Abstract Methicillin-resistant Staphylococcus aureus (MRSA) causes nosocomial and community infections and is a global health concern. This study evaluated the antibacterial activity of acetone extract from Canarium odontophyllum leaves against MRSA ATCC 33591 and Mu50 strains. The MIC/MBC ratio, determined using the broth micro dilution method, revealed the bacteriostatic effect of the extract against both strains. Time-kill assay against the Mu50 strain showed that the extract inhibited the growth of MRSA at low concentration but exhibited a concentration-dependent bacterial killing effect at 4× MIC. These findings confirm that an acetone extract from C. odontophyllum leaves inhibited growth of MRSA at low concentrations and could be utilised as an alternative anti-MRSA agent.