1.A novel M2e-multiple antigenic peptide providing heterologous protection in mice.
Feng WEN ; Ji Hong MA ; Hai YU ; Fu Ru YANG ; Meng HUANG ; Yan Jun ZHOU ; Ze Jun LI ; Xiu Hui WANG ; Guo Xin LI ; Yi Feng JIANG ; Wu TONG ; Guang Zhi TONG
Journal of Veterinary Science 2016;17(1):71-78
Swine influenza viruses (SwIVs) cause considerable morbidity and mortality in domestic pigs, resulting in a significant economic burden. Moreover, pigs have been considered to be a possible mixing vessel in which novel strains loom. Here, we developed and evaluated a novel M2e-multiple antigenic peptide (M2e-MAP) as a supplemental antigen for inactivated H3N2 vaccine to provide cross-protection against two main subtypes of SwIVs, H1N1 and H3N2. The novel tetra-branched MAP was constructed by fusing four copies of M2e to one copy of foreign T helper cell epitopes. A high-yield reassortant H3N2 virus was generated by plasmid based reverse genetics. The efficacy of the novel H3N2 inactivated vaccines with or without M2e-MAP supplementation was evaluated in a mouse model. M2e-MAP conjugated vaccine induced strong antibody responses in mice. Complete protection against the heterologous swine H1N1 virus was observed in mice vaccinated with M2e-MAP combined vaccine. Moreover, this novel peptide confers protection against lethal challenge of A/Puerto Rico/8/34 (H1N1). Taken together, our results suggest the combined immunization of reassortant inactivated H3N2 vaccine and the novel M2e-MAP provided cross-protection against swine and human viruses and may serve as a promising approach for influenza vaccine development.
Animals
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Antibodies, Viral/blood
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Antigens, Viral/genetics/*immunology
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Body Weight
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Cross Protection/*immunology
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Disease Models, Animal
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Epitopes, T-Lymphocyte/genetics/immunology
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Female
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Influenza A Virus, H3N2 Subtype/genetics/*immunology
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Influenza Vaccines/*immunology
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Mice
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Mice, Inbred BALB C
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Orthomyxoviridae Infections/*immunology/mortality/pathology/prevention & control
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Peptides/genetics/*immunology
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Random Allocation
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Survival Analysis
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Vaccines, Synthetic/immunology
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Virus Replication