No abstract available.
DNA* ; Nucleolus Organizer Region*

No abstract available.
Nucleolus Organizer Region* ; Proliferating Cell Nuclear Antigen*

No abstract available.
Nucleolus Organizer Region* ; Proliferating Cell Nuclear Antigen*

No abstract available.
Animals ; Mucous Membrane* ; Nucleolus Organizer Region* ; Rats*

No abstract available.
Carcinoma, Squamous Cell* ; Cervix Uteri* ; Female ; Nucleolus Organizer Region*

BACKGROUND AND OBJECTIVES: The Nucleolar organizer regions (NORs) are one of markers of cellular proliferation. Because the NORs can be visualized by a silver staining technique, the NORs are called the argyrophilic nucleolar organizer regions (AgNORs). The expression patterns of proliferative markers have been reported in the cholesteatoma, but the AgNORs have not been studied in the cholesteatoma. We investigated the proliferative activities of the cholesteatoma by the AgNORs and the usefulness of the AgNORs as a proliferative index in the cholesteatoma. MATERIALS AND METHODS: We assessed 5 postauricular skin samples and 20 cholesteatoma specimens by the numbers of the total AgNORs and the large AgNORs (large AgNOR means a diameter of over 6 nm) in high power fields and each cell. And the total areas of the AgNORs in high power fields (HPF) were calculated. RESULTS: The numbers of the large AgNORs in HPF, the numbers of AgNORs in each cell and the total areas of the AgNORs in HPF of the cholesteatoma were higher than those of the controls (p<0.05). In the cholesteatoma, the numbers of the large AgNORs and the total areas of the AgNORs in HPF were the highest in the keratinizing squamous epithelium of thick portion followed by the non-keratinizing squamous epithelium, and the keratinizing epithelium of thin portion. The numbers of the large AgNORs in each cell of the basal and superficial layers were the highest in the thick keratinizing squamous epithelium. In the suprabasal layer, the non-keratinizing squamous epithelium showed higher numbers of the large AgNORs but showed no statistical significance. CONCLUSION: 1) The proliferative capacity of the epithelium of cholesteatoma is reactive proliferative status. 2) The proliferative activity is varied with the differentiation status of the squamous epithelium in the cholesteatoma.
Cell Proliferation ; Cholesteatoma* ; Epithelium* ; Nucleolus Organizer Region* ; Silver Staining ; Skin

BACKGROUND: Nucleolar organizer regions (NORs) have recently attracted much attention because of claims that their frequency within nuclei is significantly higher in malignant cells than in normal, reactive, or benign neoplastic cells. OBJECTIVE: The purpose of this paper is to analyze a method allowing selection of the best morphometric criterion for quantifying AgNOR proteins under conventional observation conditions by light microscopy. METHOD: We tried to investigate the various parameters including NORs counting in cutaneous tumors using image analysis system. RESULTS: There were significant differences in mean nucleus area per a AgNOR, nucleus area between the benign and potentially malignant group. But the conventional counting of AgNORs is not able to differentiate between the two groups. We could discriminate squamous cell carcinoma from Bowen's disease using parameters of mean ratio of AgNORs area per nucleus area, mean ratio of greatest AgNORs area per nucleus area, coefficient of variation (C V) of nucleus area, and mean area of largest AgNORs. In squamous cell carcinoma and keratoacanthoma, C V of nucleus area has shown a significant difference. CONCLUSION: Study of AgNORs using image analysis system is a useful tool for the diagnosis of cutaneous tumors.
Bowen's Disease ; Carcinoma, Squamous Cell ; Diagnosis ; Keratoacanthoma ; Methods ; Microscopy ; Nucleolus Organizer Region* ; Skin*

Paraffin-embedded surgical specimens from 27 human astrocytic tumors(7 astrocytomas, 10 anaplastic astrocytomas and 10 glioblastomas) were analyzed immunohistochemically for the presence of p53 protein and proliferation markers of proliferating cell nuclear antigen(PCNA), Ki-67 and agyrophilic nucleolar organizer regions (AgNORs). Approximately 33% of total cases were p53-protein positive. The p53-protein positive nuclei were revealed in 5 cases(50%) of glioblastomas, 4 cases(40%) of anaplastic astocytomas. None of the astrocytomas including 2 pilocytic tumors was p53-protein positive. There were no differences between histological types and p53-protein expression(p=0.0593), however, the more malignant histological features appear to be reflected by a greater incidence of p53 accumulation. In comparative evaluation of p53-protein expression and proliferation indices of PCNA, Ki-67 and AgNORs statistical analysis revealed significant correlation between p53 protein expression and Ki-67 labeling indices only(p=0.0177). p53-protein positive astrocytic tumors showed higher Ki-67 labeling index(10.8+/-6.9%) compared to p53-protein negative tumors(5.3+/-4.3%). In conclusion the malignant histological feature of astrocytic tumors may be associated with p53-protein expression, and among proliferation indices Ki-67 labeling index is correlated with p53-protein expression.
Astrocytoma ; Glioblastoma ; Humans ; Incidence ; Nucleolus Organizer Region ; Proliferating Cell Nuclear Antigen

Nucleolar organizer regions (NORs) are loops of DNA which occur in the nucleoli of cells and which possess ribosomal RNA (rRNA) genes. The numbers and/or configurations of NORs have been thought to be related to cellular activities. To assess the applicability of NORs associated protein (Ag-NORs) in the field of diagnostic histopathology, a silver staining was done in paraffin sections of malignant lymphomas, tonsils and reactive lymph nodes and the numbers of Ag-NORs in the nuclei of low-grade and those of high-grade lymphomas were compared. A significant difference was found between the numbers of Ag-NORs in the nuclei of low-grade lymphoma (a mean of 1.3 per nucleus) and those of high-grade lymphomas (a mean of 4.2 to 8.3 per nucleus). The Ag-NORs were often observed in nuclei in areas where nucleoli themselves were not visible in H and E stain. It is suggested that this method would be of great value in the field of tumor histopathology.
Histological Techniques ; Humans ; Korea ; Lymphoma, Non-Hodgkin/ethnology/*pathology ; Nucleolus Organizer Region/*pathology ; *Silver ; *Staining and Labeling

Nucleolar organizer regions (NORs) are loops of DNA which occur in the nucleoli of cells and which possess ribosomal RNA (rRNA) genes. The numbers and/or configurations of NORs have been thought to be related to cellular activities. To assess the applicability of NORs associated protein (Ag-NORs) in the field of diagnostic histopathology, a silver staining was done in paraffin sections of malignant lymphomas, tonsils and reactive lymph nodes and the numbers of Ag-NORs in the nuclei of low-grade and those of high-grade lymphomas were compared. A significant difference was found between the numbers of Ag-NORs in the nuclei of low-grade lymphoma (a mean of 1.3 per nucleus) and those of high-grade lymphomas (a mean of 4.2 to 8.3 per nucleus). The Ag-NORs were often observed in nuclei in areas where nucleoli themselves were not visible in H and E stain. It is suggested that this method would be of great value in the field of tumor histopathology.
Histological Techniques ; Humans ; Korea ; Lymphoma, Non-Hodgkin/ethnology/*pathology ; Nucleolus Organizer Region/*pathology ; *Silver ; *Staining and Labeling