Objective:To develop the microsurgical nursing quality standard, so as to provide reference for improving the nursing quality level of microsurgery.Methods:From March to August 2023, based on the theoretical framework of the three-dimensional "structure-process-outcome" quality model, the content and weight of microsurgical nursing quality standard were determined through literature review, semi-structured interviews, Delphi method and analytic hierarchy process.Results:The effective recovery rates of 2 rounds of expert consultations were 100.00%(24/24) and 95.83%(23/24). The authority coefficients were 0.931 and 0.928. The variation coefficients of each item ranged from 0.000 to 0.159 and from 0.000 to 0.135. The Kendall harmony coefficients were 0.076 and 0.091. Finally, the quality standard of microsurgery nursing included 3 first-level standards (structural quality criteria weight 0.200, process quality criteria weight 0.400, outcome quality criteria weight 0.400), 27 second-level standards and 87 third-level standards.Conclusions:Nursing quality standard of microsurgery is scientific, reasonable, specialized and operable, which can guide the clinical practice of specialized nurses and promote the improvement of microsurgical nursing quality.