1.Osteocalcin expression in primary bone tumors: in situ hybridization and immunohistochemical study.
Yong Koo PARK ; Moon Ho YANG ; Youn Wha KIM ; Hye Rim PARK
Journal of Korean Medical Science 1995;10(4):263-268
Osteocalcin is one of the most abundant noncollagenous proteins found in adult bone. It is a highly conserved gamma-carboxyglutamic acid-containing protein that is believed to be produced exclusively by osteoblasts. In this study, intracellular and extracellular localization of osteocalcin in osteosarcoma was examined with anti-osteocalcin antibody and in situ hybridization using a synthetic oligonucleotide. Immunohistochemically, osteoblastic osteosarcomas were all positive for osteocalcin. The chondroblastic osteosarcomas were positive on the neoplastic chondrocytes. The five fibroblastic osteosarcomas out of seven were positive for osteocalcin immunostaining over the neoplastic spindle cells. Five cases of osteoblastic osteosarcomas out of seven were positive for osteocalcin in situ hybridization. Two cases of chondroblastic osteosarcomas and three cases of fibroblastic osteosarcomas were positive for in situ demonstration of osteocalcin. The malignant tumor giant cells were positive for osteocalcin immunostaining 83%. They were also positive for in situ hybridization. The benign giant cells in five giant cell tumors and five aneurysmal bone cysts were negative for osteocalcin immunostaining. The benign giant cells in three chondroblastoma and three Paget's disease were positive for osteocalcin. In this study, the osteocalcin in situ hybridization and immunostaining has very important meaning for making differential diagnoses of, especially giant cell rich bone forming tumors.
Base Sequence
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Bone Neoplasms/*chemistry
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Human
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Immunohistochemistry
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In Situ Hybridization
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Molecular Sequence Data
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Osteocalcin/*analysis
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Osteosarcoma/*chemistry
2.Experimental study on the isolated culture of osteocytes and identification of osteoblasts in rats.
Xiao-Kang TANG ; Wan CHENG ; Bing XU ; Hang YING ; Pei-Jian TONG ; Lu-wei XIAO
China Journal of Orthopaedics and Traumatology 2013;26(3):227-231
OBJECTIVETo establish a more stable method to isolate osteocytes in vitro, and then to find the differences with osteoblast biological characteristics.
METHODSOsteocytes and osteoblasts were isolated from the bone tissue of 3-day-old rats using sequential collagenase digestion. The cells were identified through cell morphology after 24 hours later. Alkaline phosphatase (ALP) kit was used to stain the first generation cells by Kaplow-way, the bone gla protein (BGP) of the cells were stained by immunocytochemitry. Measured ALP and computed its activity.
RESULTSOsteocytes and osteoblasts showed obviously differences in cell morphology. Osteocytes were star-shaped or dendrite-shaped within more dendrites, while osteoblasts were spindle-shaped with short dendrites. Osteocytes were negative for ALP, but osteoblasts were positive; Osteocytes were more positive for BGP, and osteoblasts were less positive. The secretion of ALP in osteocytes was lower than that of osteoblasts.
CONCLUSIONOsteocytes can be isolated and cultured in vitro. These characteristics of osteocytes are apparently difference with those of osteoblasts.
Alkaline Phosphatase ; analysis ; Animals ; Cell Separation ; methods ; Osteoblasts ; chemistry ; cytology ; Osteocalcin ; analysis ; Osteocytes ; chemistry ; cytology ; Rats ; Rats, Sprague-Dawley
3.Effects of mechanical stretching force on osteoblast-like function of human periodontal ligament cells in vitro.
Xiaotong LI ; Ding ZHANG ; Minkui FU ; Yanqi YANG
Chinese Journal of Stomatology 2002;37(2):135-138
OBJECTIVETo investigate the osteoblast-like functional characteristics exhibited by human periodontal ligament cells (hPDLCs) under mechanical force.
METHODSHuman PDLCs cultured in vitro were stretched by mechanical force. Radioimmunoassay (RIA) was used to measure the expression of secreting alkaline phosphotase (ALP) and osteocalcin (OCN). The non-secreting ALP, OCN and osteopontin (OPN) in cells were determined by immunohistochemistry.
RESULTSIt exhibited increasing of ALP secreted into conditional media, and in the 24 hour period there were two peaks which appeared at the 2nd and 4th hour and the 24th hour (P < 0.01). While in the late of the 24 hours, expression of OCN in conditional media increased (P < 0.05).
CONCLUSIONMechanical force induces hPDLCs to differentiate into functional osteoblast-like cells and plays a role in bone remodeling.
Alkaline Phosphatase ; metabolism ; Cells, Cultured ; Humans ; Osteocalcin ; analysis ; Osteoclasts ; physiology ; Osteopontin ; Periodontal Ligament ; cytology ; Sialoglycoproteins ; analysis ; Stress, Mechanical
4.A concomitant decrease in cortical and trabecular bone mass in isolated hypogonadotropic hypogonadism and gonadal dysgenesis.
Ki Hyun PARK ; Sang Joon LEE ; Jin Young KIM ; Jeong Yeon KIM ; Sang Wook BAI ; Jae Wook KIM
Yonsei Medical Journal 1999;40(5):444-449
To assess the impact of hypogonadism on bone mineral density, we performed a cross-sectional study of 70 amenorrheic women, comprising 22 cases of gonadal dysgenesis and 48 cases of isolated hypogonadotropic hypogonadism (IHH). Bone mineral density was measured by DEXA at four sites: the femur neck, Ward's triangle, trochanter, and lumbar spine (L2-4). The results were compared to those of a control group consisting of 60 age-matched, normal-cycling women. Bone mineral densities around age 20 were already significantly lower at all four sites in patients with IHH and gonadal dysgenesis when compared with controls, suggesting that these patients failed to achieve peak bone mass during pubertal development. In patients with IHH, the initial BMD around age 18-20 were significantly lower at all four sites and the decrease in bone density continued rapidly during the early twenties up to age 25, and then it slowed markedly thereafter. Bone biochemical marker, ICTP and osteocalcin were significantly negatively correlated with age and remained increased until age 40, which was reminiscent of menopausal bone loss pattern such as high bone turn-over in the early twenties, followed by slow bone loss in the late twenties. In patients with gonadal dysgenesis, bone biochemical marker, ICTP and osteocalcin were also significantly negative correlated with age and remained increased until age 40, but no significant changes in BMD were noted as a function of age, which may be attributed to the small sample size and slow bone loss. These findings suggest that the initiation of prompt and timely therapeutic intervention as early as possible in the menarchal period and throughout the remainder of life, particularly during the period associated with rapid bone loss.
Adolescence
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Adult
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Bone Density*
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Collagen/analysis
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Female
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Gonadal Dysgenesis/therapy
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Gonadal Dysgenesis/metabolism*
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Human
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Hypogonadism/therapy
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Hypogonadism/metabolism*
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Osteocalcin/blood
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Peptides/analysis
;
Puberty
5.The in vivo formation of cementum-like tissue by bovine cementoblasts.
Jian-xia HOU ; Cai-fang CAO ; Huan-xin MENG
Chinese Journal of Stomatology 2003;38(1):70-72
OBJECTIVETo test the bovine cementoblasts (CBs) cementum-forming ability in vivo.
METHODSRoot fragments of newborn bovine freshly extracted mandibular incisor were cultured routinely and 4th-5th passages of CBs were harvested. CBs were then cultured in the medium supplemented with 50 mg/L alpha-ascorbic acid and 10 mmol/l beta-glycerolphosphate to form a thick layer as tissue engineering scaffold for cementum formation. Collagen membrane was used as control scaffold. 2 x 10(6) cells were attached to the CBs-made carrier as well as collagen membrane scaffolds and transplanted subcutaneously into immunodeficient mice. Transplants were harvested at 7th week. Histological sections were stained with HE, alizarin red S and van Kossa methods as well as monoclonal Ab against bovine cementum attachment protein (CAP).
RESULTSCBs-made scaffold supported more cementum-like tissue (CLT) formation than collagen-made scaffold. The CLT formed on CBs scaffold was partly calcified with embedded cells. Uncalcified cementoid-like material could be seen on the surface and was encircled by cubical CB-like cells. The CLT was also positive to CAP and van Kossa staining.
CONCLUSIONSThese results suggest that the bovine CBs can form cementum-like tissue. The cell-made carrier is a better scaffold than collagen membrane.
Alkaline Phosphatase ; analysis ; Animals ; Bone Transplantation ; methods ; Cattle ; Cell Adhesion Molecules ; analysis ; Cells, Cultured ; Dental Cementum ; chemistry ; cytology ; transplantation ; Immunohistochemistry ; Integrin-Binding Sialoprotein ; Male ; Mice ; Mice, Nude ; Osteocalcin ; analysis ; Osteonectin ; analysis ; Sialoglycoproteins ; analysis ; Tissue Engineering ; methods ; Transplantation, Heterologous
6.Changes of osteocalcin and IGF-I during bone lengthening.
Bing WANG ; Yi-yun LIU ; Jie-bai ZHENG ; Shi-xiang YUAN ; Guo-xun CHEN
Chinese Journal of Traumatology 2005;8(3):151-155
OBJECTIVETo examine the changes of osteocalcin and insulin-like growth factor I (IGF-I) during bone lengthening, and to clarify the mechanism of bone healing.
METHODSThirty-two shepherd dogs were divided into five groups randomly. Their tibiae were lengthened by Ilvzarov's external fixator at the rate of 1 mm/day. The lengthening area was the experimental side and the opposite side was the control. Samples were obtained on the 2nd, 4th, 6th, 8th, 12th weekend respectively. The samples were defatted, dried, powdered, centrifuged and measured by radioimmunoassay.
RESULTSThe osteocalcin concentration increased at the subsequent periods, but it was significantly lower in the experimental side than that of the control side, P<0.05 and the IGF-I concentration was not significantly lowered.
CONCLUSIONSDifferent noncollagenous bone growth factors may be different at different stage.
Analysis of Variance ; Animals ; Biomarkers ; analysis ; Bone Lengthening ; methods ; Disease Models, Animal ; Dogs ; Female ; Insulin-Like Growth Factor I ; analysis ; metabolism ; Male ; Osteocalcin ; analysis ; metabolism ; Probability ; Radioimmunoassay ; Random Allocation ; Sensitivity and Specificity ; Statistics, Nonparametric ; Tibia ; surgery
7.Parameters of Calcium Metabolism Fluctuated during Initiation or Changing of Antipsychotic Drugs.
Dragan R MILOVANOVIC ; Marijana STANOJEVIC PIRKOVIC ; Snezana ZIVANCEVIC SIMONOVIC ; Milovan MATOVIC ; Slavica DJUKIC DEJANOVIC ; Slobodan M JANKOVIC ; Dragan RAVANIC ; Milan PETRONIJEVIC ; Dragana IGNJATOVIC RISTIC ; Violeta MLADENOVIC ; Mirjana JOVANOVIC ; Sandra NIKOLIC LABOVIC ; Marina PAJOVIC ; Danijela DJOKOVIC ; Dusan PETROVIC ; Vladimir JANJIC
Psychiatry Investigation 2016;13(1):89-101
OBJECTIVE: Serum parameters of calcium homeostasis were measured based on previously published evidence linking osteoporotic fractures and/or bone/mineral loss with antipsychotics. METHODS: Prospective, four-week, time-series trial was conducted and study population consisted of patients of both genders, aged 35-85 years, admitted within the routine practice, with acute psychotic symptoms, to whom an antipsychotic drug was either introduced or substituted. Serial measurements of serum calcium, phosphorous, magnesium, 25(OH)D, parathyroid hormone, calcitonin, osteocalcin and C-telopeptide were made from patient venous blood samples. RESULTS: Calcium serum concentrations significantly decreased from baseline to the fourth week (2.42+/-0.12 vs. 2.33+/-0.16 mmol/L, p=0.022, n=25). The mean of all calcemia changes from the baseline was -2.6+/-5.7% (-24.1 to 7.7) with more decreases than increases (78 vs. 49, p=0.010) and more patents having negative sum of calcemia changes from baseline (n=28) than positive ones (n=10) (p=0.004). There were simultaneous falls of calcium and magnesium from baseline (63/15 vs. 23/26, p<0.001; OR=4.75, 95% CI 2.14-10.51), phosphorous (45/33 vs. 9/40, p<0.001; 6.06, 2.59-14.20) and 25(OH)D concentrations (57/21 vs. 13/35, p<0.001; 7.31, 3.25-16.42), respectively. Calcemia positively correlated with magnesemia, phosphatemia and 25(OH)D values. Parathyroid hormone and C-telopeptide showed only subtle oscillations of their absolute concentrations or changes from baseline; calcitonin and osteocalcin did not change. Adjustment of final calcemia trend (depletion/accumulation) for relevant risk factors, generally, did not change the results. CONCLUSION: In patients with psychotic disorders and several risks for bone metabolism disturbances antipsychotic treatment was associated with the decrease of calcemia and changes in levels of the associated ions.
Antipsychotic Agents*
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Blood Chemical Analysis
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Bone and Bones
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Calcitonin
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Calcium*
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Homeostasis
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Humans
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Ions
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Magnesium
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Metabolism*
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Minerals
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Osteocalcin
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Osteoporotic Fractures
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Parathyroid Hormone
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Prospective Studies
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Psychotic Disorders
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Risk Factors
8.The relationship between osteoprotegerin (OPG)-receptor activator of NF-kB ligand (RANKL) gene polymorphisms and change in bone mineral density after hormone therapy in postmenopausal Korean women.
Jung Gu KIM ; Seung Yup KU ; Byung Chul JEE ; Chang Suk SUH ; Seok Hyun KIM ; Young Min CHOI ; Shin Yong MOON
Korean Journal of Obstetrics and Gynecology 2007;50(6):901-911
OBJECTIVE: To investigate the relationship of polymorphisms in osteoprotegerin (OPG)-receptor activator of NF-kB ligand (RANKL) gene to changes in circulating OPG and soluble RANKL (sRANKL) levels and in bone mineral density (BMD) after hormone therapy (HT) in postmenopausal Korean women. METHODS: The OPG T245G, and G1181C polymorphisms and RANKL rs2277438 A/G polymorphism were analyzed by polymerase chain reaction-restriction fragment length polymorphsim (PCR-RFLP) or direct DNA sequencing in 236 postmenopausal Korean women receiving sequential HT for 1 year. Serum OPG, sRANKL, bone alkaline phosphatase, CrossLaps (CTX), osteocalcin, clacitonin, parathyroid hormone, calicum, and phophorus were measured using enzyme-linked immunosorbent assay (ELISA), immunoassay and atomic absorptiometry respectively. BMD at the lumbar spine and proximal femur was determined by dual energy X-ray absorptiometry. RESULTS: The annual percent changes of BMD were not associated with single or combined genotypes of OPG and RANKL gene polymorphisms, and the distributions of these genotypes were not different between HT-responders and HT-nonresponders (women who lose more than 3% of bone mass per year). After HT of 6 months, change in serum sRANKL levels was significantly higher in GG genotype than in other genotype of RANKL gene polymorphism. No differences in the 6 month changes of other bone turnover markers including circulating OPG levels after HT were noted across single OPG genotype and combined genotypes of OPG and RANKL polymorphisms. CONCLUSIONS: The OPG T245G, and G1181C polymorphisms, and RANKL polymorphism did not associate with change in BMD after HT in postmenopausal Korean women, and RANKL polymorphism affects change in circulating sRANKL levels after HT.
Absorptiometry, Photon
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Alkaline Phosphatase
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Bone Density*
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Enzyme-Linked Immunosorbent Assay
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Female
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Femur
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Genotype
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Humans
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Immunoassay
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NF-kappa B*
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Osteocalcin
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Osteoprotegerin*
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Parathyroid Hormone
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Sequence Analysis, DNA
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Spine
9.The relationship between matrix Gla protein gene polymorphism, serum osteocalcin levels and bone mineral density in postmenopausal Korean women.
Jung Gu KIM ; Byung Chul JEE ; Seung Yup KU ; Chang Seok SUH ; Seok Hyun KIM ; Young Min CHOI ; Shin Yong MOON
Korean Journal of Obstetrics and Gynecology 2006;49(6):1304-1312
OBJECTIVE: To investigate the relationship between the matrix Gla protein (MGP) gene polymorphism, serum osteocalcin levels and bone mineral density (BMD) in postmenopausal Korean women. METHODS: The MGP gene cytosine-adenine (CA) polymorphism was analyzed by polyacrylamide-urea gel eletrophoresis, genescan and DNA sequencing in 267 postmenopausal Korean women. Serum osteocalcin, bone alkaline phosphatase (BAP), and CrossLaps (CTX) were measured by immunoassay, and enzyme linked immunosorbent assay respectively. BMD at the lumbar spine and proximal femur was determined by dual energy X-ray absorptiometry. RESULTS: Six MGP alleles were observed with product sizes ranging between 204-214 bp, and their distributions were as follows: 210 bp 57.5%, 221 bp 27.9%, 206 bp 13.3% etc. There were no significant differences in BMD of the lumbar spine and proximal femur across the MGP genotypes. No significant differences in the distribution of MGP genotypes among normal, osteopenic, and osteoporotic postmenopausal women were observed. Serum osteocalcin level was significantly higher in women who did not 210 bp MGP (CA) allele than in women carrying at least one copy of this allele, while no significant differences in serum BAP and CTX levels were noted among MGP genotypes. CONCLUSION: The MGP gene (CA) polymorphism does not affect BMD but serum osteocalcin levels in postmenopausal Korean women.
Absorptiometry, Photon
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Alkaline Phosphatase
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Alleles
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Bone Density*
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Enzyme-Linked Immunosorbent Assay
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Female
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Femur
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Genotype
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Humans
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Immunoassay
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Osteocalcin*
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Sequence Analysis, DNA
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Spine
10.Effect of alendronate on bone remodeling around implant in the rat.
Ran PARK ; Jee Hwan KIM ; Hyunmin CHOI ; Young Bum PARK ; Han Sung JUNG ; Hong Seok MOON
The Journal of Advanced Prosthodontics 2013;5(4):374-381
PURPOSE: The purpose of this study was to evaluate the effect of alendronates on bone remodeling around titanium implant in the maxilla of rats. MATERIALS AND METHODS: The maxillary first molars were extracted and customized-titanium implants were placed immediately in thirty male Sprague-Dawley rats. The rats were divided into experimental (bisphosphonate) group and control group. At 4 weeks after implantation, the rats in the bisphosphonate group were subcutaneously injected with alendronate three times a week for 6 weeks where as the rats in control group were injected with saline. The rats were sacrificed at 1, 2, 3, 4, or 6 weeks after starting of injection and maxillary bones were collected subsequently. Alveolar bone remodeling around the implants were evaluated by radiographic and histologic analysis. Microarray analysis and immunohistomorphologic analysis were also performed on one rat, sacrificed at 6 weeks after starting of injection, from each group. Statistical analysis was performed using repeated measures analysis of variance and independent t test at a significance level of 5%. RESULTS: There was no statistically significant difference in the bone area (%) around implant between the bisphosphonate group and the control group. However, the amount of empty lacuna was significantly increased in the bisphosphonate group, especially in the rats sacrificed at 4 weeks after starting of injection compared to that of the corresponding control group. The bisphosphonate group showed the same level of TRAP positive cell count, osteocalcin and angiopoietin 1 as the control group. CONCLUSION: Alendronate may not decrease the amount of osteoclast. However, the significantly increased amount of empty lacuna in the bisphosphonate group may explain the suppression of bone remodeling in the bisphosphonate group.
Alendronate*
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Angiopoietin-1
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Animals
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Bisphosphonate-Associated Osteonecrosis of the Jaw
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Bone Remodeling*
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Cell Count
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Humans
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Jaw
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Male
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Maxilla
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Microarray Analysis
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Molar
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Osteocalcin
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Osteoclasts
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Rats*
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Rats, Sprague-Dawley
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Titanium