Aims: Rice blast disease caused by Pyricularia oryzae is one of the major biotic diseases of rice in Sarawak, Malaysian Borneo. This study aims to isolate and characterize rice blast fungus obtained from infected leaf collected from four different divisions in Sarawak, viz, Miri, Serian, Sri Aman, and Kuching. Methodology and results: Twelve succeeded isolates were pre-identified as P. oryzae by morphological characteristics of spores, followed by verification through (internal transcribed spacer) ITS sequencing. The isolates were evaluated for morphological characteristics, growth rate and sporulation rate, which were grown on two types of media, (filtered oatmeal agar) FOMA and (potato dextrose agar) PDA. Morphological characterization showed that the colony surface of the different isolates varied from smooth and fluffy to rough and flattened mycelia; some were with the present of concentric rings, and some with aerial mycelia. The growth rate and sporulation rate of each isolate varied based on types of media used. Most of the isolates grew faster on PDA than on FOMA but produced higher number of spores on FOMA as compared to PDA. Conclusion, significance and impact of study: This preliminary study showed that there were variations observed based on morphological and physiological characterization for the different isolates collected in Sarawak, Malaysian Borneo. This study is the first step towards understanding variation in the population of P. oryzae from Sarawak.

Amblyopia is one of the most common causes of visual defi cit in children. Presently, in the Ministry of Health Malaysia hospitals, there is no documented data on the characteristic and profi le of amblyopia cases. This study was conducted to describe the profi le of new amblyopia cases seen by optometrists at the Ministry of Health (MOH) Hospitals. This study was a retrospective and multicenter study including all MOH hospitals with optometry clinics. Clinical record data of amblyopic patients aged 3 to 17 years old who were newly diagnosed between 1st August 2010 to 31st January 2011 and who fulfi lled the inclusion criteria were obtained. Data collected included demography, systemic history, ocular history and optometric fi ndings and diagnosis. Thirty eight MOH hospitals participated and a total of 301 patients were diagnosed with functional amblyopia within the study period. Mean age for these amblyopic patients was 7.70 + 0.16 years old. Boys were the predominant gender (57.1%) and Malay preceded the other races with a 65.4% occurrence. Mild amblyopia was found in 51.5% of the patients, 31.6% were with moderate amblyopia and only 16.9% of patients were severe amblyopia. The underlying amblyogenic causes assessed were ametropia (61.5%), anisometropia (25.2%), strabismus (9.3%) and stimulus deprivation (4.0%). Refractive error was discovered as the most common cause of amblyopia in this study. It is crucial for optometrists to detect this type of visual impairment and undertake an early optometric intervention