1.Comparison of Detection Methods and Culture Media for Isolation of Helicobacter pylori from Gastric Biopsy Specimens.
Sin Kyung KIM ; Eun Suk KIM ; Ile Kyu PARK ; Jung Oak KANG ; Tae Yeal CHOI
Korean Journal of Clinical Pathology 1997;17(6):1060-1067
BACKGROUND: Helicobacter pylori(H. pylori) has been implicated in the pathogenesis of chronic active gastritis and peptic ulcer disease. All patients with ulcers who are infected with H. pylori receive antimicrobial therapy. Therefore diagnosis of H. pylori infection is imperative for the treatment gastritis or ulcer patients. We evaluated the four diagnostic methods and three culture media for the isolation of H. pylori. MATERIALS AND METHODS: Rapid urease test(CLO test), modified Gram stain, culture, and nested polymerase chain reaction (PCR) were performed with 108 gastroscopic biopsy specimens from patients with peptic ulcer or chronic gastritis. Among them 40 specimens were inoculated onto each of 5% sheep blood agar, e99 Yolk emulsion (EYE) agar, and 7% horse blood agar containing antibiotics. RESULTS: The positive rates were the highest by the PCR(72%), 67% by modified Gram stain, 64% by CLO test, and 57% by culture. Among the three media the horse blood agar (selective medial) gave the highest isolation rate (48%), followed by sheep blood agar (45%), and EYE agar (38%). CONCLUSION: Though PCR was the most sensitive method for the detection H. pylori modified Gram stain was sensitive enough, simple, rapid, and economical as the routine diagnostic method of H. pylori. For the culture of H. pylori combination of sheep blood agar as nonselective media and horse blood agar as selective media would show the highest isolation rate.
Agar
;
Anti-Bacterial Agents
;
Biopsy*
;
Culture Media*
;
Diagnosis
;
Gastritis
;
Helicobacter pylori*
;
Helicobacter*
;
Horses
;
Humans
;
Peptic Ulcer
;
Polymerase Chain Reaction
;
Sheep
;
Ulcer
;
Urease
2.Trends in Rotavirus Gastroenteritis in Korea from 1989 through 1998 and comparison of Slidex Rota-kit 2 and VIDAS Rotavirus.
Jung Oak KANG ; Sun E KIM ; Think You KIM ; Iie Kyu PARK ; Tae Yeal CHOI
Korean Journal of Clinical Microbiology 1999;2(2):152-157
BACKGROUND: Rotavirus activity in Korea has been reported beginning in October, peak in November, continuing in winter and ending in spring. But the peak month and the incidence of rotavirus seems to be changed recently. So we investigated the trends of rotavirus activity for the last 10 years in Hanyang University Hospital (HUH). Also latex agglutination test was compared with automated enzyme-linked fluorescent immunoassay for the detection of rotavirus in stool specimens. METHODS: Stool specimens (3,636 from HUH, 1989-1998; 1,171 from Hanyang University Kuri hospital, HUKH, 1996-1998) from pediatric patients with acute diarrhea were tested for rotavirus,. Sixty specimens were tested by latex agglutination test (Sliders Rota-kit 2, bioMerieux Vitek, France) and enzyme-linked fluorescent immunoassay (VIDAS Rotavirus, bioMerieux Vitek, France) according to the instructions from the manufacturer. RESULTS: The annual incidence of rotavirus diarrhea from 1989 to 1998 was 47%, 32%, 33%, 25%, 26%, 24%, 24%, 17%, 17%, 14%, respectively. Positive rate of rotavirus was 25% for the 10year period in HUH, 20% for the recent 3 years in HUKH. Peak month was November (46%) in the first 5 year, but November incidence decreased to 17% in the last 5 year, and the peak moved to January, February, and March (34%, 35%, 33%, respectively). Epidemic period was from October to February during the first 5 year, but from December to April during the last 5 year period. The agreement rate of the two methods was 90% and VIDAS Rotavirus showed significantly higher sensitivity compared to Sliders Rota-kit 2. CONCLUSIONS: The incidence of rotavirus diarrhea decreased gradually for the last 10 years and the peak month of rotavirus activity was changed from November to January, February, and March. The VIDAS Rotavirus was more sensitive than the Sliders Rota-kit 2 for the detection of rotavirus in stool.
Diarrhea
;
Epidemiology
;
Gastroenteritis*
;
Humans
;
Immunoassay
;
Incidence
;
Korea*
;
Latex Fixation Tests
;
Rotavirus*
3.Mediating and Moderating Effects of Multicultural Efficacy in the Relationship between Cultural Empathy and Cultural Competence in Child Care Teachers
Won Oak OH ; Il Tae PARK ; Minju SONG
Child Health Nursing Research 2019;25(2):214-222
PURPOSE: This study examined the mediating and moderating effects of multicultural efficacy in the relationship between cultural empathy and cultural competence in child care teachers. METHODS: A cross-sectional descriptive survey design was used. The participants were 277 child care teachers at private and public daycare centers in G and S districts of Seoul. The survey instruments included a cultural empathy questionnaire, a multicultural efficacy scale, and a cultural competence scale. Data were analyzed using the SPSS and AMOS programs. For data analysis, descriptive statistics, correlation, and mediating and moderating effect analyses were performed. Bootstrapping was implemented to verify the mediating effect of the model developed herein. RESULTS: Positive correlations among cultural empathy, multicultural efficacy, and cultural competence were noted. Multicultural efficacy showed a significant mediating effect on the relationships between cultural empathy and cultural competence. However, there was no moderating effect. CONCLUSION: In order to enhance the cultural competence of child care teachers, it is necessary to develop a strategy that can promote their cultural empathy and multicultural efficacy. Furthermore, these results will ultimately enhance the role of child care teachers, thus contributing to the normal growth and development of multicultural children.
Child
;
Child Care
;
Child
;
Cultural Competency
;
Cultural Diversity
;
Empathy
;
Growth and Development
;
Humans
;
Negotiating
;
Self Efficacy
;
Seoul
;
Statistics as Topic
4.Evaluation of a child abuse prevention program for unmarried mothers in South Korea: a single-case experimental design
Child Health Nursing Research 2024;30(3):187-198
Purpose:
The purpose of this study is to apply and evaluate the effectiveness of a child abuse prevention program based on the Nursing Model of Resilience and Coping Skills Training Model for unmarried mothers during pregnancy and puerperium.
Methods:
This study had a prospective single-case, AB design with four repeated self-questionnaire measures and three observational measures. Seven unmarried mothers were provided with 10 sessions child abuse prevention program through individual visits from 32 to 34 weeks of pregnancy to 6 weeks after childbirth. The questionnaire was composed related to resilience, maternal stress, maternal attitude, parent-child interaction, child abuse potential. The observation was measured by video recording (total 16 times) the interaction of parent-child during feeding and analyzing it by three experts. Data were analyzed by Wilcoxon signed-rank test and Friedman’s test.
Results:
Maternal attitude and parent-child interaction were statistically significantly improved after intervention compared to before intervention. However, maternal stress decreased after intervention compared to before intervention, but it was not statistically significant. Also, resilience and child abuse potential were not statistically significant. This program is partially effective in preventing child abuse by promoting parenting attitudes and parent-child interactions.
Conclusion
This study focused on individual resilience and applied systematic intervention as coping skills training to prevent child abuse. This study is meaningful in that interventions were conducted through individual visits to unmarried mothers at high risk of child abuse, and the program was applied, including pregnancy and postpartum periods, to prevent child abuse early.
5.Peak Expiratory Flow(PEF) Measured by Peak Flow Meter and Correlation Between PEF and Other Ventilatory Parameters in Healthy Children.
Chul Ho OAK ; Kai Hag SOHN ; Ki Ryong PARK ; Hyun Myung CHO ; Tae Won JANG ; Maan Hong JUNG
Tuberculosis and Respiratory Diseases 2001;51(3):248-259
BACKGROUND: In diagnosis or monitor of the airway obstruction in bronchial asthma, the measurement of FEV1 in the standard method because of its reproducibility and accuracy. But the measurement of peak expiratory flow(PEF) by peak flow meter is much simpler and easier than that of FEV1 especially in children. Yet there have been still no data of the predicted normal values of PEF measured by peak flow meter in Korean children. This study was conducted to provide equations to predict the normal value of PEF and correlation between PEF and FEV1 in healthy children. METHOD: PEF was measured by MiniWright peak flow meter, and the forced expiratory volume and the maximum expiratory flow volume curves were measured by Microspiro HI 501(Chest Co.) in 346 healthy children (age:5-16 years, 194 boys and 152 girls) without any respiratory symptoms during 2 weeks before the study. The regression equations for various ventilatory parameters according to age and/or height, and the regression equations of FEV1 by PEF were derived. RESULTS: 1. The regression equation for PEF(L/min) was:12.6×age(year)+3.4×height(cm)-263(R2=0.85) in boys, and 6×age(year)+3.9×height(cm)-293(R2=0.82) in girls. 2. The value of FEFmax(L/sec) derived from the maximum expiratory flow volume curves was multiplied by 60 to compare with PEF(L/min), and PEF was faster by 125 L/min in boys and 118 L/min in girls, respectively. 3. The regression equation for FEV1(ml) by PEF(L/min) was:7×PEF-550(R2=0.82) in boys, and 5.8×PEF-146(R2=0.81) in girls, respectively. CONCLUSION: This study provides regression equations predicting the normal values of PEF by age and/or height in children. And the equations for FEV1, a gold standard of ventilatory function, was predicted by PEF. So, in taking care of children with airway obstruction, PEF measured by the peak flow meter can provide useful information.
Airway Obstruction
;
Asthma
;
Child*
;
Diagnosis
;
Female
;
Forced Expiratory Volume
;
Humans
;
Reference Values
6.Anatomical Correlates of Neuropsychological Deficits Among Patients With the Cerebellar Stroke.
Min A SHIN ; Oak Tae PARK ; Joon Ho SHIN
Annals of Rehabilitation Medicine 2017;41(6):924-934
OBJECTIVE: To investigate the anatomical correlates of the neuropsychological deficits in patients with the cerebellar stroke. METHODS: We screened patients who were admitted to the National Rehabilitation Center with the cerebellar stroke between October 2012 and November 2016. The patients with the cerebellar stroke who underwent neuropsychological testing for which the Seoul Neuropsychological Screening Battery (SNSB) or the SNSB-II were enrolled. The neuropsychological function capacities were compared in accordance with the stroke type (hemorrhagic vs. ischemic) and the location (right/left anterior, right/left posterior intermediate, right/left posterior lateral lobe, and vermis). Mean z-scores were computed to compare the patient performances with the population averages. RESULTS: Twenty-six patients (15 with ischemic stroke and 11 with hemorrhagic stroke) with a mean age of 54.8±16.6 years were assessed 8.8±9.2 months after the stroke. Differences in the neuropsychological functioning according to the stroke type were not observed. All of the numerical subtests of the stroke patients showed significantly poorer performances compared with the population averages (mean z-score < 0), and some of the subtests revealed abnormal performances in attention-, visuospatial function-, memory-, and frontal/executive function-related tasks (mean z-score <−1). The patients with the presence of a lesion in the right posterior intermediate lobe of the cerebellum showed a poorer performance in the subtests evaluating the executive function including the Korean-version Stroop Test (p=0.04), the Digit Symbol Coding Test (p=0.01), and the Korean-version Trail Making Test (p=0.02) compared with the patients without that lesion. CONCLUSION: The present study confirms that the cerebellar stroke affects the neuropsychological functioning which is associated with the anatomical site of stroke.
Cerebellum
;
Clinical Coding
;
Cognition
;
Executive Function
;
Humans
;
Mass Screening
;
Neuropsychological Tests
;
Rehabilitation Centers
;
Seoul
;
Stroke*
;
Stroop Test
;
Trail Making Test
7.Anatomical Correlates of Neuropsychological Deficits Among Patients With the Cerebellar Stroke.
Min A SHIN ; Oak Tae PARK ; Joon Ho SHIN
Annals of Rehabilitation Medicine 2017;41(6):924-934
OBJECTIVE: To investigate the anatomical correlates of the neuropsychological deficits in patients with the cerebellar stroke. METHODS: We screened patients who were admitted to the National Rehabilitation Center with the cerebellar stroke between October 2012 and November 2016. The patients with the cerebellar stroke who underwent neuropsychological testing for which the Seoul Neuropsychological Screening Battery (SNSB) or the SNSB-II were enrolled. The neuropsychological function capacities were compared in accordance with the stroke type (hemorrhagic vs. ischemic) and the location (right/left anterior, right/left posterior intermediate, right/left posterior lateral lobe, and vermis). Mean z-scores were computed to compare the patient performances with the population averages. RESULTS: Twenty-six patients (15 with ischemic stroke and 11 with hemorrhagic stroke) with a mean age of 54.8±16.6 years were assessed 8.8±9.2 months after the stroke. Differences in the neuropsychological functioning according to the stroke type were not observed. All of the numerical subtests of the stroke patients showed significantly poorer performances compared with the population averages (mean z-score < 0), and some of the subtests revealed abnormal performances in attention-, visuospatial function-, memory-, and frontal/executive function-related tasks (mean z-score <−1). The patients with the presence of a lesion in the right posterior intermediate lobe of the cerebellum showed a poorer performance in the subtests evaluating the executive function including the Korean-version Stroop Test (p=0.04), the Digit Symbol Coding Test (p=0.01), and the Korean-version Trail Making Test (p=0.02) compared with the patients without that lesion. CONCLUSION: The present study confirms that the cerebellar stroke affects the neuropsychological functioning which is associated with the anatomical site of stroke.
Cerebellum
;
Clinical Coding
;
Cognition
;
Executive Function
;
Humans
;
Mass Screening
;
Neuropsychological Tests
;
Rehabilitation Centers
;
Seoul
;
Stroke*
;
Stroop Test
;
Trail Making Test
8.Detection of Helicobacter pylori by Polymerase Chain Reaction.
Tae Yeal CHOI ; Jung Oak KANG ; Kyung Nam PARK ; Yiel Hea SEO
Korean Journal of Infectious Diseases 1997;29(5):371-376
BACKGROUND: Helicobacter pylori has been implicated in the pathogenesis of active chronic gastritis and peptic ulcer disease in man. Thus, diagnosis and treatment of H. pylori infection are now of growing importance in ulcer management. A variety of non-invasive and invasive methods have been described for the detection of H. pvlori, but all of these techniques have disadvantages such as time consuming or insensitivity. So we describe the polymerase chain reaction(PCR) assay for the sensitive and specific detection of H. pylori. METHODS: Gastric biopsy specimens were obtained from 247 patients undergoing endoscopic examinations at Hanyang University Hospital. One half of the specimen was processed for routine culture and the other half for PCR. Bacterial genomic DNA from gastric biopsies was extracted by Instagene. Two sets of primer pairs derived from the nucleotide sequence of the urease A gene of H. pylori were used. RESULT: H. pylori was cultured in 100(40%) cases and PCR assay detected 179 (72%) cases (P<0.05, Chi-square test). Culture and PCR-positive cases totaled 100, and there were 68 cases negative by both methods. There were 79 culture-negative and PCR-positive cases, but none was culture-positive and PCR-negative. The assay was sensitive for detecting as little as 0.1 pg of DNA (1 bacterial cell). The specificity of detection was confirmed by ensuring that the primers did not amplify DNA extract from other bacteria. CONCLUSION: The PCR is a rapid, accurate, and sensitive method for the detection of H. pylori.
Bacteria
;
Base Sequence
;
Biopsy
;
Diagnosis
;
DNA
;
Gastritis
;
Genes, vif
;
Helicobacter pylori*
;
Helicobacter*
;
Humans
;
Peptic Ulcer
;
Polymerase Chain Reaction*
;
Sensitivity and Specificity
;
Ulcer
;
Urease
9.Application of Cognitive Perceptual Assessment for Driving (CPAD) for the Brain Injured Patients: A preliminary study.
Soo Won CHOI ; Soon Ja JANG ; Si Woon PARK ; Jong Tae LEE ; Oak Tae PARK
Journal of the Korean Academy of Rehabilitation Medicine 2008;32(3):273-279
OBJECTIVE: To evaluate the utility of Cognitive Perceptual Assessment for Driving (CPAD) through the on-road test in predicting the actual road driving skills in brain injured patients. METHOD: 25 brain injured patients with actual driving experience before the occurrence of diseases participated in the handicapped driving adaptation training program of our hospital as the subjects of the study, and one round of CPAD and on-road test were performed. CPAD evaluations and on-road tests for all patients were evaluated by the same therapist. RESULTS: 11 patients passed the CPAD and all of them passed the on-road test. 3 patients failed from CPAD and they turned out to be failed at the on-road test. The mean CPAD score of the on-road test passed group was 53.6 which was significantly different from the mean 43.6 CPAD score acquired by the on-road test failed group by showing a significant difference between two groups (p<0.05). Among the 11 patients who received the borderline CPAD score, 9 patients passed the on-road test. CONCLUSION: CPAD is considered to be useful in predicting the actual road driving skills of brain injured patients who previously had driving experiences before the occurrence of the diseases.
Brain
;
Disabled Persons
;
Humans
10.A Case of Yersinia enterocolitica Enterocolitis and Mesenteric Lymphadenitis Diagnosed by Colonoscopy.
Kyung Nam PARK ; Dong Soo HAN ; Joo Hyun SOHN ; Seong Hee LEE ; Joon Yong PARK ; Jung Oak KANG ; Tae Yeal CHOI
Korean Journal of Gastrointestinal Endoscopy 1996;16(5):773-779
Yersinia enterocolitica is an important pathogen that causes a spectrum of clinical illnesses from simple gastroenteritis to invasive ileitis and colitis. Also it is important to differentiate endoscopic findings involving terminal ileum between inflammatory bowel diseases and infectious colitis. There were few reports about Y. enterocolitica infection confirmed by culture and serology in Korea. We report the first case, in Korea, of terminal ileitis and mesenteric lymphadenitis by Y. enterocolitica confirmed by colonoscopy, biopsy, tissue cultures, and serology in a 42-year-old woman complained vague abdominal discomfort and loose stool.
Adult
;
Biopsy
;
Colitis
;
Colonoscopy*
;
Crohn Disease
;
Enterocolitis*
;
Female
;
Gastroenteritis
;
Humans
;
Ileitis
;
Ileum
;
Inflammatory Bowel Diseases
;
Korea
;
Mesenteric Lymphadenitis*
;
Yersinia enterocolitica*
;
Yersinia*