No abstract available.
Breast Neoplasms* ; Breast* ; Diagnosis*

Objective To establish a high performance liquid chromato-graphic method for determining the concentration of baicalin in healthy volunteers.Methods The assay was conducted on the Agilent HC-C18 (250 mm × 4.6 mm,5 μm)column with methanol-acetonitrile-phosphoric acid (47 ∶ 53∶0.2)as the mobile phase.The detection wave length was set at 280 nm,and wogonin was taken as the internal standard.Results The linearity range was 0.25～ 12.0 μg/ml with a regressive equation of Y=0.0094 C-0.00175 (r=0.9997).The recovery was above 80％,with both intra-day RSD and inter-day RSD below 10％.Conclusion The method was convenient,rapid,accurate,and suitable for the pharmacokinetic study of baicalin and monitoring its concentration of clinical application.

Congenital heart disease (CHD) is the most common defect in human.The genetic basis of CHD is rather complicated.Chromosome aberrations,single gene disorders and polygenic disorders are three known genetic mechanisms that are responsible for cardiac malformations.Recently,with the application and development of molecular genetic approaches in CHD,significant advances in the understanding of the pathogenesis of cardiac malformations are being made.

Horseradish peroxidase was injected into C_(6,7) or L_(5,6) spinal cord in 21 adult cats and the morphology and distribution of the labeled cells in the dorsal columu nuclei (DCN) were studied. Cells projecting to the cervical cord were found to be distributed mainly in a region from 2.5mm below to 0.5mm above the obex, while cells projecting to the lumbar cord were found within 2.5mm below the obex. Medio-Iaterally, the cervical projecting cells were located chiefly at the junctional area between the gracile and medial cuneate nuclei and at the ventromedial part of the latter. The lumbar projection cells were located more medially, concentrating at the junctional area and the ventrolateral part of the gracile nucleus. In both cases scattered cells were found in the two dorsal column nuclei. The difference in cellular distribution between cervical and lumbar injection cases is consistent with a somatotopical organization.The labeled cells in the dorsal column nuclei varied in shape and size. Small cells, mostly fusiform, were concentrated at the junctional area between the gracile and medial cuneate nuclei. Large cells were found scattered in the two nuclei. Many of them were triangular or multipolar with long and straight dendrites. A few were round and their dendrites bushy.

HRP was injected into the cervical or lumbar spinal cord in 8 adult cats. The anterogradely labeled terminal arborization and its relation with the retrogradely labeled cells in the DCN were studied.The non-primary afferent fibers were distributed diffusely in the DCN. Injection of HRP in one spinal segment, either cervical or lumbar, resulted in labeling of terminal branches throughout the rostrocaudal extent of the DCN. In C6 or C7 injection cases large amount of labeled terminal branches were found in the medial cuneate nucleus, chiefly in its extraclusteral regions, viz. in the rostral part and in the ventral area of the caudal two thirds of the nucleus. A small celled area at the dorsolateral brim of the middle part of the medial cuneate nucleus was found to approach and to be contiguous with the external cuneate nucleus at higher level. This area was likewise densely labeled. Labeled terminal branches were also found in the gracile nucleus in lesser amount.After L5 or L6 injections the labeled terminals were much less in amount than in cervical injection cases. They were distributed mainly in the gracile nucleus. In the lower part of the nucleus the labeled terminals were found in its dorsal, medial, and ventrar areas. In the upper part of the nucleus they projected diffusely. A slight degree of labeling was also found in the ventromedial angle of the medial cuneate nucleus.The areas of distribution of the labeled terminals and labeled cells overlapped but did not coincide with eachother. No or merely a few labeled terminals were found around some of the labeled cells, while other cells were located right in the center of heaviest terminal labeling with abundant terminal branches surrounding their somata and dendrites. This proved that the non-primary afferent fibers of the DCN might form monosynaptic feed-back loop with the DGN-spinal cells.

HRP was injected into the cervical or lumbar spinal cord in 8 cats and the spinobrainstem projections were traced with the anterograde HRP technique. The labeled terminal branches were found most densely concentrated in the ipsilateral dorsal column nuclei and lateral reticular nucleus, the contralateral medial and dorsal accessory olivary nuclei and the bilateral pontobulbar bodies and dorsolateral part of pontine nuclei. In the reticular formation and a number of brainstem nuclei labeled terminals were also found to varying degrees. The most remote site found labeled was the hypothalamus.It was also found that the dorsal accessory olivary nucleus could be further subdivided into a caudal and an oral part, cells in the caudal part being smaller than that in the oral one.In the accessory olivary nuclei labeled terminal branches were more numerous in lumbar injection cases. Clearcut somatotopical localization was demonstrated in the oral part of the dorsal accessory olivary nucleus.In the lateral reticular nucleus the subnucleus magonocellularis and the lateral wing of the subnucleus parvocellularis were the major sites of labeling and the labeling in cervical injection cases greatly out numbered that in lumbar injections.The cervical and lumbar injections were equal in their amount of projection to the pontobulbar bodies and the dorso-lateral part of the pontine nuclei.The distribution of spinal afferents in the inferior olivary nucleus, the lateral reticular nucleus, the pontobulbar body and the pontine nucleus was discussed.

Objective To identify the changes in proliferative activity and cytotoxicity of lymphokine activated killer(LAK) cells after in vitro co-culture with dendritic cells from umbilical cord blood(CBDC). Methods CBDCs were induced by using GM-CSF and IL-4,and then co-cultrued with allogenic LAK at different proportions.Proliferative activity and cytotoxicity of LAK cells were measured by MTT assay.Results The typical CBDCs were induced in vitro,and the cultured CBDCs stimulated the proliferation and cytotoxicity of LAK cells.The optimal proportion of CBDC and LAK was 1:10,at which the activated LAK showed the highest cytotoxic effect on the tumor cells.Conclusion The CBDCs can enhance the proliferation and cytotoxicity of LAK cells.

Different parts of the cochlea were destroyed in 6 cats (11 sides). The degenerating fibers and preterminal degenerations were studied by the Nauta technique with the following conclusions: 1. The overwhelming majority of the fibers from the spiral ganglion project to within the limit of the cochlear nucleus complex. No fiber degeneration could be found in the trapezoid body. A few degenerating fibers from the basal turn of the spiral ganglion, however, were found in the dorsal acoustic stria. 2. No preteriminal degeneration was found in the “granular cell masses” and “dorsal cell complex of the acoustic tubercle”of G. Fuse. 3. In the dorsal cochlear nucleus, degenerating fibers were found to terminate in the II-V layers. 4. Contrary to Lorente de No’s finding, the present study showed that the fibers from the basal portion of the spiral ganglion bifurcate at the dorsal part of the ventral cochlear nucleus, those from the apical portion at the ventral part. 5. The spiral ganglion projects in a clear-cut topographical way to the 3 parts of the cochlear nucleus complex, viz, the basal portion and the successively more apical portions of the spiral ganglion project (a) to the dorso-medial and successively more ventro-lateral parts of the dorsal cochlear nucleus; (b) to the anterior part of the ventral cochlear nucleus along the dorso-caudo-medial to ventro-rostro-lateral axis; and (c) to the posterior part of the ventral cochlear nucleus along the dorso-rostro-medial to ventro-caudo-lateral axis.

OBJECTIVE:To observe the clinical efficacy of the combination of traditional Chinese medicine with western medicine in the treatment of recurrent genital herpes and to observe the recurrence rate of this disease.METHODS:A total of 120 patients with recurrent genital herpes were randomly assigned to 3 groups:the combination group received Chinese herbal medicine plus western medicine Valaciclovir capsule(0.25 g,bid,po for 30 d)and Recombinant human interferon;the western medicine group received Valaciclovir capsule and Recombinant human interferon,while the Chinese herbal medicine group received self-designed Chinese herbal medicine.RESULTS:The clinical efficacy was higher and the recurrence rate was lower in the combination group than in the other two groups(all P

Hemostatic experiments were taken by melting the Fugu gelatin with calcium chloride powder on the femoral artery bleeding of the laboratory animals. The powder prescription Gel-B showed better and more effects on stopping the femoral arterial hemorrhage than that of A-25, A-7 hemostats and gauge hemostat, with repetiton rate 87.03% at 6 minutes. There were no allergeic and poisoning reactions on experiments animals.