Recent development of oral and maxillofacial surgery has taken an interest in the regeneration of facial bone defects. For the reconstruction of bony defects, autogenousbone grafts have been widely used. However, there are some clinical problems ; the morbidity of donor sites, resorption of autogenous bone graft and the availability of the proper form and size. The purpose of this study is to find the proper filling materials for the bone defects. The present study was designed to evaluate the roles and regenerating capacity of maikture of freeze-dried domineralized allogenic bone and hydroxylapatite. Fifteen adult New Zealand white rabbits were used as the experimental animal, Four trephine defects were made by drilling on the parietal bone of each rabbit. The size of each defects was 8x8mm. In first group, the defect was filled with freeze-dried demineralized allogenic bone and hydroxylapatite. The defect in second group was filled with autogenous bone chip only. The third group was filled with autogenous bone chip and hydroxylapatite. The fourth group was filled with freeze-dried dimineralized allogenic bone only. The results were carefully examined grossly and micorsocopically from the 2 weeks to 12 weeks postoperatively. The following results were obtained ; 1. In the case of the graft with autogenous bone chip only, new bone formation was more active than any other groups. 2. In the case of the graft with combination of freeze-dried demineralized allogenic bone and hydroxylapatite, new bone formation was similar to the case of autogenous bone and hydroxylapatite mixture. 3. In the case of the graft with freeze-dried demineralized bone, new bone formation was observed, but less than the other groups. And the time of new bone appeared was later than the other groups. A combination of freeze-dried deminearalized allogenic bone and hydroxylapatite plays an effective role in forming new bone. The results of this study may suggest the possible usage of the mixture of freeze-dried allogenic bone and hydroxylapatite as an alternative to autogenous bone in maxillofacial orthopedic surgery.
Adult ; Animals ; Bone Regeneration* ; Durapatite* ; Facial Bones ; Humans ; Orthopedics ; Osteogenesis ; Parietal Bone ; Rabbits ; Regeneration ; Surgery, Oral ; Tissue Donors ; Transplants*

Colchicine has been shown to regulate the expression of inflammatory gene, but this compound possesses much weaker anti-inflammatory activity. In this study, we synthesized a new colchicine derivative CT20126 and examined its immunomodulatory property. CT20126 was found to have immunosuppressive effects by inhibiting lymphocyte proliferation without cytotoxicity and effectively inhibit the transcriptional expression of the inflammatory genes, iNOS, TNF-alpha, and IL-1beta, in macrophages stimulated by LPS. This effect was nearly comparable to that of cyclosporine A. This compound also significantly suppressed the production of nitric oxide and Th1-related pro-inflammatory cytokines, IL-1beta, TNF-alpha, and IL-2, with minimal suppression of Th2-related anti-inflammatory cytokines IL-4 and IL-10 in the sponge matrix allograft model. Moreover, administration of CT20126 prolonged the survival of allograft skins from BALB/c mice (H-2d) to the dorsum of C57BL/6 (H-2b) mice. The in vivo immune suppressive effects of CT20126 were similar to that of cyclosporine A. These results indicate that this compound may have potential therapeutic value for transplantation rejection and other inflammatory diseases.
Animals ; Cell Line ; Colchicine/*analogs & derivatives/chemistry/*pharmacology ; Cytokines/*biosynthesis ; Female ; Gene Expression Regulation/drug effects ; Graft Survival/*drug effects ; Immunosuppression ; Interleukin-1beta/genetics/metabolism ; Lipopolysaccharides/pharmacology ; Lymphocyte Culture Test, Mixed ; Mice ; Mice, Inbred BALB C ; Mice, Inbred C57BL ; Nitric Oxide/biosynthesis ; Nitric Oxide Synthase Type II/genetics/metabolism ; Skin Transplantation/*immunology ; Th1 Cells/*drug effects/immunology/metabolism ; Th2 Cells/*drug effects/immunology/metabolism ; Transplantation, Homologous ; Tumor Necrosis Factor-alpha/genetics/metabolism