1.Study on Extraction Technology Optimization and Structural Characterization and of Fritillaria pallidiflora Poly- saccharide
Abuduwaili AYITUXUN ; Rouzi PAERHATI ; Yanhua GAO ; Mutailifu PAIHEERDING ; Nuerxiati REHEBATI ; Huixia NING ; Yili ABULIMITI
China Pharmacy 2020;31(3):276-281
OBJECTIVE:To optimize the extractio n technology of Fritillaria pallidiflora polysaccharides(FPSP),and to study its structure. METHODS :Using the yield of FPSP as response value ,Box-Behnken design-response surface methodology was adopted to optimize solid-liquid ratio ,extraction temperature and extraction time of FPSP extraction technology. Structural properties of FPSP was characterized by UV spectrum ,FTIR,GC-MS,Congo red staining ,SEM,XRD and thermogravimetric analysis. RESULTS:The optimal technology parameters of FPSP were solid-liquid ratio of 1∶28(g/mL),extraction temperature of 94 ℃, extraction time of 2.5 h;the yield of FPSP was 16.25%(n=3),the error of which to theoretical yield (16.58%)was 0.33%. FPSP contained xylose ,glucose and galactose with a molar ratio of 1∶58.02∶0.73,and trace amount of mannose ;there was a weak absorption peak near the wavelength of 280 nm;belonged to α-configuration pyranopolysaccharide. FPSP was in triple-helical structure. The surface of FPSP was a network structure formed by irregular particles. FPSP had both crystalline and amorphous structures. FPSP had good thermostability. CONCLUSIONS :The optimized extraction technology of FPSP is reasonable ,and has high extraction yield. The research might provide reference for the further development and utilization of F. pallidiflora .