Aims: This study is focused on the potential of Bacillus cereus ATCC 14579 to degrade natural rubber (NR) latex film and NR latex film added with Metroxylan sagu pith waste (NR/TSPW latex film). Methodology and results: Bacillus cereus is proved capable to utilise rubber as main source of carbon and energy. The biodegradation studies were analyzed by growth profile, weight loss, protein content and tensile strength test. Fourier Transform Infrared Spectroscopy (FTIR) test was applied to confirm the biodegradation process. In shake cultures, OD of culture increased by 19.2% from the initial inoculum after 14th cultivation days with NR/TSPW latex film. An increase in protein content up to 0.037 mg/g with 12.377% weight loss of film was obtained after biodegradation. Tensile test result shows tensile strength and elongation break are decreased by 10.203%. Conclusion, significance and impact of study The demand of rubber products is increasing time to time. Due to the high consumable and disposable of rubber latex products, hence remain inert to degradation and leading to their accumulation in the environment. An attempt to combine Natural Rubber (NR) latex system with other degrading materials have been made to facilitate biodegradation process. Thus, Bacillus cereus ATCC 14579 has potency to provide a biotechnological solution to the waste rubber disposal problem.