No abstract available.
Nontuberculous Mycobacteria*

No abstract available.
Korea* ; Nontuberculous Mycobacteria*

BACKGROUND: To examine the recovery rate of nontuberculous mycobacteria (NTM) from respiratory specimens and the clinical course of NTM pulmonary disease at a 700-bed secondary hospital. METHODS: This study analyzed the results of 843 acid-fast bacilli (AFB) culture-positive respiratory specimens from 650 subjects collected between May 2003 and April 2008. In addition, the clinical course of NTM pulmonary disease, diagnosed using criteria established by the American Thoracic Society, was examined. RESULTS: There were 67 (7.9%) NTM isolates recovered from 52 (8.0%) subjects. Among the 535 AFB smear-positive specimens, 34 (6.3%) NTM isolates were recovered. There were 33 (10.7%) NTM isolates were recovered from 308 AFB smear-negative specimens. Of 52 subjects with isolated NTM, M. intracellulare was the most common species at 73.1% (n=33), followed by M. kansassi (n=7), M. abscessus (n=2), M. fortuitum (n=2), and M. avium (n=1). Sixteen (30.8%) patients had NTM pulmonary disease and the most common causative organism was M. intracellulare (n=14, 87.5%). Of these, 6 cases attained negative conversion in culture, 4 cases failed to attain negative conversion because of poor cooperation or expiration from complicated underlying lung disease, and 5 cases were transferred to a higher-grade hospital. CONCLUSION: The recovery rate of NTM from respiratory specimens was relatively low and the most common species was M. intracellulare. Patients with NTM pulmonary disease showed variable clinical outcomes.
Humans ; Lung Diseases ; Nontuberculous Mycobacteria

Infections due to Nontuberculous Mycobacteria (NTM) have been recognized increasingly worldwide. We report 10 cases of nontuberculous mycobacteria isolated from sputa, being eight cases of M. szulgai, one of M. gordonae, and one of M. abscessus. All but one M. abscessus-isolating case was developed in the same period in one episode. Therefore, it is likely to be contaminated. NTM is a possible pathogen and infections due to NTM are clinically important. Thus, correct identification and determination of clinical significance should be verified.
Gordonia Bacterium ; Nontuberculous Mycobacteria* ; Sputum*

OBJECTIVES: This study was performed to investigate the prevalence of nontuberculous mycobacteria (NTM) species and to determine the clinical significance of NTM isolates. METHODS: From January 2003 to July 2011, NTMs were identified using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) or reverse blot hybridization assay (REBA). And pulmonary NTM infection was diagnosed by clinical history, underlying disease, radiological and microbiological findings according to the diagnostic criteria of 2007 American Thoracic Society (ATS). RESULTS: Of the 697 AFB culture-positive specimens, NTM was detected in 149 (21.4%) specimens. Among 154 NTM isolates from 149 specimens, M. avium-intracellulare complex (MAC) (48.1%) was the most frequently isolated organisms followed by M. abscessus (13.6%), M. gordonae (9.1%), M. kansasii (8.4%), M. szulgai (3.9%), M. fortuitum complex (3.3%), M. scrofulaceum (2.0%), M. malmoense (1.3%), M. chelonae (1.3%), M. marinum (1.3%), M. genavense (1.3%), M. lentiflavum (1.3%) and M. mucogenicum (0.6%). Among 147NTM isolates from 142 respiratory specimens, 54 NTM isolates (36.7%) were causative organisms in NTM pulmonary infection. CONCLUSIONS: The isolation rate of NTM was 21.4% in clinical specimen, and in some cases NTM species results in pulmonary NTM infection. Because the treatment of pulmonary NTM infection depends on the infecting species, accurate identification and clinical significance of NTM are required for adequate treatment.
Chimera ; Gordonia Bacterium ; Nontuberculous Mycobacteria ; Prevalence

No abstract available.
Acupuncture* ; Mycobacterium chelonae* ; Mycobacterium* ; Nontuberculous Mycobacteria

Vertebral osteomyelitis caused by nontuberculous mycobacteria (NTM) is rarely reported, especially in an immunocompetent host. NTM are usually not susceptible in vitro to antituberculous drugs, and appropriate antimicrobial therapy for treatment of NTM infection is based on susceptibility results, which vary between different NTM species; therefore, treatment of vertebral osteomyelitis caused by NTM is challenging. We report on the first case of vertebral osteomyelitis caused by M. abscessus in an otherwise healthy individual, confirmed by cultures of bone tissue obtained during surgery. Clinical cure was achieved with a combination of antimicrobial therapy and surgery. We also review previous reports of vertebral osteomyelitis caused by NTM.
Bone and Bones ; Humans ; Mycobacterium ; Nontuberculous Mycobacteria ; Osteomyelitis

Humans ; Child ; Nontuberculous Mycobacteria ; Lung Diseases

Mycobacterium shinjukuense is a novel species of nontuberculous mycobacteria (NTM) that was first reported in Japan in 2011. It is a slow-growing NTM pathogen that can cause chronic pulmonary infections. There are only a few reported cases of M. shinjukuense infections, all of which are from Japan. We reported a case of chronic lung disease caused by M. shinjukuense. The organism was identified by 16S rRNA, rpoB, and hsp65 gene sequencing. To the best of our knowledge, this was the first confirmed case of lung disease caused by M. shinjukuense outside of Japan.
Bronchiectasis ; Japan ; Korea* ; Lung Diseases* ; Lung* ; Mycobacterium* ; Nontuberculous Mycobacteria

BACKGROUND: The purpose of this study was to evaluate the usefulness of direct PCR for a rapid detection of Mycobacterium tuberculosis (MTB), the differentiation of MTB from nontuberculous mycobacteria (NTM), and the identification of NTM species in acid-fast bacilli (AFB) smear-positive specimens. METHODS: A total of 255 AFB smear-positive respiratory specimens were studied. For the differentiation of MTB from NTM, the bands of the 360 bp, which exists both in MTB and NTM, and the 190 bp, which exists only in MTB, were amplified using the onetube nested PCR targeting regions of the rpoB gene. The two-tube nested PCR targeting 16S-23S rRNA spacer gene was done with 34 specimens that were negative by one-tube nested PCR. The specimens that were tested positive for NTMs were subsequently subjected to PCR-restriction fragment length polymorphism (PCR-RFLP) analysis based on the rpoB gene for mycobacterial species identification. RESULTS: Detection rate of MTB and NTM was 87% after the one-tube nested PCR. The detection range of MTB and NTM increased up to 93% after the two-tube nested PCR. The results of PCR-RFLP analysis identified those NTMs as M. avium and M. intracellulare. CONCLUSION: This result seems to suggest strongly that the PCR testing especially aiming for differentiation of MTB from NTM, and identificatin of mycobacterial species using AFB smear-positive specimens would be highly importnat in clinical settings for effective treatment of patients.
Humans ; Mycobacterium tuberculosis* ; Mycobacterium* ; Nontuberculous Mycobacteria* ; Polymerase Chain Reaction*