1.Successful resection of osteosarcoma pulmonary metastasis extending into left side of heart under cardiopulmonary bypass: a case report.
Chinese Medical Journal 2002;115(11):1743-1744
Adult
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Cardiopulmonary Bypass
;
Female
;
Heart Neoplasms
;
surgery
;
Humans
;
Lung Neoplasms
;
secondary
;
Osteosarcoma
;
secondary
;
surgery
2.MR Imaging Findings of a Primary Cardiac Osteosarcoma and Its Bone Metastasis with Histopathologic Correlation.
Sejin AHN ; Jung Ah CHOI ; Jin Haeng CHUNG ; Hocheol CHOI ; Eun Ju CHUN ; Sang Il CHOI ; Heung Sik KANG
Korean Journal of Radiology 2011;12(1):135-139
An osteosarcoma of cardiac origin is extremely rare, and a comprehensive description of MR imaging (MRI) findings of cardiac osteosarcoma and its metastasis in the femur have not been reported in the literature. We present a case of cardiac osteosarcoma in a 47-year-old woman and its metastasis to the femur, focusing on the description of MRI findings of the cardiac and metastatic bony osteosarcoma with a histopathologic correlation.
Female
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Femoral Neoplasms/pathology/*secondary
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Heart Neoplasms/*diagnosis/pathology
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Humans
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*Magnetic Resonance Imaging
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Middle Aged
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Osteosarcoma/*diagnosis/pathology/*secondary
3.Current treatment of primary and metastatic osteosarcoma.
Chinese Journal of Oncology 2012;34(12):881-884
4.Establishment and characterization of cell sublines with high and low metastatic potential derived from human osteosarcoma.
Xiao-bing SHI ; An-ming CHEN ; Xian-hua CAI ; Fen-jing GUO ; Guo-ning LIAO ; Ding MA
Chinese Medical Journal 2005;118(8):687-690
Animals
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Bone Neoplasms
;
pathology
;
Cell Line, Tumor
;
Cell Movement
;
Humans
;
Mice
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Mice, Inbred BALB C
;
Mice, Nude
;
Osteosarcoma
;
pathology
;
secondary
5.Rare occurrence of bilateral breast and peritoneal metastases from osteogenic sarcoma.
Ruoh Shyuan CHAN ; Gnana KUMAR ; Anushya A P VIJAYANANTHAN
Singapore medical journal 2013;54(3):e68-71
Breast metastases are uncommon and typically spread from contralateral breast carcinomas. Breast metastases that spread from extramammary malignancies are even less common, and account for 0.5%-6.6% of all malignant breast disease. As extrapulmonary metastases from osteosarcoma are uncommon, breast metastasis from osteosarcoma is extremely rare. We report a case of breast and peritoneal metastases from a tibial osteosarcoma 18 months after diagnosis, and 9 months after surgery and adjuvant chemotherapy. Computed tomography findings of multiple calcified and noncalcified tumour deposits in the lungs, pleura, peritoneum, chest wall and both breasts are described.
Adolescent
;
Bone Neoplasms
;
diagnosis
;
diagnostic imaging
;
pathology
;
Breast Neoplasms
;
diagnostic imaging
;
secondary
;
Female
;
Humans
;
Neoplasm Metastasis
;
Osteosarcoma
;
diagnosis
;
diagnostic imaging
;
pathology
;
Peritoneal Neoplasms
;
diagnostic imaging
;
secondary
;
Tomography, X-Ray Computed
6.Low-grade extraskeletal osteosarcoma of mediastinum: report of a case.
Hong YU ; Chao-fu WANG ; Xiong-zeng ZHU
Chinese Journal of Pathology 2012;41(11):770-771
Adult
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Chemotherapy, Adjuvant
;
Diagnosis, Differential
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Female
;
Humans
;
Lung Neoplasms
;
secondary
;
Mediastinal Neoplasms
;
diagnostic imaging
;
drug therapy
;
pathology
;
surgery
;
Mediastinum
;
diagnostic imaging
;
pathology
;
Neoplasm Recurrence, Local
;
Osteosarcoma
;
diagnostic imaging
;
drug therapy
;
pathology
;
secondary
;
surgery
;
Tomography, X-Ray Computed
7.Over-expression of Sox4 and β-catenin is associated with a less favorable prognosis of osteosarcoma.
Zheng-Qi BAO ; Chang-chun ZHANG ; Yu-zhou XIAO ; Jian-sheng ZHOU ; Yi-sheng TAO ; Da-min CHAI
Journal of Huazhong University of Science and Technology (Medical Sciences) 2016;36(2):193-199
The purpose of this study was to examine the association of the expression of Sox4 and β-catenin with the prognosis of osteosarcoma. A total of 108 cases of conventional osteosarcoma were involved in this study and 28 cases of osteochondroma served as controls. The expression of Sox4 and β-catenin was detected by using immunohistochemical staining and Western blotting. The results showed that Sox4 and β-catenin were over-expressed in 67 (62.03%) and 62 (57.41%) of 108 osteosarcoma cases, while in only 3 (10.71%) and 5 (17.86%) of 28 controls, respectively (P<0.05 for all). The expression of Sox4 and β-catenin was associated with the distant metastasis, pathological grade and Enneking stage of patients with osteosarcoma (P<0.05 for all). The mean overall survival time and the 5-year-survival rate in osteosarcoma patients with Sox4 and β-catenin over-expressed were significantly reduced as compared with those in Sox4 and β-catenin low-expression group (P<0.05 for all). Cox multifactor regression analysis revealed that the distant metastasis, Enneking stage, and the expression of Sox4 and β-catenin were independent risk factors of patients with osteosarcoma (P<0.05 for all). The findings indicated that overexpression of Sox4 and β-catenin is associated with a poor prognosis of osteosarcoma.
Adolescent
;
Adult
;
Biomarkers, Tumor
;
genetics
;
metabolism
;
Bone Neoplasms
;
metabolism
;
pathology
;
Case-Control Studies
;
Child
;
Female
;
Humans
;
Lung Neoplasms
;
metabolism
;
secondary
;
Male
;
Middle Aged
;
Osteosarcoma
;
metabolism
;
pathology
;
SOXC Transcription Factors
;
genetics
;
metabolism
;
beta Catenin
;
genetics
;
metabolism
8.Prognostic significance of p21(WAF1) expression in osteosarcoma.
Chun-Lin ZHANG ; Wei-Ming LIAO ; Fo-Bao LI ; Bing-Fang ZENG ; Yi-Xin ZENG
Chinese Journal of Pathology 2005;34(8):524-527
OBJECTIVETo investigate the expression of p21(WAF1) gene in human osteosarcoma and their relationships with clinicopathological parameters and prognostic value.
METHODSThe p21(WAF1) gene mRNA and p21 protein expression in 45 osteosarcoma and 10 fibrous dysplasia of bone specimens were analyzed by in situ hybridization and immunohistochemistry respectively.
RESULTS(1) The positive rate of p21 protein expression in osteosarcoma was 17.7% (8/45). (2) The expression rate in high proliferation (4/10) significantly higher than that in low proliferation (4/35) osteosarcoma (chi2 = 4.34, P < 0.05). (3) The positive rate of p21(WAF1) mRNA expression in osteosarcoma was 42.2% (19/45). The expression rate in high proliferation (6/10) significantly higher than that in low proliferation (13/35) osteosarcoma (chi2 = 20.6, P < 0.01). (4) The survival time after operation of the patients with p21(WAF1) mRNA expression were higher than that of the patients with p21(WAF1) mRNA negative expression (P < 0.05).
CONCLUSIONS(1) With the increase in degree of malignancy, the expression of p21(WAF1) mRNA and p21 protein in osteosarcoma tend decrease. (2) The expression of p21(WAF1) mRNA has a definite value in judging prognosis in osteosarcoma.
Adolescent ; Adult ; Bone Neoplasms ; metabolism ; pathology ; Cyclin-Dependent Kinase Inhibitor p21 ; biosynthesis ; genetics ; Female ; Follow-Up Studies ; Gene Expression Regulation, Neoplastic ; Humans ; Lung Neoplasms ; metabolism ; secondary ; Male ; Neoplasm Staging ; Osteosarcoma ; metabolism ; pathology ; Prognosis ; RNA, Messenger ; biosynthesis ; genetics ; Survival Rate
9.CircRNA circTNPO1 promotes the proliferation and metastasis of osteosarcoma by sponging miR-338-3p.
Jian Hong LU ; Xiao Wen HUANG ; Guo Qiang ZHANG ; Yan MA ; Jun Xin CHEN
Chinese Journal of Oncology 2022;44(9):968-974
Objective: To explore the effects of circTNPO1 on the proliferation and metastasis of osteosarcoma (OS) by sponging miR-338-3p. Methods: The expression of circTNPO1 on osteoblasts and multiple OS cell lines were detected by qRT-PCR. CircTNPO1 stable knockdown 143B cell line was constructed by sh-circTNPO1. Cell count kit 8 (CCK-8) assay and wound healing assay were applied to evaluate the proliferation and metastasis of this cell. Luciferase reporter assay was used to explore the binding between circTNPO1 and miR-338-3p. In xenograft tumor model, miR-338-3p inhibitor or its control was injected into the circTNPO1 knockdown tumors. The weight and size of the tumors were evaluated and Ki-67 expression was detected by immunohistochemistry. Results: The RNA expression of circTNPO1 in OS cell lines U2OS, HOS, MG63, 143B, ZOS and ZOSM were 2.73±0.27, 3.18±0.54, 4.33±0.52, 5.75±0.65, 4.50±0.49 and 3.96±0.35, respectively, higher than 1.00±0.09 in hFOB1.19 (P<0.001). CCK-8 assay revealed that after 48 h and 72 h, the absorbance of sh-circTNPO1 #1 was 0.81±0.05 and 1.09±0.06, while sh-circTNPO1 #2 143B cells was 0.84±0.04 and 1.2±0.04, which were sharply reduced compared with the control (1.00±0.06 and 1.49±0.06, P<0.001); after 48 h and 72 h, the absorbance of 143B cells transfected with circTNPO1 #1 and miR-338-3p (0.92±0.06 and 1.32±0.07) were higher than those of cells transfected with sh-circTNPO1 cells and miR NC (0.92±0.06 and 1.32±0.07, P<0.050). Wound healing assay demonstrated that the 24 hour-migration rates of sh-circTNPO1 #1 and sh-circTNPO1 #2 cells were (24.43±2.15)% and (39.70±4.20)% respectively, which were significantly lower than that of the control [(56.51±3.27)%, P<0.010]; the migration rates of sh-circTNPO1 #1+ miR NC and sh-circTNPO1 #1+ miR-338-3p inhibitor were (26.70±2.21)% and (46.10±5.71)%, with a significant difference (P<0.005). In xenograft tumor model, the weight and size of tumors in control, sh-circTNPO1 #1+ miR NC and sh-circTNPO1 #1+ miR-338-3p inhibitor mice were (458.80±158.10) mg, (262.50±82.09) mg, (395.40±137.60) mg and (593.00±228.40) mm(2,) (203.30±144.20) mm(2,) (488.60±208.60) mm(2,) respectively. Compared with control, sh-circTNPO1 tumors were significantly smaller (P<0.01). Injection with miR-338-3p inhibitor significantly reversed both the weight and size of tumors (P<0.05). Conclusion: CircTNPO1 promotes the proliferation and metastasis of OS by sponging miR-338-3p, which could be a new target for OS treatments.
Animals
;
Bone Neoplasms/pathology*
;
Cell Line, Tumor
;
Cell Movement
;
Cell Proliferation/genetics*
;
Gene Expression Regulation, Neoplastic
;
Humans
;
Ki-67 Antigen/metabolism*
;
Mice
;
MicroRNAs/metabolism*
;
Osteosarcoma/secondary*
;
RNA, Circular/metabolism*
;
Sincalide/metabolism*
10.Prosthesis replacement of the proximal humerus after the resection of bone tumors.
Si-Feng SHI ; Yang DONG ; Chun-Lin ZHANG ; Kun BAO ; Xiao-Jun MA
Chinese Journal of Cancer 2010;29(1):121-124
BACKGROUND AND OBJECTIVEAfter chemotherapy was used to treat patients with malignant bone tumors in 1970s, amputation, which was the typical intervention in the 1980s, has been substituted with limb-sparing surgery. This article reported the surgical indications, operative methods, operative effects, and complications of prosthetic replacement of the proximal humerus after the resection of bone tumors.
METHODSFrom April 2004 and December 2008, prosthetic replacement was performed in 18 patients with proximal humerus tumors, including 7 patients with osteosarcoma, 5 patients with chondrosarcoma, 3 patients with giant cell tumor (GCT) of the bone, 1 patient with GCT of the bone combined with an aneurysmal bone cyst, and 1 patient with metastatic bone tumors. Using the Enneking staging system, 7 osteosarcomas and 3 chondrosarcomas were at stage Ib, and 3 GCTs and 2 chondrosarcomas were at stage Ib. The patient with metastatic bone tumors reported severe pain.
RESULTSThe follow-up ranged 5-61 months (mean, 29 months) and showed that 1 patient with osteosarcoma died 19 months after surgery. Local recurrence presented in 1 patient with GCT, 1 patient had inner infection in the area of surgery, and 2 patients had shoulder subluxation after the operation. There was no prosthetic loosening in any patient. The abduction angle of the shoulder was 8 degrees-35 degrees, and circumgyrate angle was 18 degrees-25 degrees, with flexion 35 degrees-90 degrees and extension 25 degrees-42 degrees. According to the functional score developed by the International Society of Limb Salvage, scores ranged between 18 and 29 points, with an average of 24 points.
CONCLUSIONSThe prosthesis replacement for the patients with bone tumors in the proximal humerus is an appropriate procedure with satisfactory therapeutic outcomes; however, many complications should be noted and long-term therapeutic effect needs further investigations.
Adolescent ; Adult ; Arthroplasty, Replacement ; Bone Neoplasms ; diagnostic imaging ; pathology ; surgery ; Chondrosarcoma ; diagnostic imaging ; pathology ; surgery ; Female ; Follow-Up Studies ; Giant Cell Tumor of Bone ; diagnostic imaging ; pathology ; surgery ; Humans ; Humerus ; Lung Neoplasms ; secondary ; Male ; Middle Aged ; Neoplasm Recurrence, Local ; Neoplasm Staging ; Osteosarcoma ; diagnostic imaging ; pathology ; secondary ; surgery ; Prosthesis Implantation ; Radiography ; Range of Motion, Articular ; Young Adult