2.Meta analysis of the relationship between human papilloma virus and nasal inverted papilloma.
Wu XIAO ; Shanfeng LIU ; Liyin WANG ; Huimin LI ; Wenwei WU ; Zhenhai WANG
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2013;27(11):572-576
OBJECTIVE:
To evaluate the relationship between human papilloma virus (HPV) infection and the occurrence, recurrence and malignant transformation of nasal inverted papilloma (NIP).
METHOD:
With comprehensive retrieval of related literature that had been published in databases included Pubmed (1990--2011), Cochrane Library, CNKI (1979-2011), VIP (1989-2011), CBM (1990-2011) and WANFANG Meta-analysis software Rev-Man 5.0 was used to analyze the raw data and to calculate the value of combined odd ratio (OR) and 95% confidence interval (CI).
RESULT:
According to Meta-analysis, the occurrence group between HPV and NIP, the OR was 34.44 and 95% CI was 12.96-91.56; the recurrence group, the OR was 3.66 and 95% CI was 1.77-7.56, to the high-risk HPV, the OR was 1.94 and 95% CI was 0.30-12.58; the malignant transformation group, the OR was 1.79 and 95% CI was 0.94-3.40 to the high-risk HPV, the OR was 49.35 and 95% (CI was 0.45-11.23.
CONCLUSION
HPV may play an important role in the occurrence and recurrence of NIP and high-risk HPV was closely related to the progress of NIP.
DNA, Viral
;
Humans
;
Nose Neoplasms
;
virology
;
Papilloma, Inverted
;
virology
;
Papillomaviridae
;
Papillomavirus Infections
;
pathology
3.Relationship between prognosis of nasal inverted papilloma and human papillomavirus types.
Shan-shan LU ; Ren ZOU ; Ji-wei XU
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2005;40(3):195-198
OBJECTIVETo detected HPV human papillomavirus DNA and HPV types in NIP nasal inverted papilloma, to inquire into the infection of HPV in the pathogenesis of NIP, and the relationship of HPV with the prognosis of NIP.
METHODSTwenty-eight cases of NIP were divided into 3 groups: no recurrence group (group 1), recurrence group (group 2), squamous cell carcinoma (SCC) arising in NIP group (malignancy group, group 3). Ten cases of benign nasal polyps were used as control group. HPV-DNA types of 6, 11, 16, 18 and general type were detected by polymerase chain reaction (PCR).
RESULTSTotal positive rate of HPV in NIP was 75% (21/28). The positive rate of group 1 was 42% (5/12), all with single and low risk HPV type infection (4 with HPV6 and 1 with HPV11). Thirteen cases of recurrence and 3 cases of malignancy were discovered to have HPV-DNA, in group 2, the majority were HPV6 and HPV11, 4 cases with double infection;in group 3, the majority were HPV16 and HPV18, and 2 cases with double infection.
CONCLUSIONSThe occurrence of NIP was related with HPV infection. To detected HPV and its subtypes can show the cases easily to have recurrence or malignant change. For the cases with HPV positive, double infection and infected with high risk types should be closely followed-up.
Case-Control Studies ; Humans ; Nose Neoplasms ; diagnosis ; virology ; Papilloma, Inverted ; diagnosis ; virology ; Papillomaviridae ; classification ; genetics ; Papillomavirus Infections ; diagnosis ; virology ; Paranasal Sinus Neoplasms ; diagnosis ; virology ; Prognosis
4.Relationship between nasal inverted papilloma and human papillomavirus subtypes.
Pu SUN ; Xiao-ping CHEN ; Fei PEI ; Rui-xia MA ; Yi ZHANG ; Qun CHEN ; Wei-gang DONG ; Wei-xiang CHEN ; Hui-li HUANG
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2010;45(4):310-313
OBJECTIVETo study the distribution of human papillomavirus (HPV) subtypes in nasal inverted papilloma (NIP), and to evaluate the relationship between HPV and NIP.
METHODSTwenty-one HPV subtypes were detected in paraffin-embedded tissues of 101 cases of NIP by flow through hybridization and gene chip (HybriMax), 24 cases of normal nasal mucosa were used as controls.
RESULTSHPV positive rates of NIP were 64.36% (65/101). Benign NIP group, NIP with atypical hyperplasia group, NIP with cancerous group of HPV positive rates were 59.7% (46/77), 81.8% (18/22) and 50% (1/2) respectively. The control group was negative (0/24). The comparison between NIP group and control group was statistically significant (chi(2) = 32.178, P < 0.05). Benign NIP group and NIP with atypical hyperplasia group were compared, but no statistically significance (chi(2) = 3.649, P = 0.056) was found. The constituent ratio of benign NIP group and NIP with atypical hyperplasia group in high, low-risk HPV subtypes infections was compared, a statistically significance (chi(2) = 10.412, P < 0.05) was found.
CONCLUSIONSThe occurrence of NIP was related with HPV infection. High-risk HPV subtype infections or multiple infections will prompt benign NIP to NIP with atypical hyperplasia. Understanding the distribution of HPV subtypes in the NIP is helpful to predict the clinical behavior.
Adult ; Aged ; Case-Control Studies ; Female ; Humans ; Male ; Middle Aged ; Nose Neoplasms ; pathology ; virology ; Papilloma, Inverted ; pathology ; virology ; Papillomaviridae ; classification ; Papillomavirus Infections ; pathology
5.The study on the relationship between human papillomavirus infection and pathogenesis of nasal inverted papilloma and its malignant transformation.
Zhenhua ZHONG ; Aihui YAN ; Feifei JIANG ; Hongquan WEI ; Xuejun JIANG
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2010;24(5):209-211
OBJECTIVE:
To investigate the relationship between human papillomavirus (HPV) infection and pathogenesis of nasal inverted papilloma (NIP) and its malignant transformation.
METHOD:
Fifty-seven cases of NIP were divided into 2 groups: benign group, squamous cell carcinoma (SCC) arising in NIP group (malignancy group). HPV-DNA types of 6, 11, 16, 18 were detected by polymerase chain reaction (PCR) in 57 cases of NIP. Thirty cases of nasal polyps were control.
RESULT:
Total positive rate of HPV in NIP was 64.9% (37/57). The positive rate of benign group was 60% (27/45), all with single and low risk HPV11 type infection. The positive rate of malignancy group were was 83.3% (10/12), and the majority were HPV16 and HPV18. Five cases had double infection (4 with HPV16 and HPV 18, 1 with HPV11 and HPV18), four had single HPV16 infection, another one had single HPV11 infection. However, HPV-DNA was not detected in any cases of nasal polyps.
CONCLUSION
Infection of HPV-DNA has an important effect in pathogenesis of NIP. Meanwhile, there maybe a close relationship between high risk HPV16 type and HPV18 type and malignant transformation of NIP.
Adult
;
Aged
;
Aged, 80 and over
;
Cell Transformation, Neoplastic
;
pathology
;
Female
;
Humans
;
Male
;
Middle Aged
;
Nose Neoplasms
;
pathology
;
virology
;
Papilloma, Inverted
;
pathology
;
virology
;
Papillomaviridae
;
Papillomavirus Infections
;
pathology
;
Young Adult
6.Sinonasal primary extramedullary solitary plasmacytoma with Epstein-Barr virus infection: report of a case.
Yan-fen FENG ; Qiu-liang WU ; Yong-sheng ZONG ; Qiong SHAO
Chinese Journal of Pathology 2007;36(10):711-712
Antibodies, Monoclonal
;
metabolism
;
CD79 Antigens
;
metabolism
;
Epstein-Barr Virus Infections
;
Herpesvirus 4, Human
;
isolation & purification
;
Humans
;
Immunoglobulin G
;
metabolism
;
Male
;
Middle Aged
;
Nose Neoplasms
;
metabolism
;
pathology
;
therapy
;
virology
;
Paranasal Sinus Neoplasms
;
metabolism
;
pathology
;
therapy
;
virology
;
Plasmacytoma
;
metabolism
;
pathology
;
therapy
;
virology
7.Sample type is vital for diagnosing infection with peste des petits ruminants virus by reverse transcription PCR.
Pam Dachung LUKA ; Chrisostom AYEBAZIBWE ; David SHAMAKI ; Frank Norbert MWIINE ; Joseph ERUME
Journal of Veterinary Science 2012;13(3):323-325
Peste des petits ruminants (PPR) diagnosis from suspected samples from sheep and goats was carried out. Buffy coat, tissues, and oculo-nasal swabs were analyzed using nucleoprotein (NP3/NP4) and fusion protein (F1/F2) gene primers, respectively. Analysis of the sample types and primer set revealed that buffy coat are the best type of samples for PPR diagnosis and the use of two set of primers will increase the number of positives.
Animals
;
DNA Primers/analysis
;
Eye/virology
;
Goat Diseases/blood/*diagnosis/epidemiology/virology
;
Goats
;
Hair/virology
;
Nose/virology
;
Nucleoproteins/analysis
;
Peste-des-Petits-Ruminants/blood/*diagnosis/epidemiology/virology
;
Peste-des-petits-ruminants virus/genetics/*isolation & purification
;
Pigmentation
;
RNA, Viral/genetics/*isolation & purification
;
Reverse Transcriptase Polymerase Chain Reaction/*methods/standards/veterinary
;
Sheep
;
Sheep Diseases/blood/*diagnosis/epidemiology/virology
;
Uganda/epidemiology
8.Prevalence of EBV RNA in sinonasal and Waldeyer's ring lymphomas.
Ju Hie LEE ; Sang Sook LEE ; Jae Hoon PARK ; Youn Wha KIM ; Moon Ho YANG
Journal of Korean Medical Science 1994;9(4):281-288
A high incidence of a T cell phenotype of sinonasal lymphomas in other Asian countries has been associated with a high incidence of Epstein Barr virus (EBV) infection. We analyzed 13 sinonasal and 18 Waldeyer's ring lymphomas for the prevalence of EBV encoded RNA (EBER) using a sensitive and specific in situ hybridization. In addition, we examined the relationship of histologic findings and immunophenotype as well as the location of the lymphomas to the presence of EBV. The EBER was detected in each of 12 sinonasal lymphomas with a T cell immunophenotype. One B cell sinonasal lymphoma was EBER negative. Four of 18 Waldeyer's ring lymphomas were positive for EBER, including two T cell lymphomas. Two of 16 B cell Waldeyer's ring lymphomas were EBER positive. Morphologically, 11 of 20 diffuse large cell lymphomas, 2 diffuse mixed small and large cell lymphomas, 2 of 4 immunoblastic lymphomas and 1 lymphoplasmacytic lymphoma were EBER positive. Four follicular large cell lymphomas were EBER negative. A characteristic angiocentric or angiodestructive pattern was found in most T cell lymphomas and EBER positive cases. These findings indicate that EBV infection is more strongly associated with the T cell immunophenotype, angiocentric pattern and sinonasal location of the lymphoma.
Adult
;
Aged
;
B-Lymphocytes/immunology
;
Female
;
Herpesviridae Infections/*virology
;
Herpesvirus 4, Human/genetics/*isolation & purification
;
Human
;
Immunophenotyping
;
In Situ Hybridization
;
Incidence
;
Lymphoid Tissue/virology
;
Lymphoma, Non-Hodgkin/epidemiology/immunology/mortality/*virology
;
Male
;
Maxillary Neoplasms/epidemiology/immunology/mortality/*virology
;
Middle Age
;
Nasopharyngeal Neoplasms/epidemiology/immunology/mortality/*virology
;
Nose Neoplasms/epidemiology/immunology/mortality/*virology
;
Prevalence
;
RNA, Viral/*analysis
;
Support, Non-U.S. Gov't
;
Survival Rate
;
T-Lymphocytes/immunology
;
Tonsillar Neoplasms/epidemiology/immunology/mortality/*virology
;
Tumor Virus Infections/*virology
9.Detection of the 30 base pair deletion of Epstein-Barr virus latent membrane protein 1 in extranodal nasal type NK/T-cell lymphoma and its prognostic significance.
Sha ZHAO ; Wei-Ping LIU ; Xiao-Ling WANG ; Wen-Yan ZHANG ; Wei JIANG ; Yuan TANG ; Gan-Di LI
Chinese Journal of Pathology 2005;34(11):720-723
OBJECTIVETo compare the detection rate of Epstein-Barr virus latent membrane protein 1 (LMP1) 30 base pair deletion in extranodal nasal type NK/T-cell lymphoma with that in chronic inflammation of nasopharynx and tonsillitis; and to analyze the prognostic significance of LMP1 deletion in extranodal nasal type NK/T-cell lymphoma.
METHODSPolymerase chain reaction was used to detect the deletion of LMP1 in 55 cases of extranodal nasal type NK/T-cell lymphoma and 19 cases of chronic inflammation of nasopharynx and tonsillitis. Follow-up information of 1 to 58-month duration was available in 33 patients.
RESULTSIn all the 55 extranodal nasal type NK/T-cell lymphoma cases studied, 9 cases contained the wide-type or predominantly wide-type LMP1. On the other hand, 46 cases contained the deleted or predominantly deleted LMP1. In the non-lymphoma control group, 16 cases contained the deleted or predominantly deleted LMP1. However, no statistically significant difference was found in the detection rate of 30 base pair deleted LMP1 between extranodal nasal type NK/T-cell lymphoma and control group (P > 0.05). The prognosis of deleted or predominantly deleted LMP1 in extranodal nasal type NK/T-cell lymphoma was worse.
CONCLUSIONThough 30 base pair deletion of Epstein-Barr virus LMP1 may not be an important pathogenetic step in extranodal nasal type NK/T-cell lymphoma, it may play some role in tumor progression.
Adolescent ; Adult ; Aged ; Base Sequence ; Epstein-Barr Virus Infections ; genetics ; virology ; Female ; Follow-Up Studies ; Herpesvirus 4, Human ; genetics ; Humans ; Killer Cells, Natural ; pathology ; virology ; Lymphoma, T-Cell, Peripheral ; genetics ; virology ; Male ; Middle Aged ; Nasopharyngitis ; Nose Neoplasms ; genetics ; virology ; Sequence Deletion ; Survival Rate ; Tonsillitis ; genetics ; virology ; Viral Matrix Proteins ; genetics ; isolation & purification
10.Nasal and pharyngeal non-Hodgkin lymphomas and their relationship with Epstein-Barr virus: a report of 158 cases.
Yan-Jiao HE ; Xin-Shan JIA ; Kazuhisa HASUI ; En-Hua WANG ; An-Guang HE
Chinese Journal of Pathology 2007;36(2):94-97
OBJECTIVETo study the clinical features, immunophenotypes and the significance of Epstein-Barr virus infection in primary nasal and pharyngeal non-Hodgkin's lymphomas in Shenyang.
METHODSOne hundred and fifty eight cases of primary nasal and pharyngeal non-Hodgkin's lymphomas were included in this study. The samples were stained with haematoxylin and eosin for histological examination. Immunohistochemistry studies were performed using monoclonal antibodies, including CD3 for T-lymphocytes, CD20 for B-lymphocytes, and CD56 and CD57 for NK cells. All cases were reclassified according to the new WHO classification of lymphomas (2001). In situ hybridization detection of EBV-encoded small nuclear RNA (EBER-1) was performed in 99 cases.
RESULTSOverall, 101 (63.9%) of the 158 NHL were extranodal NK/T cell lymphomas (nasal type), 23 (14.6%) were nonspecific peripheral T cell lymphomas and the remaining 34 cases (21.5%) were B cell lymphomas. The primary sites of involvement were the nasal cavity (53.2%, 84/158), the tonsil (24.7%, 39/158) and the pharynx (22.1%, 35/158). Among 99 cases studied by EBER-1 in situ hybridization, a positive detection was seen in 70/71 cases (98.6%) of extranodal NK/T cell lymphoma (nasal type), 8/12 cases (66.7%) of T cell lymphoma, and 7/16 cases (43.8%) of B cell lymphoma.
CONCLUSIONSAmong primary nasal and pharyngeal NK lymphomas, extranodal NK/T cell lymphoma (nasal type) is the most common type and is strongly associated with EBV infection. The pathological diagnosis of nasal and pharyngeal lymphomas should take considerations of the anatomic sites and immunophenotypical features.
Adolescent ; Adult ; Aged ; Aged, 80 and over ; CD3 Complex ; metabolism ; CD56 Antigen ; metabolism ; Child ; Female ; Herpesvirus 4, Human ; isolation & purification ; Humans ; Lymphoma, B-Cell ; metabolism ; pathology ; virology ; Lymphoma, Extranodal NK-T-Cell ; metabolism ; pathology ; virology ; Lymphoma, Non-Hodgkin ; metabolism ; pathology ; virology ; Lymphoma, T-Cell, Peripheral ; metabolism ; pathology ; virology ; Male ; Middle Aged ; Nasal Cavity ; Nose Neoplasms ; metabolism ; pathology ; virology ; Pharyngeal Neoplasms ; metabolism ; pathology ; virology ; RNA, Viral ; metabolism ; Tonsillar Neoplasms ; metabolism ; pathology ; virology ; Young Adult