Background: Chronic suppurative otitis media (CSOM) has caused many hearing disorder cases in developing countries.Inappropriate antibiotic use resulted in a shift of bacterial resistance. The biofilm-forming bacteria, like Pseudomonas aeruginosa, was a common germ detected in CSOM that contributed to a poor prognosis. This study aimed to investigate the bacterial pattern from samples taken from CSOM patients regarding its antibiotic susceptibility and the antibiofilm activity of acetic acid against P. aeruginosa. Materials and Methods: Sterile swabs of forty-five patients with CSOM were collected, followed by isolation of bacterial pathogens, identification, and evaluation of antibiotic sensitivity using modified Kirby Bauer disc diffusion protocol. In vitro testing was done by adding acetic acid to P. aeruginosa culture to gauge the minimum concentration of biofilm inhibition and eradication. They were conducted using the microtiter plate assay method and quantified with an ELISA reader. The data were analyzed statistically using One-Way ANOVA and Tukey Honestly Significant Difference post hoc test. Results: The samples obtained from 31 of 45 CSOM patients showed positive microbial growth; 26 (57.78%) had a monomicrobial pattern, and 5 (11.11%) had a polymicrobial pattern. The researcher ascertained that 24 isolates, representing 66.67%, were gram-negative bacteria, with P.-aeruginosa identified as the predominant species.P. aeruginosa isolates were sensitive to several antibiotics, including meropenem, amikacin, piperacillin-tazobactam, ceftazidime, and cefoperazone-sulbactam with a rate of 93.33%. The minimum concentration of acetic acid required to qualify as the minimum biofilm inhibitory concentration (MBIC) was determined to be 0.16%, yielding an inhibition rate of 26.79%. A concentration of 0.31% was identified as the minimum biofilm eradication concentration (MBEC), achieving an eradication rate of 77.27%. Conclusion P. aeruginosa, the most common bacteria found in CSOM samples, was sensitive to imipenem, amikacin, piperacillin-tazobactam, ceftazidime, and cefoperazone-sulbactam. Acetic acid suppresses P. aeruginosa bacterial biofilm formation at MBIC of 0.16% and MBEC of 0.31%.

Background: Chronic suppurative otitis media (CSOM) has caused many hearing disorder cases in developing countries.Inappropriate antibiotic use resulted in a shift of bacterial resistance. The biofilm-forming bacteria, like Pseudomonas aeruginosa, was a common germ detected in CSOM that contributed to a poor prognosis. This study aimed to investigate the bacterial pattern from samples taken from CSOM patients regarding its antibiotic susceptibility and the antibiofilm activity of acetic acid against P. aeruginosa. Materials and Methods: Sterile swabs of forty-five patients with CSOM were collected, followed by isolation of bacterial pathogens, identification, and evaluation of antibiotic sensitivity using modified Kirby Bauer disc diffusion protocol. In vitro testing was done by adding acetic acid to P. aeruginosa culture to gauge the minimum concentration of biofilm inhibition and eradication. They were conducted using the microtiter plate assay method and quantified with an ELISA reader. The data were analyzed statistically using One-Way ANOVA and Tukey Honestly Significant Difference post hoc test. Results: The samples obtained from 31 of 45 CSOM patients showed positive microbial growth; 26 (57.78%) had a monomicrobial pattern, and 5 (11.11%) had a polymicrobial pattern. The researcher ascertained that 24 isolates, representing 66.67%, were gram-negative bacteria, with P.-aeruginosa identified as the predominant species.P. aeruginosa isolates were sensitive to several antibiotics, including meropenem, amikacin, piperacillin-tazobactam, ceftazidime, and cefoperazone-sulbactam with a rate of 93.33%. The minimum concentration of acetic acid required to qualify as the minimum biofilm inhibitory concentration (MBIC) was determined to be 0.16%, yielding an inhibition rate of 26.79%. A concentration of 0.31% was identified as the minimum biofilm eradication concentration (MBEC), achieving an eradication rate of 77.27%. Conclusion P. aeruginosa, the most common bacteria found in CSOM samples, was sensitive to imipenem, amikacin, piperacillin-tazobactam, ceftazidime, and cefoperazone-sulbactam. Acetic acid suppresses P. aeruginosa bacterial biofilm formation at MBIC of 0.16% and MBEC of 0.31%.

Background: Chronic suppurative otitis media (CSOM) has caused many hearing disorder cases in developing countries.Inappropriate antibiotic use resulted in a shift of bacterial resistance. The biofilm-forming bacteria, like Pseudomonas aeruginosa, was a common germ detected in CSOM that contributed to a poor prognosis. This study aimed to investigate the bacterial pattern from samples taken from CSOM patients regarding its antibiotic susceptibility and the antibiofilm activity of acetic acid against P. aeruginosa. Materials and Methods: Sterile swabs of forty-five patients with CSOM were collected, followed by isolation of bacterial pathogens, identification, and evaluation of antibiotic sensitivity using modified Kirby Bauer disc diffusion protocol. In vitro testing was done by adding acetic acid to P. aeruginosa culture to gauge the minimum concentration of biofilm inhibition and eradication. They were conducted using the microtiter plate assay method and quantified with an ELISA reader. The data were analyzed statistically using One-Way ANOVA and Tukey Honestly Significant Difference post hoc test. Results: The samples obtained from 31 of 45 CSOM patients showed positive microbial growth; 26 (57.78%) had a monomicrobial pattern, and 5 (11.11%) had a polymicrobial pattern. The researcher ascertained that 24 isolates, representing 66.67%, were gram-negative bacteria, with P.-aeruginosa identified as the predominant species.P. aeruginosa isolates were sensitive to several antibiotics, including meropenem, amikacin, piperacillin-tazobactam, ceftazidime, and cefoperazone-sulbactam with a rate of 93.33%. The minimum concentration of acetic acid required to qualify as the minimum biofilm inhibitory concentration (MBIC) was determined to be 0.16%, yielding an inhibition rate of 26.79%. A concentration of 0.31% was identified as the minimum biofilm eradication concentration (MBEC), achieving an eradication rate of 77.27%. Conclusion P. aeruginosa, the most common bacteria found in CSOM samples, was sensitive to imipenem, amikacin, piperacillin-tazobactam, ceftazidime, and cefoperazone-sulbactam. Acetic acid suppresses P. aeruginosa bacterial biofilm formation at MBIC of 0.16% and MBEC of 0.31%.

Background and Objectives: The airport ground handling workers are most vulnerable to noise exposure from the sound of aircraft engines that are quite loud and, if exposed continuously, they may experience hearing loss. This study describes the profile of noise induced hearing loss in ground handling workers at Juanda Airport Surabaya.Subjects and Method The descriptive cross sectional approach is employed, and the noise level in the work environment is measured by airport apron, physical ear examination, distortion product otoacoustic emission (DPOAE) and pure tone audiometry, which were conducted for 89 ground handling workers at the airport. Results: The age range that experienced hearing loss the most is 50-59 years (19.10%). The male: female ratio of subjects who experienced hearing loss was 50%:53.24%. The highest percentage of subjects with hearing loss was found in the Area II (export-import cargo) with 68%. Workers who experience hearing loss the most have been working for 17-21 years (76%). The ratio of hearing loss between continuous users of ear protectors and occasional or non-users of protectors was 22.47%:30.34%. Of the 89 total research subjects, 47 were found with hearing based on the DPOAE refer results; of these subjects, 30 (33.7%) experienced hearing loss due to noise (NIHL), 9 suffered in the right ear (10.11%), 9 in the left ear (10.11%) and 12 in both ears (13.48%). Conclusion Based on the DPOAE results, 52.81% of the ground handling workers at Juanda airport Surabaya were found with hearing loss; of these workers, 33.7% workers experienced NIHL with 10.11% observed in each ear and 13.48% in both ears.

Introduction: Vertigo is estimated to occur in 3% of adults every year. In contrast to dizziness, vertigo is associated with symptoms of peripheral or central balance disorders, while dizziness is associated with cardiovascular, neuropathic, neuromuscular, or psychosomatic diseases. Methods: Data for 123 patients at Hospital were taken retrospectively. The variables studied were demographic data, vestibular examination, audiometry, and vertigo diagnosis. Results: There were 123 vertigo patients consisting of 42 men and 81 women. The average age of the patients was 48.46 years. Most patients were diagnosed with other peripheral vertigo 37.40%, then unspecified disorder of vestibular function 15.45%, BPPV 14.63%, Meniere’s disease 8.94%, dizziness 8.94%, central vertigo 8.13%, and vestibular neuritis 6.50%. The types of hearing loss were Conductive Hearing loss (CHL) 6.10%, Sensorineural Hearing Loss (SNHL) 21.54%, and Mixed Hearing Loss (MHL) 11.38%. Location of hearing loss was unilateral 23.58% and bilateral 26.83%. Mean Pure Tone Average (PTA) of vertigo with hearing loss was 52.54 dB in right ear and 55.96 dB in left ear. Conclusion: Most patients were diagnosed with other peripheral vertigo is female. They had normal hearing. The most common type of hearing loss was SNHL with a mean PTA of 52.54 dB in right ear and 55.96 dB in left ear. Mean PTA in vertigo patients with normal hearing was 18.29 ± 4.24 dB in right ear and 17.55 ± 4.13 dB in left ear, while mean PTA in vertigo with hearing loss was 52.54 ± 29.93 dB in right ear and 55.96 ± 28.01 in left ear.

Introduction: The prevalence of hearing loss ranges from 1 to 3 per 1000 live births and 2-4 per 100 high-risk babies as in low birth weight babies. Hearing screening in all newborns has not been fully implemented at Dr. Soetomo Hospital Surabaya so this research is expected to be the basis for hearing screening in all babies born especially low birth weight babies (LBW). The objective of the study was to predict the effect of low birth weight babies on impaired cochlear function in newborns. Methods: Cross-sectional comparative study was conducted from September 2018 to March 2019 in Dr. Soetomo Hospital. The subjects of this study were infants aged 2-28 days old in the nursery. Distortion Product Otoacoustic Emissions (DPOAE) was used to examineselected babies who fulfilled the selection criteria. Results: Twenty babies (≥2500 gram) and 20 babies (<2500 grams) were selected in this study. The proportion of cochlear dysfunction in infants with birth weights <1500 grams and 1500-2500 grams in Dr. Soetomo Hospital Surabaya was 50% and 35.7%. In multivariate analysis birth weight <1500 and birth weight 1500-2499 had a risk respectively 2.06 (95% Confidence Interval 0.08-48.26 P = 0.653) and 1.20 (95% Confidence Interval 0.07-20.34 P = 0.899) respectively. The proportion of cochlear dysfuntion in babies with birth weight ≥2500 grams is the highest which is 65 %. Conclusion: In this study Low Birth Weight Babies have a risk of 35.7 % of impaired cochlear function We would recommend DPOAE as screening method in all babies at Dr. Soetomo Hospital, Surabaya which is continued by second screening examination within one month on high risk babies and failed first test.