2.Isolation of reovirus from SARS patients in China in 2003.
Chinese Journal of Epidemiology 2004;25(12):1078-1080
3.The isolation and identification of novel duck reovirus.
Shao-Ying CHEN ; Shi-Long CHEN ; Feng-Qiang LIN ; Shao WANG ; Bin JIANG ; Xiao-Xia CHENG ; Xiao-Li ZHU ; Zhao-Long LI
Chinese Journal of Virology 2012;28(3):224-230
The virus strains were isolated from the liver and spleen of the dead young ducks characterized with symptoms of hemorrhagic-necrotic hepatitis. These isolates could cause the death of muscovy duck-embryo and chick-embryo. 1-day-old birds infected with these isolates had the same character with clinically dead birds and the virus could be isolated from artificially infected birds. These isolates could proliferate in MDEF and result in CPE. The virus could proliferate in the cytoplasm in order of crystals and arranged in the latlic-like. The viron was shown spherical, icosahedron, cubic symmetry, no-envelope, with double-layered capsid, about 70 nm in diameter by electron microscopy. The genome segments of the virus were consisted of L1-3, M1-3 and S1-4, which were similar to that of avian reovirus (ARV). Compared to 68.2%, 69.3% - 70.1%, respectively. The system evolution analysis showed that S3 gene coding sigmaB protein was placed in different branch of MDRV and ARV, indicating that S3 gene of the virus was different from ARV and MDRV. The main clinical symptoms and lesions of ducklings caused by the virus were different from the diseases caused by MDRV and ARV. It was concluded that the virus was a Novel duck reovirus belonging to Orthoreovirus genus of the Reoviridae family.
Animals
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Animals, Wild
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virology
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Bird Diseases
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pathology
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virology
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Chick Embryo
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China
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Ducks
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Molecular Sequence Data
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Orthoreovirus, Avian
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classification
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genetics
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isolation & purification
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Phylogeny
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Reoviridae Infections
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pathology
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veterinary
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virology
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Viral Proteins
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genetics
4.Gene sequence analysis and prokaryotic expression of sigmaB protein of Muscovy duck reovirus YB strain.
Xiao-Ping WU ; Hong-Xing ZHANG ; Yi-Jian WU ; Dian-Lin HAN ; Shao WANG ; Bao-Cheng WU ; Yi-Fan HUANG
Chinese Journal of Virology 2013;29(2):185-191
Muscovy ducks reovirus (DRV) is an important pathogen with a high mortality rate in Muscovy ducks, the researches in the test and the immunity were useful for the prevention and control of DRV infection. In this study, the S3 genes of the three Fujian DRVs were cloned by RT-PCR and sequencing technology. It was found that DRV-YH and YJL were close to avian reovirus (ARV) in the genetic distance, with high identities ranged from 94. 6% to 98. 9%, however, the identities of DRV-YB strain and reference ARV strains in the S3 gene were only 60.6% - 61.7%. The expression vector pET-30a-S3 harboring DRV YB strain S3 gene was constructed and transformed into E. coli BL21, and then the fusion sigmaB protein expression was induced with IPTG. The SDS-PAGE of the expressed products indicated that the fusion protein of approximately 42ku in molecular weight was expressed highly in inclusion body, and made up 67. 7% of the total proteins. The most efficient concentration of IPTG and inducing time were 0. 1 mM and 5h respectively, while the best temperature for expression was 37 degrees C. After purification with the Ni2+ affinity chromatography, the fusion sigmaB protein was 93% of the total proteins, and the purified protein amounted to 0. 86g/L. The Western blot analysis showed that the fusion aB protein was recognized specifically by the antiserum against DRV, confirming that the recombinant fusion protein had good immunoreactivity.
Amino Acid Sequence
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Animals
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Capsid Proteins
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chemistry
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genetics
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metabolism
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Escherichia coli
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genetics
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metabolism
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Gene Expression
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Molecular Sequence Data
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Orthoreovirus, Avian
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chemistry
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classification
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genetics
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isolation & purification
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Phylogeny
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Poultry Diseases
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virology
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RNA-Binding Proteins
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chemistry
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genetics
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metabolism
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Recombinant Proteins
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genetics
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metabolism
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Reoviridae Infections
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veterinary
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virology
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Sequence Analysis
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Sequence Homology, Amino Acid