Abstract In the past 30-40 years, the use of herbal medicines, preparations and herbal finished products have extensively increased worldwide. The lifestyle and life quality of the world population have changed with the following longevity. People become a concern to use effective, qualitative, safety, with fewer side effects naturally derived medicine and products for healthcare, to protect and cure various diseases. Therefore, it is very essential to develop standards for medicinal plant materials and crude drugs, including herbal medicine and finished products. Many factors influence the quality, safety, efficiency, availability and popularity of herbal medicine. Among them, the quality of medicinal plant materials and supplementary compounds are very important.
In this paper, the reasons and requirements for the standardization of medicinal plant materials and crude drugs, permissible and limited quantities of the quality and quantity of biologically active compounds, as well as and contaminants and residues are described.

Introduction: Plant species of Pedicularis L., one of the largest genera of the Scrophulariaceae family, is found in cold regions of Asia, Europe, and America. In ancient Chinese, Tibetan, and Indian medicinal literature, it is noted that due to the detoxification potential of Pedicularis L., it has been used for reducing and curing of edema, fever, inflammation and poisoning. In the recent studies, species of Pedicularis L. have been determined to possess anti-oxidative, antitumor, antibacterial, antifatigue and, analgesic activities, as well as muscle relaxant, hepatoprotective effects. From this genus, more than 230 constituents, mostly belonging to the phenylpropanoids, iridoids, lignans and their glycosides have been isolated and identified. Material and method: In this review, we reviewed 80 papers on the genus Pedicularis L. which were published in scientific journals from 1963 to 2018, using databases such as Springer Link, Google Scholar, Pub Med, Elsevier, Research Gate. In addition, literatures on Mongolian medicinal plants and herbal medicines have been used, where the taxonomy, morphology of Pedicularis L. and their usage was described. Conclusions As this survey, less than 10 percent of the 600 species of Pedicularis L. has been studied for their phytochemical constituents and biological activities. More than 230 compounds including phenylpropanoids, iridoids, lignans, flavonoids, alkaloids and other classes of constituents have been isolated and their molecular structures were identified. Among them, 67 compounds or 30 percent were natural new compounds. Anti-oxidative, antibacterial, antitumor and, muscle relaxant activities of the Pedicularis L. related to the quantities and structural peculiarities of phenylpropanoid glycosides, iridoid glycosides, and flavonoids. Especially, phenylpropanoid glycosides are more interesting as they demonstrate wide range and much higher biological activity compared to other classes of compounds. Therefore, future research will focus on chemistry and biological activity of this class of compounds.

Abstract More than 100 species of the Lactuca genus (Compositae family) are distributed across the northern hemisphere and also in temperate and warm regions of the world, namely 17 species in Europe, 51 in Asia, 43 in Africa, 12 in North and South America. In the Mongolian flora, four species of Lactuca, Lactuca unduiata Ledeb. Lactuca tatarica C.A.M, Lactuca serriola L. and Lactuca sibirica L Benth ex Maxim., are found. Their chemical constituents and biological activities have not been thoroughly studied. Plants of Lactuca species are widely used in traditional medicine, to treat acute respiratory infection and they have antipyretic and analgesic activities.
Since the 1980's, chemical constituents of Lactuca species started to be studied. Currently, about 148 secondary metabolites from more than 20 species were isolated and their structures elucidated, including sesquiterpene lactones-65 (43.91%), flavonoids-20 (13.51%), phenolic compounds-26 (17.56%), coumarins-2 (1.35%), triterpenes and steroids-20 (13.51%), lignans-5(3.39%), and other class of compounds-10 (6.75%). Among the isolated secondary metabolites, 9 were registered as new natural compounds. Extracts and isolated pure compounds from Lactuca species showed hepaprotective, antidiabetic, anticancer and antioxidant activities.

Introduction: Due to social transition and change of many societal activities in Mongolia, there is an increasing need for pharmaceutical drugs to treat anxiety, stress, fatigue and insomnia. It is considered that medicinal plants used in traditional medicine as sedatives, anti-anxiety and anti-depressive activities could be effective. Our study focuses on exploring optimal methods for preparing tinctures from Paeonia L. genus species, a sedative in Mongolian traditional medicine, and studying their phytochemical compositions. Materials and methods: Root and herb of Paeonia anomala L., grown in Mogod soum in Bulgan province, were used for the study.
Prepared herb and root were chopped and sieved into 1 mm, 2 mm, 3 mm, 4 mm and 5 mm sizes. Then from each size of two samples, a set was created with a 1:1 ratio. Finally 40 % ethanol (1:10 ratio with a set) was applied to prepare tincture through a traditional maceration method. The obtained tincture was analyzed for its color, quantity of biologically active compounds, dry residue, and absorption coefficient using an optimal particle size of samples, volume of the extractive liquid and tincture yield.
Tincture from the set of herb and root of Paeonia anomala L. was prepared using 3 methods, namely, by the traditional maceration; intensified maceration using the ultrasonic bath processing with 45 kHz at an ambient temperature; constant shaking in a heat of 30o-40oC. Conclusion The more optimal and effective method for preparing tincture from Paeonia anomala L. has been found to be the ultrasonic bath processing with 45 kHz for 6 h at an ambient temperature. Crude drug mixture of 2 mm sizes with 40% ethanol (where volume absorption coefficient was accounted to be 2.8) was mixed at a ratio of 1:10. In the obtained tincture, total phenols were determined as 0.58±0.003% in gallic acid equivalent and total monoterpene glycosides as 1.96±0.002% in paeoniflorin, which were the highest compared to other methods, indicating the method is the more optimal one.

Introduction: It is important to standardize a tincture prepared from the herb and root of Paeonia anomala L, which is widely used as a sedative in traditional medicine, based on the results of the studies its chemical composition, biological and pharmacological activities. Moreover, there is a need to carry out a quantitative stability testing in order to establish the ability to maintain quality under certain storage condition, shelf-life and to develop suitable packaging information. Materials and methods: Standardization parameters of prepared Paeonia anomala tincture have been evaluated according to WHO guidelines for the determination of crude drug material, plant preparations and finished products technical parameters, along with the Mongolian National Pharma- copoeian (MNP) and Russian XIV Pharmacopoeian methods for tincture. The color of a tincture, dry residue, special density, alcohol content, and microbiological content were determined according to the methods described in MNP. A qualitative analysis of biological active constituents has been evaluated by thin-layer chromatography, the total phenolic compounds were determined by the reaction with Folin Chicalteu reagent and total monoterpene glycosides by the reaction with hydroxylamine in alkaline solution and ferric (III) chloride following spectrophotometric examination, respectively.
The stability testing study was performed according to the “General requirements for the stability testing study of drug-active compounds” MNS-6439-2014 using the real-time methods for the standardization parameters of the tincture. Conclusions The standardized parameters of tincture prepared from the herb and root of Paeonia anomala L. were approved by the National Reference Laboratory for Food Safety analysis. Consequently, the Mongolian pharmacopoeian article on Paeonia anomala tincture was officially permitted under the number ҮФӨ-0327-2017.
The stability study of tincture was carried out according to the MNS-6439-2014 by real-time tests for 24 months which provided that the changes in tincture quality were within the permitted limits. Consequently, it can conclude that the storage condition and shelf-time of Paeonia anomala tincture are 2 years under not above 25°С.

Introduction: The development of analytical methods for evaluation of quality standard parameters of drugs and other health prophylactic, diagnostic, and therapeutic products is essential from the beginning in the process to release to the market finished product. Validation of developed analytical methods is one crucial part of the analysis.
Quantitative determination methods of the total main active compounds of the tincture prepared from the root and the herb of Paeonia anomala L. were developed. In order to verify whether the analytical procedure is suitable for its intended purpose parameters – accuracy, reproducibility, intermediate precision, and linearity were validated. Materials and methods: The total phenolics in the tincture were determined by the reaction with Folin Chicolteau reagent and total monoterpene glycosides by the reaction with hydroxylamine in alkaline solution and ferric (III) chloride following spectrophotometric examination, respectively. Based on the achieved results of the developed quantitative methods which were validated according to the accepted and pursued technical documents in our country the Eurachem guide “The Fitness for Purpose of Analytical Methods” and Russian Pharmacopoeia 13 “Validation of analytical methodology”, where parameters as accuracy, precision – reproducibility, intermediate precision, and linearity are involved. Conclusions Statistical analysis of total phenolic compounds and total monoterpene glycosides in the Paeonia anomala tincture demonstrated that the bias calculated was for accuracy 0.674% and 0.703%, relative standard deviations were for reproducibility 0.896% and 0.798%, for inter- mediate precision 0.922% and 0.696%, respectively and the linearity – R²=0.9985 and R²=0.9997. All achieved results validated the appropriateness of the developed quantitative methods for the intended purpose.

The dichloromethane fraction from the ethanol extract of the aerial parts of Taraxacum offi cinale showed a good inhibitory effect on hepatocellular carcinoma cells (HCC). As a result of a series of column chromatographies and usage of nuclear magnetic resonances spectrometric methods and mass spectroscopy 9 known components as taraxasterol (1), taraxasterylacetate (2),pseudotaraxasterol (3), lupeolacetate (4), b-sitosterol (5), b-sitosterylglucopyranose (6), palmitic acid (7), monopalmitin (8) and chrysoerol (9) have been determined. Amongst them palmitic acid, monopalmitin and chrysoeriol have been determined for the fi rst time in the aerial parts of Taraxacum offi cinale. Six compounds as 1, 3, 4, 5, 6 and 7 were tested for their inhibitory activity on HCC and only palmitic acid exhibited more activity against HCC than others, suppressing cell proliferation, migration, adhesion and activated cell apoptosis.Keywords: Triterpenol and sterol derivatives; palmitic acid; hepatocellular carcinoma inhibition activity;IntroductionTaraxacum, commonly called dandelion, is a large genus of fl owering plants in the Asteraceae family. The latin name Taraxacum is from the Greek and means “disease remedy”, while the English name dandelion is originated from the French dent de leon, meaning “lion’s tooth”1. The Mongolian well-recognised name is “baaban beeben”, while in Japan it calls hokouei, respectively. Consequently, Taraxacum is widespread plant throughout the world, in particular, 19 species are found in the Mongolian fl ora2. Generally Taraxacum is considered weedy plant used as a medicinal herb and for food preparation. Traditionally, Taraxacum offi cinale Weber ex Wigg. in Mongolian and Tibetan medicine under the name “khurmong” the root has been used as the composition in a remedy for jaundice and other disorders of the liver and gallbladder, whilethe leaf is used as a diuretic and bitter digestive stimulant. Moreover, fresh dandelion stem latex is used for the warts treatment1,3-6. Taraxacum leaf is included as a medicinal drug in Herbal Pharmacopeia of several European countries. Numerous biological activity tests resulted that Taraxacum possessed an infl ammation modulating activity7-9, diuretic activity comparable to furosemide10, digestive stimulant, appetitive effect and activator for bile fl ow11-12, hypoglycemic activity13 and antitumor activity14. No side effects and carcinogenicity of T. offi cinale extracts and preparations have been noticed. Chemical constituents of T. offi cinale arewell studied. Scientists of different countries reported that whole plant T. offi cinale containedabundance of bitter principles as terpenoids and sterols, bile like terpenes and sterols, various fl avonoids and phenolic acids, large amount of polysaccharides as inulin and fructosans11,15-17.Also, dandelion is a rich in minerals such as iron, potassium and zinc18,19. In this work we are describing activity-guided isolation and the molecular structure elucidation of components from the dichloromethane fraction of the aerial parts of T. offi cinale, from the Mongolian fl ora.

Introduction: Sophora alopecuroides L has broadly been utilized in traditional medicine and all crude drugs including root, herb, and seed are used to treat numerous diseases. This herb is included in 181 Tibetan-Mongolian medicinal prescriptions and ranks 8^th among Mongolian medicinal plants in terms of frequency of administration. The S.alopecuroidesL . root standard was developed by the Institute of Traditional Medicine and Technology in 2017 and approved by “ҮФӨ-0307-2017”. Herb and seed are still used in medicine. Therefore, their standard parameters need to be determined and verified. Materials and methods: The quantitative pharmacognosy analysis of herb and seed was carried out in accordance with the methodology specified in the “General requirements for medicinal plant raw materials” of the National Pharmacopoeia of Mongolia. To determine the total alkaloid in standard matrine, a bromcresol green complex was formed, which was measured by spectrophotometer. Conclusion By developing, standards for the crude drugs of herb and seed of S.alopecuroides L. which are included in numerous medicinal prescriptions, will confirm the rationale for the use of medicinal raw materials and to expand the utilization’s possibilities.

BACKGROUND OF STUDY: Not only in developed countries but also in Mongolia it is vital problem to decide how to improve human life’s quality, to make clear the measurement to take, discover human body’s change, aging mechanism and predict from these diseases. The research on herders’ biological aging which is connected to the geography, nature, environment, climate, inhabitant, culture and labor’s specialty hasn’t been done yet. So it causes the basis to write this research. AIMS: Defining the nomadic herder’s biological aging MATERIAL AND METHODOLOGY: All the herders in Gobi-Altai were included in this research. They wereclassified into ages such as 30-39, 40-49, 50-59 and 60-69, and 202 female and 212 male herders were chosen accidentally. The model types of research “Cross-Sectional” method is used for this, the equipment called “Tanita” which is used for measuring body is used for collecting information and also SPSS-22programmis used for producing result. RESULT OF STUDY: 1. 49% of all research participants are male, 51% are female. 2. Herder’s biological aging is 50,4+9,4 and it shows that 6,3 older than calendar ages. 3. Herders’ aging was different for male and female herders. 81,8 percent of the male herders get older very fast whereas 69,4 of female herders get older slowly. CONCLUSION: • Male herder’s aging is 14 years older than womenThe older female and male herders get, the less their difference in their biological age becomes. • The percentage of the herders whose aging is getting fast is 21-26 % older than biological aging of the UB city’s people. • Biological age and sex’s connection (r=0,0001 p=0,00488) between biological age and weight’s index is (r=0,00488, p=0,001) is different and statistical significant.