Introduction: Although the physic-chemical nature and compounds of Otgontenger spring have been relatively studied over the last years, no any substantial scientific work was done in regard to its therapeutic action and significance.It is known that during gastritis, chronic hepatitis and rheumatism macro and micro elements and biologically active compounds dramatically decrease within cells of each related organ and the processes of cell’s bioenergy, bio-synthesis, and re-synthesis [1.2] are imbalanced that leads to loss of cell’s divisibility and its rejuvenescence. Consequently, disbalance in blood coagulation, micro-circulation, ischemia, hypothermia, reperfusion substantial decrease of endurance to anti-oxidant deficiency and anti-radical activity weakness are observed. So, our study has been done basing on our hypothesis that macro and micro elements as well as biologically active compounds in Otgontenger resorts as sulphate ion (SO42-), azotes (N2), hydrosulfuric acid (H2S), oxygen (О2), carbonate ion (СO32-), chloral ion (CL-), hydrogen (Н2), carbonic acid (СО2,) could replace the biologically regulative agents which are cardinally shortened and becoming the main factor of pathologies [3. 4].Goal: The goal of this study is to investigate treatment activity “Otgontenger” resorts treatment experimental conditions. It is purposed to define the redoxy-potentiality and anti-radical activities of Otgontenger resorts.Materials and Methods: The study was conducted at the Central Scientific Laboratory of Monos Medical University between 2009-2011. Otgontenger mineral water was transported in a package accordingly to the Mongolian Standard “The Technical requirements to mineral water of treatment and drinking intent” (MNS 3651: 2005). In order to determine the anti-radical activity of the mineral water each portion of the studying water is dissolved in ethanol. After that 0.1 and 0.2 mlg are taken from these solutions and are added to 1.5 mlg of alfa, alfa-demetilpicril-gydralice suspension. The solution is shaken well and once color deeply changed it was defined at 517 HM through spectrophotometer by B.Yu. Kulikov’s method (1995, 1996). In regard to Osmos’s endurance property, 16 white experimental rabbits are taken by mouth 1.0 mlg/200 grams each from the mineral water number 1 of the 1st group, number 18th of the 4th group and number 47th of the 7th group respectively two times a day for the consecutive five days. The blood is taken and centrifuged in a way that serum is segregated and 0.5 mlg of 10 percent erythrocytes is separated. Consequently, 5.0 mlg of distilled water is added and hymolize is carried out. Once gymolize is fully done, supernatant is separated after centrifuge and absorption is measured at 420hm by spectrophotometer. In regard to defining hydropereoxide endurance, the white rabbit is taken by mouth 1.0 mlg/200 grams each from the mineral water number 1 of the 1st group, number 18th of the 4th group and number 47th of the 7th group respectively two times a day for the consecutive five days. The blood is centrifuged and serum is segregated. The blood is taken and centrifuged in a way that serum is segregated and 0.5 mlg of 10 percent erythrocyte is separated. Consequently, 3.0 mlg of Н202 is added and hymolize is carried out. Once gymolize is fully done, supernatant is separated after centrifuge and absorption is measured at 420hm by spectrophotometer.Results: It has been defined that antiradical activity in the erythrocytes’ 4th compartment of the white rabbits taken mineral water number 18th of the 4th group and number 1st of the 1st group increased by 1.11-1.12 times compared to the group not taken, whereas, decreased by 1.1 times by the impacts of the mineral water under 47th number of the 7th group (Table 1). It is shown that the mineral water has effects to increase proton flows at mitochondria membranes and donators’ oxidation, because antiradical activity of the second compartment of the rabbit’s erythrocytes has gone down by 1.13-1.16 times compared to the group not taken because of the mineral water under number 18 of the 4th group and number 1 of the 1st group and number 47 of the 7th group. If we see table 2 and picture 2, it is proved that mineral water has effects to accelerate oxygen flow to erythrocytes or acceptors’ diffusion and as well energy supply because membrane’s peroxide endurance of erythrocytes’ fourth compartment of the white rabbit decreased by 1.11-1.12 times under the influence of Otgontenger resorts number 18 and 1 of the 4th and 1st groups respectively and as well osmos’s endurance property of membrane of the erythrocytes’ 4th compartment decreased by 1.2-1.22 times compared to those not taken it. If we see picture 3, it is proved that mineral water has effects to accelerate oxygen flow to erythrocytes or acceptors’ diffusion and energy supply because membrane’s peroxide endurance of erythrocytes fourth compartment of the white rabbit decreased by 1.11-1.12 times under the influence of Otgontenger resorts with numbers 18 and 1 of the 4th and 1st groups respectively and as well osmos’s endurance property of membrane of the erythrocytes’ 4th compartment decreased by 1.2-1.22 times compared to those not taken it.From picture 4 it can be seen that under the influence of some types of Otgontenger mineral water as 18th number of the 4th group and the first number of the 1st group MDA contained in the second compartment of the white rabbit decreases by 1.11-1.12 times compared to the control group not taken and as well osmoses’ endurance of the erythrocytes’ 4th compartment membrane declines by 1.2-1.22 times which means they have effects to accelerate oxygen flow to erythrocytes or acceptors’ diffusion and consequently energy supply. We can see from picture 5 that under influence of mineral water with number 47 of the 7th group, the oxidise-ferment activity of erythrocytes’ second compartment serum has decreased two times for the control group compared to the ones not taken the mineral water. Moreover, the oxidise-ferment activity of erythrocytes’ first compartment declined by 1.2 times as a result of the mineral water with number 47 of the 7th group. That means that the mineral water suppresses the ferment activity of lipoproteidlipaza but makes the flow of redoxy-line protons accelerated.Conclusion: It has been defined that Otgontenger mineral water has high antitoxic effects to reduce free radicals like DPHH.

Introduction. Garidi-5, a traditional medicine composed of 5 herbs including Terminalia chebula Retz., Aconitum Kusnezoffii Reichb., Acorus calamus L., Saussurea lappa L., and musk of Moschus moschiferus, has been used in traditional Mongolian medicine as an analgesic and antibacterial medicine. The present work was undertaken to evaluate the traditional drug Garidi-5 for its analgesic and anti-inflammatory activity.Materials and Methods. The method of Winter et al. was used to study acute inflammation. Rats in groups of five each were treated with vehicle, Garidi-5 (20, 80 and 200 mg/kg, p.o.) and Indometacin (10 mg/kg) one hour prior to Carrageenan injection. 0.1 ml of 1% Carrageenan was injected into the subplantar tissue of left hind paw of each rat. Swelling of carrageenan injected foot was measured at 0, 0.5, 2, 4 h using Plethysmometer (UGO Basile, Italy)). The right hind paw was injected with 0.1 ml of vehicle.Results. The Garidi-5 (20, 80 and 200 mg/kg) significantly (P<0.01) inhibited carrageenan induced rat paw edema as compared to control group. Maximum inhibition of paw edema was observed with Garidi- 5 (80 and 200 mg/kg) at 4 h when compared to the control group (Tab.1). In assay data, the TNF-α, IL-1β and IL-6 secretion in serum were highly elevated by carrageenan induction but administration of Garidi-5 signifi cantly reduced serum secretion of inflammatory mediatorsas compared to vehicle group (Tab. 2).Conclusion. In conclusion, Traditional drug Garidi-5 have anti-in flammatory properties. The potential efficacy of Garidi-5 to treat inflammation is based in a part on the hy pothesis that it will suppress the proinflammatory cytok ines resulting in less oedema.

Introduction. Garidi-5, a traditional medicine composed of 5 herbs including Terminalia chebula Retz. Aconitum Kusnezoffii Reichb., Acorus calamus L., Saussurea lappa L., and musk of Moschus moschiferus, has been used in traditional Mongolian medicine as an analgesic and antibacterial medicine. The present work was undertaken to evaluate the traditional drug Garidi-5 for its analgesic and anti-inflammatory activity.Materials and Methods. The method of Winter et al. was used to study acute inflammation. Rats in groups of five each were treated with vehicle, Garidi-5 (20, 80 and 200 mg/kg, p.o.) and Indometacin (10 mg/kg) one hour prior to Carrageenan injection. 0.1 ml of 1% Carrageenan was injected into the subplantar tissue of left hind paw of each rat. Swelling of carrageenan injected foot was measured at 0, 0.5, 2, 4 h using Plethysmometer (UGO Basile, Italy)). The right hind pawwas injected with 0.1 ml of vehicle.ResultsThe Garidi-5 (20, 80 and 200 mg/kg) significantly (P<0.01) inhibited carrageenan induced rat paw edema as compared to control group. Maximum inhibition of paw edema was observed with Garidi-5 (80 and 200 mg/kg) at 4 h when compared to the control group (Tab.1). In assay data, the TNF-α, IL-1β and IL-6 secretion in serum were highly elevated by carrageenan induction but administration of Garidi-5 signifi cantly reduced serum secretion of inflammatory mediators as compared to vehicle group (Tab. 2). ConclusionIn conclusion, Traditional drug Garidi-5 have anti-in flammatory properties. The potential efficacy of Garidi-5 to treat inflammation is based in a part on the hy pothesis that it will suppress the proinflammatory cytok ines resulting in less oedema.

Background: In recent years, scientists have found that certain natural compounds have significant potential in cancer prevention and early-stage cancer treatment. Flavanones, a class of polyphenolic compounds found in plants, vegetables, seeds, fruit peels, and flowers, have been identified to possess anticancer, antioxidant, anti- inflammatory, and antibac terial bioactivities. Cancer has become a major global challenge in terms of both economic and public health concerns. Global statistics indicate that 22.8% of deaths are attributed to non-communicable diseases, and 16.8% are caused by cancer, accounting for one in four and one in six deaths, respectively. Aim : To investigate anticancer effects of Iris Tenuifolia-derived flavanone on cancer cell lines. Materials and Methods : The study was conducted at the Bio-Medical Research Institute of the Mongolian National Uni versity of Medical Sciences, investigating the effect of flavanones on cancer cell viability under in vitro conditions using the MTT assay. In the study, colon, liver, and lung cancer cells were cultured, stabilized, and used for the experiments. Colorectal cancer cells (MC38), liver cancer cells (HepG2), and lung cancer cells (A549) were revived, cultured, and stabilized for use in the experimental procedures. Statistical analysis of the results was performed using Microsoft Excel 2010, and graphs were generated using GraphPad Prism 8. Differences between groups were analyzed using Student’s t-test, and a p-value of <0.05 was considered statistically significant. Results : We treated MC38, HepG2, and A549 cancer cells with different concentrations of flavanone (2.5 µM, 5 µM, and 10 µM) for 24 to 48 hours to evaluate cell viability. Flavanone inhibited A549 cell viability by 2.5 μM-10%, 5 μM-25%, and 10 μM-38%, respectively. For HepG2 cells, flavanone treatment at concentrations of 5-10 µM reduced cell viability by 28–58%. No statistically significant effect on the viability of MC38 cells was observed following treatment with fla vanone at concentrations ranging from 2.5 to 10 µM. Additionally, although MC38 inhibited cell viability in a dose-de pendent manner in cell cultures, it had a statistically significant effect at higher concentrations of 30-200 μM (p<0.01). Conclusion Flavanone inhibits the cancer cell viability in a dose and time dependent manner