor signaling pathways. Conclusion Bioinformatic tools allow the identification of the key genes and pathways associated with the development and progression of childhood HLH and point out the potential directions for researches on the mechanisms of childhood HLH .

Humans ; Hypertension ; metabolism ; pathology ; Transient Receptor Potential Channels ; metabolism ; physiology

AIM:To investigate the mechanism of endothelium-derived microparticles(EMP)-induced endothelial dysfunction and the role of superoxide anion(O-?2) in EMP-induced endothelial dysfunction.METHODS:EMP were isolated from human umbilical vein endothelial cells stimulated with plasminogen activated inhibitor-1.(1) Cultured bovine aortic endothelial cells(BAEC) were divided into 3 groups and pretreated with nothing in group 1,EMP(1?108/L) in group 2,EMP(1?108/L) + L-nitroarginiemethylester(L-NAME,1 mmol/L) in group 3 for 30 min and A23187(5 ?mol/L) stimulated O-?2 generation was determined by superoxide dismutase(SOD)-inhibitable ferricytochrome C reduction.(2) Facialis arteries(60-150 microns) were isolated from C57BL/6 mice and divided into 4 groups.The vessels were pretreated with nothing in group 1,EMP(1?108/L) in group 2,EMP(1?108/L) + SOD(2?105 U/L) in group 3,EMP(1?108/L) + polyethylene glycolated-SOD(PEG-SOD,2?105 U/L) in group 4 for 10 min and acetylcholine(ACH)-induced vasodilation was measured.RESULTS:(1) EMP significantly increased O-?2 generation in BAEC culture,which was prevented about 50% by pretreating the BAEC with L-NAME.(2) EMP significantly impaired ACH-induced vasodilation.SOD could not restore EMP-impaired ACH-induced vasodilation and PEG-SOD showed partial restoration of vasodilation.CONCLUSION:These data indicate that at least some EMP-induced endothelial dysfunction occurs by inducing intracellular O-?2 generation.It may provide a theoretical evidences in finding a multiple treatment including removal of O-?2 in the future.

Objective To compare the diagnostic value of sound velocity(SV) and real-time ultrasonic elastography in differentiating benign or malignant of breast lesions.Methods 75 patients with 99 lesions were examined with the zone speed index technique and real-time ultrasonic elastography respectively.Then the SVs of the lesions were calculated and elastography of the lesions were scored with 5-scoring method.The ROC curves were constructed with histology as the golden standard.Results There was significant difference between the SVs of benign and malignant breast lesions(P =0.0001).1561 m/s was the best cufoff point.And the areas under the curves (AUC) of SV and real-time ultrasonic elastography were 0.842 and 0.968,respectively.There was significant difference between the two methods of AUC (P =0.023).The sensitivity,specificity and accuracy for SV were 81.5％,91.7％,88.9％,respectively.There was no significant difference between sensitivity,specificity and accuracy of the two methods(P =1.0,P =0.125,P =0.146).Conclusions Both SV method and real-time ultrasonic elastography are helpful in the evaluation of benign or malignant of breast lesions.

Functional targets are the objects that Chinese herbal medicines act directly upon. If the relationships between the properties of Chinese herbs and their functional targets were analyzed clearly, it would benefit the overall understanding of the holistic mechanisms of Chinese herbal treatments. In this paper, data regarding the properties of Chinese herbs and their functional targets were collected from the 2005 edition of The People's Republic of China Pharmacopoeia. After analyzing and assessing the data, the relationships were defined between the four qi, meridian entry and medicinal functional targets and between the four qi, five flavors and mode of function. Then the relationships between a single herbal medicine and a prescription were analyzed, and the results conformed with the traditional knowledge of Chinese herbal nature and efficacy. This demonstrated that the holistic mechanisms of the properties of Chinese herbs adhere to the findings, which may be beneficial for the development and compatibility of Chinese herbal medicines.

Objective To study the family environment and the behavior of children with emotional disorder(ED ).Methods Thirty children with ED and 30 healthy children were assessed with family environment scale-Chinese version(FES-CV),and Achenbach CBCL.Results The scores on the items of intimateness,emotion,independence,and entertainment among Children with ED had by FES-CV were lower,but the scores on conflicts and control higher than the health controls(P<0?05).The total scores on social abilities as well as the dimensions were all lower than the healthy controls(P<0?01).The total scores on behaviors as well as the dimensions were higher than the controls(P<0?05).Conclusions Children with ED have more behavior problems,which are associated with their poorer family environment.Therefore,the family members should help and support each other to create a healthy environment for children growth.

AIM To examine whether pretreatment with simvastatin protects the heart against free radical injury. METHODS 1 1 diphenyl 2 picryl hydrazyl (DPPH) was used for triggering free radical injury in cardiac tissue. Simvastatin against free radical injury was investigated in a Langendorff fused rat heart. Left ventricular developed pressure (LVDP), maximal velocity of increase of LVP (+d p /d t max ), heart rate (HR) coronary flow (CF) and malondialdehyde (MDA) formation in cardiac tissue were measured. RESULT In the DPPH free radical group, DPPH signficantly decreased LVDP, +d p /d t max , HR was slowed and CF was reduced. The formation of MDA was significantly enhanced. ( P