Humans ; Organometallic Compounds ; chemistry ; metabolism ; toxicity

BACKGROUND: We evaluated the analytical performances of Diasys reagents manufactured by Diasys Diagnostic Systems (Holzheim, Germany) with Hitachi 747. METHODS: We evaluated AST, ALT, ALP, gamma-GT, calcium, and glucose. Only two tests were adapt-ed to different methods from the current ones: the photometric test using the arsenazo III for calci-um, the kinetic colorimetric test according to Szasz/Persijin using l-gamma-glutamyl-3-carboxy-4-nitranilide as a substrate for gamma-GT. Precision, interference, linearity, and method comparisons were evaluated by NCCLS guidelines. RESULTS: The coefficient of variation (CV) of total precision was less than 6.8% in all items. Preci-sions and linearities of all items were acceptable. Correlation coefficients were more than 0.9884 and all items showed excellent agreement compared to the current reagents in the reportable range. We could not find any significant interference for six test items up to 750 mg/dL triglyceride, 50 mg/dL hemoglobin, and 20 mg/dL bilirubin, except that the latter ALT showed a negative bias by more than 5 mg/dL of bilirubin. CONCLUSIONS: Diasys reagents showed high precision, linearity and correlation in comparison to the current reagents. So we conclude that these reagents are good for routine clinical use with Hitachi 747.
Arsenazo III ; Bias (Epidemiology) ; Bilirubin ; Calcium ; Glucose ; Indicators and Reagents* ; Triglycerides

An antral web is an extremely rare gastric anomaly that disturbs the gastric outlet. The onset of symptoms will depend on the diameter of the aperture. Obstructive symptoms may not occur when the aperture is >l centimeter in diameter. If the aperture is larger than 1 cm without significant symptoms, conservative treatment is sufficient. A case of an antral web with an ulcer and vomiting in a 7-year-old boy who received ibuprofen for 2 days is presented. The patient became symptom-free after medical treatment.
Carbamates ; Child ; Humans ; Ibuprofen ; Organometallic Compounds ; Stomach Ulcer ; Ulcer ; Vomiting

The maxillary sinus is an anthropological landmark because of difference of volume of maxillary sinus among persons of different ethnic origin. This study was undertaken to provide morphological characteristics of Korean's maxillary sinus. The size and volume of maxillary sinus were measured in 44 Korean skulls 23 males and 21 females from cadaver. The shapes of the maxillary sinus were classified according to Anagnostopoulou et al. (1991) and the sizes of the medial antral wall area of the maxillary sinus were classified according to Fernandes (2004b). The mean volume of maxillary sinus was 12.83+/-5.41 cm3, which was significantly different between males and females. Bilateral symmetry of the volume of the maxillary sinus was observed in the 70% of the specimens. The width of maxillary sinus was 26.59+/-4.96 mm. The height of maxillary sinus was 26.65+/-6.16 mm. The anteroposterior length was 32.84+/-4.96 mm. The anteroposterior length measured on C-Arm radiography was 36.99+/-4.72 mm. The shape of maxillary sinus was semi-ellipsoid and the majority (50%) of the shape of medial antral wall area of maxillary sinus was the type of small. The results of this study could be useful to clarify the anthropological characteristics of the maxillary sinus in Korean, and to provide an index of ethnic classification for the medial antral wall area.
Cadaver ; Carbamates ; Female ; Humans ; Male ; Maxillary Sinus ; Organometallic Compounds ; Skull

Adult ; Female ; Humans ; Male ; Occupational Diseases ; Organometallic Compounds ; poisoning

Cutaneous leishmaniasis is a skin infection caused by the Leishmania species, an intracellular protozoan parasite that is transmitted by various species of female sandflies. According to the geographic distribution and vectors, leishmaniasis is classified as Old World or New World cutaneous leishmaniasis. In Korea, 24 cases of Old World cutaneous leishmaniasis have been reported, but New World cutaneous leishmaniasis has not been reported as yet. A 37-year-old man presented with a 3-month history of a painful and erythematous nodule with two satellite papules on the left postauricular area and a papule on the left arm after traveling to the Amazon region in Brazil. After we performed skin biopsies of the lesions, diagnosis of cutaneous leishmaniasis was made by the histopathological findings. After intralesional injection of sodium stibogluconate (Pentostam(R), GlaxoSmithKline) twice a week for 4 weeks, the lesions improved with scarring. Herein, we discuss this case of New World cutaneous leishmaniasis that was successfully treated with intralesional injection of sodium stibogluconate (Pentostam(R)) in Korea.
Antimony ; Antimony Sodium Gluconate ; Arm ; Biopsy ; Brazil ; Cicatrix ; Female ; Humans ; Injections, Intralesional ; Korea ; Leishmania ; Leishmaniasis ; Leishmaniasis, Cutaneous ; Parasites ; Psychodidae ; Skin

Cutaneous leishmaniasis is a skin infection caused by the Leishmania species, an intracellular protozoan parasite that is transmitted by various species of female sandflies. According to the geographic distribution and vectors, leishmaniasis is classified as Old World or New World cutaneous leishmaniasis. In Korea, 24 cases of Old World cutaneous leishmaniasis have been reported, but New World cutaneous leishmaniasis has not been reported as yet. A 37-year-old man presented with a 3-month history of a painful and erythematous nodule with two satellite papules on the left postauricular area and a papule on the left arm after traveling to the Amazon region in Brazil. After we performed skin biopsies of the lesions, diagnosis of cutaneous leishmaniasis was made by the histopathological findings. After intralesional injection of sodium stibogluconate (Pentostam(R), GlaxoSmithKline) twice a week for 4 weeks, the lesions improved with scarring. Herein, we discuss this case of New World cutaneous leishmaniasis that was successfully treated with intralesional injection of sodium stibogluconate (Pentostam(R)) in Korea.
Antimony ; Antimony Sodium Gluconate ; Arm ; Biopsy ; Brazil ; Cicatrix ; Female ; Humans ; Injections, Intralesional ; Korea ; Leishmania ; Leishmaniasis ; Leishmaniasis, Cutaneous ; Parasites ; Psychodidae ; Skin

OBJECTIVE: To construct a HEK293 cell line stably overexpressing TrxR1 as a cell model for functional study of TrxR1 and screening of TrxR1-targeting drugs. METHODS: TrxR1 gene was amplified by PCR and ligated with the lentivirus expression vector pLVX-Puro, which was transformed into Escherichia coli and identified by Sanger dideoxy sequencing. HEK293 cells were infected with the recombinant lentivirus vector (pLVX-Puro-TXNRD1) and screened with Puromycin for cell clones with stable TrxR1 overexpression (HEK293-TrxR1-OE cells). HEK293-TrxR1-OE cells, along with HEK293 cells infected with pLVX-Puro vector (HEK293-NC) and normal HEK293 cells, were tested for mRNA and protein expression levels of TrxR1 using RT-qPCR and Western blotting. TrxR1 enzyme activity in the cells was evaluated with insulin endpoint assay and TRFS-green probe imaging. The sensitivity of the cells to auranofin, a specific TrxR1 inhibitor, was determined with CCK8 assay. RESULTS: TrxR1 gene was successfully inserted into the lentiviral vector pLVX-Puro as confirmed by DNA sequencing. The enzyme activity and mRNA and protein expression levels of TrxR1 were significantly higher in HEK293-TrxR1-OE cells than in HEK293 and HEK293-NC cells (P < 0.005). The inhibitory effects of auranofin on proliferation and cellular TrxR1 enzyme activity were significantly attenuated in HEK293-TrxR1-OE cells as compared with HEK293 and HEK293-NC cells (P < 0.005). CONCLUSION We successfully obtained a HEK293 cell line with stable TrxR1 overexpression, which shows resistance to auranofin and can be used for screening TrxR1 targeting drugs.
Auranofin ; Cell Line, Tumor ; Genetic Vectors ; HEK293 Cells ; Humans ; Lentivirus/genetics* ; RNA, Messenger ; Transfection

PURPOSE: This study was conducted to analyze the clinical course of patients of gastric cancer with outlet obstruction by comparing them with the gastric cancer patients without outlet obstruction. METHODS: The records of gastric cancer patients with outlet obstruction and who underwent radical gastrectomy from January 1996 to December 2005 were retrospectively reviewed and compared with those patients with gastric cancer and who were without outlet obstruction. RESULTS: The number of the patients who underwent radical gastrectomy was 461. Out of 461 patients, the number of gastric cancer patients with outlet obstruction was 42 (9.1%), and they had nasogastric tube insertion for an average of 4.4 days before operation. These patients showed several significant features such as large tumor size, advanced stage, an increased the number of involved LNs, a low serum albumin level, a frequent antral tumor location and duodenal invasion. There was no difference in complication rates between the two groups, but a higher mortality rate was noted in the gastric cancer patients with outlet obstruction than that in the other patients (P=0.038). The survival rate after the operation was significantly lower among the gastric cancer patients with outlet obstruction (P=0.0008), whereas no difference was found in the survival rate between the above two groups at the same stage (P=0.1951). CONCLUSION: The gastric cancer patients with outlet obstruction showed a comparatively poorer prognosis than those gastric cancer patients without outlet obstruction. However, there was no significant difference in the complication rate and the survival rate for both types of patients who were at the same stage. These results suggest that the patients with outlet obstruction can show the same result as the patients without outlet obstruction by undergoing radical gastrectomy.
Carbamates ; Gastrectomy ; Humans ; Organometallic Compounds ; Prognosis ; Retrospective Studies ; Serum Albumin ; Stomach Neoplasms ; Survival Rate

PURPOSE: The aim of this study was to examine the incidence, location and morphology of antral septa using radiographic exam.(Panorama, CT) in the dentate/non-atrophic and edentulous/atrophic maxillary segments. MATERIAL AND METHODS: 232 sinuses were subdivided into two groups(group1 : 175 sinuses were classified as complete & partial dentate maxillary segments, group2 : 57 sinuses were classified as complete edentulous maxillary segments) and were investigated for the incidence, location and morphology of maxillary sinus septa. RESULTS: A total of 80 septa were observed in 232 maxillary sinuses, which corresponded to 30.65% of the sinuses(71 of 232). 67.5% of the total septa was observed in the complete & partial dentate groups, but 32.5% of the total septa was observed in the complete edentulous group. Upon analysis of the anatomical location of the septa, it was seen that 14 septa(17.5%) were located in the P1 area, 15 septa(18.7%) were located in the P2 area, 19 septa(23.8%) were located in the M1 area and 32 septa(40%) were located in the M2 area and dista area of M2. we found 45 septa in the apical lesion of teeth, Group 1. However the remaining 9 septa were found in the edentulous area. CONCLUSION: CT is a better method than panoramic radiography for detecting the presence of the maxillary sinus septa. Maxillary sinus septa are more commonly detected in complete edentulous maxillary segments than in complete and partial dentate maxillary segments. Also maxillary sinus septa are more commonly detected posteriorly than anteriorly.
Carbamates ; Dental Implants ; Incidence ; Maxillary Sinus ; Organometallic Compounds ; Radiography, Panoramic ; Tooth