No abstract available.
Calcium* ; Diet* ; Hypercalciuria* ; Osteocalcin*

No abstract available.
Alkaline Phosphatase* ; Calcium* ; Female ; Humans ; Osteocalcin*

OBJECTIVETo compare the synthetic ability of osteoblasts on the surface of different nano-granule titanium films and investigate the correlation between nanophase titanium films and cellular biocompatibility.

METHODSFour different nano-granule titanium films were produced by direct current magnetron sputtering, at ambient, 100 degrees C, 250 degrees C, 380 degrees C substrate temperature, respectively. Rat osteoblasts were seeded on the surface of four treated groups of titanium film samples and non-treated Ti sample(control group). The production of osteocalcin (OC) in all five groups were detected by using double antibody sandwich enzyme-linked immunosorbent assay.

RESULTSThe production of OC increased gradually from day 7 to day 14 in all groups. In the control group, it showed significant differences with other five groups on day 7. On day 14, the production of OC in 100 degrees C group was the highest, and it showed significant differences with 380 degrees C, control group and blank group. In 250 degrees C group, the production of OC also showed significant differences with 380 degrees C, control group and blank group (P < 0.05).

CONCLUSIONTitanium with nano-modified surface had good biocompatibility and different nano-granule titanium films could affect the synthesis of osteoblasts.

OBJECTIVETo determine the role of 1,25(OH)2D3 on the secondary dentin formation and mineralization of the mice.

METHODSThe differences of the mandible mineralization between the wild-type and 1-alpha-hydroxylase gene knockout mice at 6 weeks old were assessed by hematoxylin-eosin (HE) staining, immunohistochemistry, alkaline phosphatase (ALP) histochemistry staining.

RESULTSThe ratio of caries were increased significantly, while the secondary dentin was reduced significantly, the deposition of type I collagen and osteocalcin on the secondary dentin of occlusion surface was decreased significantly, but the deposition of the Biglycan on the dentin was increased significantly, the active of ALP on the odontoblasts were reduced significantly in 1-alpha-hydroxylase gene knockout mice compared to that in the wild-type littermates.

CONCLUSION1,25(OH)2D3 deficiency lead to a defect in the secondary dentin formation and mineralization and caries of the mice.

No abstract available.
Cytosine Deaminase* ; Cytosine* ; Genetic Therapy* ; Osteocalcin* ; Prostate* ; Prostatic Neoplasms*

BACKGROUND: In contrast with bone formation markers, most of available indices of bone resorption are urine markers and show relatively high degree of variability. The serum resorption assay has therefore been developed. We evaluated serum bone-derived degradation products of type I collagen C-telopeptide (s-CTX) and serum osteocalcin by Elecsys 2010 (Hitachi Boehringer Mannheim, Tokyo, Japan). METHODS: For 18 healthy controls, 15 osteopenic and 7 osteoporotic patients samples, serum CTX and serum osteocalcin were measured by Elecsys 2010 using -CrossLaps/serum (Roche Diagnostic Corp., Indianapolis, USA) kit and N-MID Osteocalcin (Roche Diagnostic Corp. kit, respectively. DPD by Immulite (Diagnostic Products Corp., LA, USA) using Pyrilinks-D(TM) (Diagnostic Products Corp.) kit and serum osteocalcin for correlation by Gamma counter (Hewlett Packard, Meriden, USA) using ELSA-OSTEO (CIS, Cedex, France) kit were measured. RESULTS: The within-run and between-run coefficient of variation (CV) values of s-CTX were 6.41% and 6% in low concentrations and 3.84% and 7% in high concentrations, respectively. The within-run and between-run CV values of serum osteocalcin were 2.21% and 6% in low concentrations and 1.25% and 3% in high concentrations, respectively. The dilution recovery of s-CTX and serum osteocalcin was 100-169% (mean, 134%) and 80-138% (mean, 104%), respectively. S-CTX and DPD (R=0.369, P=0.019), and serum osteocalcin by Elecsys 2010 and RIA (R=0.889, P<0.001) showed positive correlations, respectively. CONCLUSTIONS: S-CTX and serum osteocalcin by Elecsys 2010 exhibits good analytical performance and correlate with DPD and serum osteocalcin by RIA, respectively. Therefore, these may replace DPD and serum osteocalcin by RIA and can be used for bone resorption and formation markers, respectively.
Bone Resorption ; Collagen Type I ; Humans ; Osteocalcin* ; Osteogenesis

The purpose of this study was to evaluate the relationship between the osteoporotic condition and periodontal condition in postmenopausal women with periodontitis. Forty three female postmenopausal patients with no systemic disease were grouped into 3 groups by their periodontal conditions; 12 mild periodontitis, 11 moderate periodontitis and 20 advanced periodontitis. From each patient, age of menopause was taken, alkaline phosphatase(ALP) and osteocalcin (OC) in blood and deoxypyridinoline (DPD) in urine were measured. Bone mineral density (BMD) of lumbar spine (L2-L4) was measured by dual energy X-ray absorptiometry. Periodontal and osteoporotic parameters were compared among the groups and correlation coefficient between them was evaluated. The blood ALP and OC levels were similar among the groups with different periodontal condition, whereas the urine DPD level and BMD were significantly lower in advanced periodontitis group than the other groups(p<0.01). Probing depth was negatively related with BMD (r=-0.5, p<0.01) and positively related with patient age and the duration of menopause (r= 0.32 and 0.35 respectively, p<0.05). Clinical attachment loss was negatively related with BMD (r=-0.66, p<0.01), and positively related with urine DPD (r= 0.37, p<0.05). These results showed that postmenopausal women with advanced periodontitis had significantly decreased bone mineral density and suggests that decreased bone mineral density in postmenopausal women could be associated with periodontal tissue breakdown.
Absorptiometry, Photon ; Bone Density ; Female ; Humans ; Menopause ; Osteocalcin ; Periodontitis* ; Spine

PURPOSE: We investigated the usefulness of urinary pyridinoline (uPyr) and deoxypyridinoline (uDpyr) and serum osteocalcin as markers of bone metastasis, particularly focussing on quantitative correlation between the degree of bone metastasis and the level of biochemical markers. MATERIALS AND METHODS: By using ELISA method we measured the levels of uPyr, uDpyr, and osteocalcin in 100 cancer patients of whom 58 patients had bone metastasis, 42 had no bone metastasis, and 44 control subjects. RESULTS: There was a significant difference in uPyr level between the patients with bone metastasis and the patients without bone metastasis or control group (mean+/-SD, 70.26+/-43.11 vs 38.93+/-21.48 or 25.13+/-8.81 nM/mM Creatinine, p<0.05). And uDpyr level showed more significant elevation in the patients with bone metastasis than in the patients without bone metastasis and in control group (12.63+/-7.51 vs 6.44+/-3.58 and 4.23+/-1.70 nM/mM Creatinine p<0.05). Osteocalcin level showed no significant difference among groups. We could demonstrate a significant quantitative correlation between the extent of bone metastasis and the amount of uPyr (r=0.7482, p<0.001) or uDpyr (r=0.5992, p<0.001). CONCLUSION: uPyr and uDpyr were significantly increased in metastatic bone tumors and quantitatively correlated well with the extent of bone metastasis. Therefore we can use these two markers as an evidence of bone metastasis. Further studies are recommended to decide the usefulness of these markers in the early detection of bone metastasis and in the assessment of response to antiresorptive treatments.
Biomarkers* ; Creatinine ; Enzyme-Linked Immunosorbent Assay ; Humans ; Neoplasm Metastasis ; Osteocalcin

The main objectives of this study were to assess the age related changes of biochemical indices of bone turnover in postmenopausal osteoporotic females, and to assess the correlations of these indices with bone mineral density(BMD) of lumbar spine measured by dual energy X-ray absorptiometry(DEXA). Subgects were 70 osteoporotic women in pre and postmenopausal periods. The results showed that Postmenopausal women had higher level of Osteocalcin(OS) and Deoxypyridinoline(DPYD) with lower level BMD of lumbar spine compared with premenopausal women. Age, height, and weight had significant correlations with BMD of lumbar spine. Also a significant correlation was observed between the OS and DPYD. Pre and postmenopausal osteoporotic women(5, 10, 15 year duration) were similar for the rate of bone turnover. These results indicate that the biochemical indices used in our study are the potential markers to predict an age related change of BMD, as well as bone turnover rate of the lower BMD subjects. The combination of BMD measurement and assessment of the bone turnover rate by measuring biochemical indices would be helpful for the screening and treatment of patients with risks of osteoporosis.
Bone Density ; Female ; Humans ; Mass Screening ; Osteocalcin ; Osteoporosis ; Postmenopause ; Spine

The purpose of this study was to evaluate the correlation between nicotine and the activity of bone forming cell. MG63 osteoblast-like cells were used for this study. Several factors were examined including the proliferation of cell, alkaline phosphatase activity, the formation of osteocalcin and osteoprotegerin, and the synthesis of its mRNA. MG63 osteoblast-like cells were incubated for 1, 2, 3 and 6 days with nicotine added to the culture medium in 1.0 micrometer, 1.0 mM, 2.5 mM, 5.0 mM, 7.5 mM, and 10.0 mM concentrations. The proliferation of MG63 osteoblast-like cells was temporarily activated at the low nicotine concentrations. At high concentrations (>5.0 mM), however, it was suppressed. Alkaline phosphatase activity was suppressed in a dose-dependent manner as the concentration of nicotine increased. Osteocalcin decreased in a dose-dependent manner at high nicotine concentrations of more than 7.5 mM and the same result was show when the osteoblasts were treated with low concentrations for longer than 3 days. There was a difference in the influence of nicotine on the synthesis of osteocalcin mRNA and formation of osteocalcin itself at 1 and 3 days. Generally, osteoprotegrin notably declined in all experimental groups. However, the level of its mRNA inc-reased at high nicotine concentrations of more than 7.5 mM after 3 days and more than 5.0 mM after 6 days.
Alkaline Phosphatase ; Nicotine* ; Osteoblasts ; Osteocalcin* ; Osteoprotegerin* ; RNA, Messenger*