Oxidative stress involved in various pathological conditions. Plants have been proven to act as a natural exogenousantioxidant. The aim of this research is to investigate the protective effects of Etlingera coccinea leaves aqueous extracton autoxidation-induced ox brain homogenate. The brain homogenate was divided into 7 groups: control group withPBS solution, positive control group with 100 μg/ml ascorbic acid, test group with 25, 50, 100, 200 and 400 μg/ml of E.coccinea. The antioxidant potential of E. coccinea aqueous extract has been evaluated by antioxidant capacity assaysuch as Total phenolic content (TPC), radical scavenging assay (DPPH) and ferric reducing antioxidant power (FRAP).Malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione (GSH) were also measured at 0 hr and 1 hr after37°C water bath incubation to determine the antioxidant status and oxidative damage. TPC assay showed (4.85 ± 0.28)mg GAE/g of dry weight of E. coccinea leaves. Based on DPPH and FRAP assay, E. coccinea aqueous extract showed adose-dependent antioxidant activity. MDA level in the 50 μg/ml E. coccinea was significantly lower compared to the othergroups (p < 0.05). The SOD activity was significantly increase in 400 μg/ml E. coccinea (p < 0.05) compared to othergroups. E. coccinea at the dose of 25 μg/ml and 50 μg/ml showed significant increase in GSH level compared to othergroups (p < 0.05). In conclusion, 25 μg/ml and 50 μg/ml of E. coccinea leave aqueous extracts exhibited a potentialprotective effect on autoxidation-induced ox brain h