1.Primarily introduction of polymerase chain reaction (PCR)
Journal of Practical Medicine 2002;435(11):36-39
The PCR has been lately established but applied and developed rapidly so that it is a vital method for biomolecular researchers. The scientific development helped this technique becoming simplly more and was and its application is widely
Polymerase Chain Reaction
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Nuclear Magnetic Resonance, Biomolecular
2.Preliminary study on chemical constituents seperated from Cayratia japonica.
Chuan-wen CUI ; Cui-ling SUN ; Quan-cheng CHEN ; Xiu-hong ZOU ; Xue-min HUANG ; Hai-feng CHEN
China Journal of Chinese Materia Medica 2012;37(19):2906-2909
OBJECTIVETo study effective active constituents of Cayratia japonica,a genuine herbal medicine from Fujian.
METHODSuch chromatographic methods as Macroporous, Sephadex LH-20, ODS and normal phase silica gel column chromatography were adopted to separate the chemical components of C. japonica.
RESULTThirteen compounds were obtained, and their structures were identified by analyzing multiple spectral data as luteolin(1), apigenin(2), triethyl citrate-(3), 3-formylindole(4), esculetin(5), bis(2-ethylhexyl)-phthalate(6), calendin(7), ethyl-trans-3,4-dihydr-oxycinnamate(8), luteolin7-O-D-glucoside(9),5-hydroxy-3,4-dimethyl-5-pentyl-2(5H-furanone(10),ethyl-3,4-dihydroxybenzoate(11), eriodictyol(12) and daucosterol(13).
CONCLUSIONAmong them, compounds 3-8 and 10-12 were separated from the plant for the first time.
Nuclear Magnetic Resonance, Biomolecular ; Plants, Medicinal ; chemistry ; Vitaceae ; chemistry
3.Flavonoids of Erigeron canadensis.
Shuai SHAO ; Ming-ming YANG ; Sheng-nan BI ; Zhi-qiang WAN
China Journal of Chinese Materia Medica 2012;37(19):2902-2905
OBJECTIVETo study the flavonoids of Erigeron canadensis.
METHODThe constituents of EtOH extraction from the whole plant of E. canadensis were isolated and purified by repeated column chromatography. These compounds were identified by their physical and spectral data.
RESULTTwelve flavonoids were isolated and identified as quercetin-7-O-beta-D-galactopyranoside(1),quercetin(2), luteolin(3), apigenin(4),5,7,4'-trihydroxy-3'-methoxy flavone(5), quercetin-3-alpha-rhamnopyranoside(6), quercetin-3-O-beta-D-glucopyranoside(7), apigenin-7-O-beta-D-glucopyranoside(8), luteolin-7-O-beta-D-glucuronide methyl ester(9),4'-hydroxy baicalein-7-O-beta-D-glucopyranoside(10),baicalein(11),rutin(12).
CONCLUSIONCompound 1 was isolated from the Compositae family for the first time. Compound 5 and 9 were firstly isolated from the genus Erigeron. Compound 3,4,7,8 and 11 were isolated from E. canadensis for the first time.
Conyza ; chemistry ; Flavonoids ; chemistry ; isolation & purification ; Nuclear Magnetic Resonance, Biomolecular
4.Studies on chemical constituents of Uvaria tonkinensis var. subglabra.
An LIU ; Lizhen XU ; Zhongmei ZOU
China Journal of Chinese Materia Medica 2009;34(5):567-570
OBJECTIVETo isolate and identify chemical constituents of Uvaria tonkinensis var. subglabra.
METHODThe column chromatographic techniques were applied to isolate constituents, and their structures were elucidated by means of spectral data analysis including 1D and 2D NMR, IR and MS techniques.
RESULTSeven compounds were isolated and identified as subglain C (1), beta-senepoxide (2) , 2, 4-dioxohexahydro-1, 3-diazepin (3), kaempferol-3, 7-di-O-alpha-L-rhamnoside (4), anolobine (5), (-)-lyoniresinol (6) and schizandriside (7).
CONCLUSIONCompound 1 was the new natural product; compounds 2-7 were isolated for the first time from U. tonkinensis var. subglabra.
Chromatography ; Drugs, Chinese Herbal ; chemistry ; Mass Spectrometry ; Nuclear Magnetic Resonance, Biomolecular ; Uvaria ; chemistry
5.New tannin-related compounds from Tagetes erecta.
Xianli ZHOU ; Shuai HUANG ; Xiaoli ZHOU ; Jie WENG ; Cuijuan WANG
China Journal of Chinese Materia Medica 2012;37(3):315-318
A mixture of two new anomers: 3,4-di-O-[syringate]-alpha-D-glucopyranose (1) and 3,4-di-O-[syringate]-beta-D-glucopyranose (2), together with syringic acid (3), were isolated from the alcohol extract of Tagetes erecta. The structures of these compounds were elucidated by various spectroscopic methods, including intensive 1D, 2D NMR and HR-ESI-MS studies. The structural assignment was further supported by HPLC-ESI-MS with compound 1 at t(R) = 3.22 min (m/z 538.9 [M-H](-)) and compound 2 at 3.25 min (m/z 538.9 [M-H](-)) in the mixture.
Flowers
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chemistry
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Nuclear Magnetic Resonance, Biomolecular
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Plant Extracts
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chemistry
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Tagetes
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chemistry
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Tannins
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analysis
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chemistry
7.Study on Urinary Metabolic Profile in Rats with Deep Venous Thrombosis Based on Pattern Recognition.
Jie CAO ; Xiao Ge LÜ ; Yu LI ; Qian Qian JIN ; Xiao Yun CHU ; Ying Yuan WANG ; Jun Hong SUN
Journal of Forensic Medicine 2018;34(3):228-232
OBJECTIVES:
To study the urinary metabolic profile in rats with deep venous thrombosis (DVT) based on metabolomics and to screen out small molecular biomarkers for the diagnosis and forensic identification of DVT.
METHODS:
Inferior vena cava of rats was ligated to construct DVT models. The rats were randomly divided into three groups: DVT, sham, and control groups, 10 in each group. The urine of DVT and sham rats was collected during 24 hours in the metabolic cage at 48 hours after operating, meanwhile, 24 hours urine was collected in control group. The metabolic profile was analyzed by nuclear magnetic resonance. SIMCA-P 14.1 software was used for pattern recognition. The variable importance in projection (VIP) value from orthogonal PLS-DA (OPLS-DA) model combined with Mann-Whitney U test were used to search the different metabolites in the urine.
RESULTS:
The metabolic profiles of urine from DVT, sham, and control groups had significant differences. The DVT, sham, and control groups could be distinguished by the partial least squares method-discriminant analysis (PLS-DA) model. Compared with the urine of the rats in control groups, the levels of leucine, glutamine, creatine, creatinine and sucrose in the urine of DVT rats were up-regulated, and the levels of 3-hydroxybutyrate, lactate, acetone, α-oxoglutarate, citrate and hippurate were down-regulated.
CONCLUSIONS
The different metabolites in the urine of DVT rats are expected to become its candidate biomarkers. The results can provide a research basis for the diagnosis, treatment and forensic identification of DVT.
Animals
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Biomarkers/blood*
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Discriminant Analysis
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Humans
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Magnetic Resonance Spectroscopy/methods*
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Metabolome
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Metabolomics/methods*
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Nuclear Magnetic Resonance, Biomolecular/methods*
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Rats
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Rats, Sprague-Dawley
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Urine/chemistry*
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Venous Thrombosis/urine*
8.Chemical constituents in Thunbergia from Africa.
Feng-Jie HUNG ; Jian-Xiao SONG ; Jia-Jian LIU ; Ai-Hu ZHAO ; Wei JIA
China Journal of Chinese Materia Medica 2013;38(8):1183-1187
OBJECTIVETo investigate the chemical compounds from the ethanol extract with inhibitory effects against aldose reductase from Thunbergia.
METHODGuided by anti-aldose reductase assay, compounds from the bioactive fraction (ethyl acetate extract) were separated and purified by various chromatographic methods including silica gel, Sephadex LH-20, and reversed-phase HPLC. Their structures were indentified based on analysis of the spectroscopic data including 1D and 2D NMR data.
RESULTEight compounds were obtained and identified as 8-hydroxy-8-methyl-9-methene-cyclopentane [7,11] -1,4, 6-trihydroxy-tetrahydronaphthalene-12-one, named as thunbergia A (1), 3,4-dihydro-4,5,8-trihydroxy-2-(3-methyl-2-butenyl) naphtha[2,3-b] oxiren-1(2H)-one (2), 8-(beta-gluco pyranosyloxy)-3,4-dihydro-2-(3-methyl-2-butenyl)naphtha [2,3-b] oxiren-1(2H)-one (3), galangin (4), quercetin (5), luteolin (6), 5,6,3',4'-tetrahydroxy -3,7-dimethoxy-flavone (7) and upeol (8).
CONCLUSIONThunbergia A was a new derivative of tetrahydronaphthalene, and compounds 2 and 3 were separated from the genus Thunbergia for the first time.
Acanthaceae ; chemistry ; Aldehyde Reductase ; antagonists & inhibitors ; Animals ; Nuclear Magnetic Resonance, Biomolecular ; Plant Extracts ; chemistry ; isolation & purification ; pharmacology ; Plant Roots ; chemistry ; Rats
9.Impact of experimental conditions on the results of NMR-based metabononmics study.
Yi LI ; Jinglin ZHOU ; Wei LI
Journal of Biomedical Engineering 2010;27(2):241-244
In the process of preparing and analyzing the metabonomics samples, there are lots of influential factors such as sample individual differences, environmental factors and human factors. Here we report a study on 3 healthy male SD rats of different samples carried out by different operators (n=4). It was a two-way experiment designed to explore the impact factors and lay the foundation for future experiments. The cluster analysis was employed in comparing 12 samples. The results showed that the differences of rats between the sample groups had a certain influence on the metabonomics data, and there was relatively little variability between the 4 operators. That is to say, whilst the sample are rather different from one another, a given sample is generally quite similar to itself in metabonomics study.
Animals
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Blood Chemical Analysis
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methods
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Cluster Analysis
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Humans
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Male
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Metabolomics
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methods
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Nuclear Magnetic Resonance, Biomolecular
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methods
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Rats
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Rats, Sprague-Dawley
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Reproducibility of Results
10.Study on triterpenes from Solanum torvum.
Hongbo ZHOU ; Feng WANG ; Zhijian FANG
China Journal of Chinese Materia Medica 2011;36(15):2096-2098
OBJECTIVETo study on the chemical constituents of Solanum torvum.
METHODThe aerial parts of S. torvum swartz were extracted with 95% alcohol and the constituents were isolated and purified by chromatographic silica gel and Sephadex LH-20. Their structures were determined by NMR and MS spectral analysis.
RESULTSix triterpenes were isolated and identified as 3beta-acetyloleanolic acid (1), 3-O-acetyl-11alpha, 12alpha-epoxy-oleanan-28, 13beta-olide (2), oleanolic acid (3), ursolic acid (4), 2alpha-hydroxy-oleanolic acid (5), 2alpha, 3beta-dihydroxyursolic acid (6).
CONCLUSIONAll compounds were obtained from this plant for the first time.
Drugs, Chinese Herbal ; chemistry ; isolation & purification ; Gas Chromatography-Mass Spectrometry ; Nuclear Magnetic Resonance, Biomolecular ; Plants, Medicinal ; chemistry ; Solanum ; chemistry ; Triterpenes ; chemistry ; isolation & purification