OBJECTIVETo compare and appraise the quality of germplasm resource of Ophiopogon japonicus in Sichuan basin.

METHODAccording to the main contents and yield traits, 24 wild germplasm resources of O. japonicus from different areas of Sichuan basin were comprehensively compared by the SPSS 17.0 software with principal component analysis and cluster analysis.

RESULTThe six samples of Ziyang, Jianyang, Leshan, Yibin, Chongqing, Mianyang, their comprehensive evaluation value of quality were higher than the others, and the sample of Ziyang had the best quality, the sample of Dazhou had the least quality, the results of the cluster analysis to raw data were also shown a similar results as principal component analysis.

CONCLUSIONThe wild resources of O. japonicus in Sichuan basin is rich, there are much differences among their quality; the method, through principal component analysis to study the comprehensive evaluation of the O. japonicus quality, is reliability and the results of cluster analysis is also support the conclusions, it could be able to provide a reference to select high O. japonicus quality resources.

Cluster Analysis ; Ophiopogon ; chemistry ; Principal Component Analysis

OBJECTIVETo study the effects of soil factors on the active components in Radix Ophiopogonis.

METHODThe content of polysaccharide, flavonoids, saponins, water-soluble extract and inorganic elements in Radix Ophiopogonis gathered from 7 different places were compared, physical and chemical properties and inorganic elements of soil were analyzed. The path and grey connection analysis were applied for studying the effects on the active components of Radix Ophiopogonis.

RESULT AND CONCLUSIONThe concentrations of mineral elements in plant were mainly adjusted by active absorption. The active components in Radix Ophiopogonis was primarily effected by soil enzyme activity, the other important factors were potassium, pH, and organic matter. K, Fe, Mn, B, Ba, Zn of soil also had much influence on it then others inorganic elements.

Flavonoids ; analysis ; Ophiopogon ; chemistry ; Plant Extracts ; analysis ; Polysaccharides ; analysis ; Soil ; analysis

Three homoisoflavonoids, 6-aldehydo-3-ophiopogonanone A, methyl ophiopogonanone A and ophiopogonanone A from Ophiopogon japonicus were analyzed simultaneously by HPLC with acetonitrile-water containing 0.5% H3 PO4 (58:42) as the mobile phase, and the detection wavelength was set at 296 nm (0-14 min) and 275 nm (14-22 min). The mean recoveries of three homoisoflavonoids were 99.41%-99.86% (RSD 0.82%-1.05%). The linear response ranges of. 6-aldehydo-3-ophiopogonanone A, methyl ophiopogonanone A and ophiopogonanone A were 0.165-0.990 microg (r = 0.999 9), 0.153-0.918 microg (r = 0.999 9), and 0.270-1.620 microg (r = 0.999 9), respectively. This method was certified to be accurate and reliable and can be used for quality control of O. japonicus.
Chromatography, High Pressure Liquid ; methods ; Drugs, Chinese Herbal ; analysis ; Isoflavones ; analysis ; Ophiopogon ; chemistry

OBJECTIVETo compare the agronomic characters, HPLC fingerprints, the content of main component and amino acid between erective and creepy Ophiopogon japonicus of Sichuan.

METHODAgronomic characters were measured by conventional methods; HPLC was applied on a C18 column with CH3OH-CH3CN-2% CH2COOH solution by gradient elution, quercetin was used as the internal standard reference, the contents of total saponins, flavone and polysaccharide were determined by UV spectrophotometry and amino acid was determined by automatic amino acid analyzer.

RESULTThere were extremely significant differences in the most agronomic characters between erective and creepy O. japonicus of Sichuan. The yield per plant was closely relevant to the roots number and the fresh weight of aerial part. The differences were not significant both in 69 common peaks and 23 uncommon peaks in HLPC fingerprints and the content of main component between erective and creepy O. japonicus.

CONCLUSIONThere is no obvious difference in chemistry component between the two types of O. japonicus. The yield per plant was closely relevant to the roots number and the fresh weight of aerial part. In cultivation it is appropriate to choose the creepy O. japonicus.

Amino Acids ; analysis ; Biomass ; China ; Flavones ; analysis ; Ophiopogon ; chemistry ; growth & development ; Plant Extracts ; analysis ; Polysaccharides ; analysis

Dengzhan Shengmai Capsules( DZSMC),a well-known traditional Chinese medicine( TCM) formula,is comprised of the main drug of Erigeron breviscapus,and supplemented with Panax ginseng,Ophiopogon japonicus and Schisandra chinensis,with functions of supplementing Qi and nourishing Yin,promoting blood circulation and strengthening brain. DZSMC is the only Chinese patent drug with A-level evidence-based medicine in secondary prevention for stroke and ranks first among TCMs for neurological treatment. Modern studies indicate that the chemical constituents of DZSMC mainly include flavonoids,phenolic acids,lignans,saponins and so on. Pharmacological experimental studies have shown that DZSMC has such pharmacological effects as anti-oxidation,anti-inflammatory and anti-myocardial ischemia. DZSMC is mainly used in the convalescent care of ischemic cardiovascular and cerebrovascular diseases,and is often used in combination with various conventional therapeutic drugs to exert clinical efficacy through brain protection,neuroprotection,etc.,and improve clinical symptoms in patients. In this review,according to domestic and international related literature combined with research results obtained by our project,the research advances in the chemical constituents,pharmacological effects and clinical application of DZSMC have been systematically reviewed and summarized,providing reference and support for further study and secondary development of the formula.
Drugs, Chinese Herbal/pharmacology* ; Erigeron/chemistry* ; Humans ; Medicine, Chinese Traditional ; Ophiopogon ; Panax ; Phytochemicals/pharmacology* ; Phytotherapy ; Schisandra

Nonaqueous capillary electrophoresis is used for the determination of the contents of diosgenin and ruscogenin in Radix Ophiopogonis. The operating buffer was composed of 20 mmol x L(-1) Na2B4O7-HCl (pH 7.61) in 70% methanol. The applied voltage was 25 kV and detection potential was at +0.70 V. With these conditions, the components were successfully separated. The content of diosgenin in Radix Ophiopogonis was 0.018 mg x g(-1) and ruscogenin was 0.008 mg x g(-1). The average recoveries of diosgenin and ruscogenin were 102% and 99.2%, respectively. A new method of the quality control of diosgenin and ruscogenin in Radix Ophiopogonis is provided.
Diosgenin ; analysis ; Electrophoresis, Capillary ; methods ; Ophiopogon ; chemistry ; Plant Roots ; chemistry ; Plants, Medicinal ; chemistry ; Quality Control ; Spirostans ; analysis

AIMTo separate and purify the anti-myocardial ischemic polysaccharide fraction with a homogenous molecular weight from Ophiopogon japonicus, then study the chemical structure of the parts.

METHODSCrude polysaccharides were prepared by extracting the tube root fraction of Ophiopogon japonicus with water, then precipitation with ethanol. From the crude polysaccharides, the polysaccharide of MDG-1 was separated and purified using ultrafiltration, DEAE Sepharose FF and Sephadex G-25 column chromatography. Its structure was studied by complete hydrolysis, periodate oxidation, Smith degradation, methylation analysis, 1H NMR and 13C NMR analysis etc.

RESULTS AND CONCLUSIONMDG-1 was a water-soluble beta-D-fructosan, containing a backbone composed of Fruf (2 --> 1), and a branch of Fruf (2 --> 6) Fruf (2 --> per average 2. 8 of main chain residues. Mn, Mw and Mp of MDG-1 were 3 400, 4 800 and 5 000, respectively. MDG-1 contains trace of Glc, which maybe connect to its reducing terminal. Molar ratio of Fru and Glc is approximately 35: 1.

Methylation ; Molecular Structure ; Molecular Weight ; Ophiopogon ; chemistry ; Plant Tubers ; chemistry ; Plants, Medicinal ; chemistry ; Polysaccharides ; chemistry ; isolation & purification

This research is to establish an HPLC method for the simultaneous determination of ophiopogonin D, ophiopogonin D', ophiopogonin C, deacetylophiopojaponin A and ophiogenin-3-O-α-L-rhamnosyl-(1-->2)-β-D-glucoside in Ophiopogonis Radix. HPLC-ELSD analysis was performed on a Kromasil 100-5 C₁₈ column (4.6 mm x 250 mm, 5 µm), with the mobile phase of acetonitrile (A) -water (B) in gradient elution mode (0-45 min, 35%-55% A), at a flow rate of 1 mL · min⁻¹. The column temperature was 35 °C and the drift tube temperature was 100 °C in a gas flow rate of 3.0 L · min⁻¹. The result showed that baseline of all the 5 constituents was well separated, and every constituent had wide linearity range and good linear relation (r > 0.999). The recovery rate was between 95.75% and 103.1%. The new established method for simultaneous determination of saponin constituents in Ophiopogonis Radix was sensitive and has good, repeatability. It could be applied to quality evaluation of Ophiopogonis Radix.
Chromatography, High Pressure Liquid ; methods ; Drugs, Chinese Herbal ; chemistry ; isolation & purification ; Ophiopogon ; chemistry ; Plant Roots ; chemistry ; Saponins ; chemistry ; isolation & purification

The chromatographic fingerprint was established for evaluating and controlling the quality of germplasm resource of Ophiopogon japonicus in Sichuan Basin by reversed-phase high performance liquid chromatography and gas chromatography mass spectrometry. The results showed that each sample the characterized by the peak area of 28 HPLC peaks and 13 GC peaks in each program and these peaks were employed for hierarchical cluster analysis. Furthermore, the discrimination of the sample from different regions was achieved by hierarchical cluster analysis via recognizing the 16 (41 datamatrix. This was the first report of hierarchical cluster analysis of the wild germplasm resource of Ophiopogon japonicus according to their chemical fingerprints. Thus, the results proved it is a simple, rapid and accurate method suitable for the quality control of the traditional Chinese medicines.
Chromatography, High Pressure Liquid ; methods ; Cluster Analysis ; Gas Chromatography-Mass Spectrometry ; methods ; Medicine, Chinese Traditional ; Ophiopogon ; chemistry

OBJECTIVETo study the patterns of dynamic accumulation of total flavonoids and total saponins in the roots of Ophiopogon japonicus collected from different harvest times, and compare the contents of total flavonoids and total saponins in roots of O. japonicus which were processed with different methods.

METHODThe total flavonoids and total saponins contents in O. japonicus were determined by ultraviolet spectrophotometry.

RESULT AND CONCLUSIONFrom December to January, the total contents of flavonoids and saponins in the roots of O. japonicus gradually decreased, and gradually increased from February to March, and kept stable in April. The contents of total flavonoids and total saponins in the O. japonicus were influenced by different processing methods.

Desiccation ; Flavonoids ; analysis ; metabolism ; Ophiopogon ; chemistry ; metabolism ; Plant Roots ; chemistry ; metabolism ; Saponins ; analysis ; metabolism ; Seasons ; Temperature ; Time Factors