Purpose To study the clincal and pathological characterization of autoimnune hapatitis(AIH)cases. Methods The clinical data, the results of pathological examination, immtmofluorescence stainingobservation of the liver biopsy tissue of 8 AIH cases were investigated retrospectively. Results All of 8patients were female and were diagnosed as type Ⅰ AIH. The main symptoms included fever, arthrodynia,purpura or jaundice. Most of cases were companied with other autoimmune diseases and were positive for RFor/and ANA antibodies. The titer of ganna globulin, ALT and AST was increased. The chronic hepatitiswas the main histologic change, in which the minor to moderate degree was in most cases with spotty,piecemeal and bridging necrosis of liver tissue, inflammatory infiltration and fibrosis processing in portal area.In 5 cases IgG was detected by immunofluorescence assay,and in 2cases HBsAg was positive. ConclusionsThe histologic and immunofluorescence examination are necessary for the diagnosis of AIH and can behelpful especially in the diagnosis of the variant type of AIH.

Oxidative stress and inflammation are two main factors in the progress of diabetic nephropathy(DN) and its complications. NF‐E2 p45‐related factor (Nrf2) is a crucial transcriptional factor which manipulates downstream genes that encode some antioxidant enzymes and phase Ⅱ detoxifying enzymes ,to maintain the redox homeostasis and cellular detoxification response. Therefore ,more and more researchers are focusing on the role of Nrf2 in DN. In this review ,the detailed role of Nrf2 in DN will be discussed. Hopefully ,our work can epitomize recent research progress and provide novel clues for diabetic nephropathy prevention and treatment.

Objective To investigate the sleep problems and related affecting factors of primary or possibly symptomatic school-aged children with epilepsy.Methods 54 epilepsy children(7 to 12 years old) and the general 54 healthy children whose gender and age were strictly matched with epilepsy group were investigated by using the chinese version of CSHQ.Results ① The total CSHQ score and 6 subscale scores (bedtime resistance, sleep onset delay,sleep anxiety, night wakings, parasomnias, and daytime sleepiness)were significantly higher in the epilepsy group(P<0.05).② In the epilepsy group, different age groups had statistical difference in the total CSHQ score and 4 subscale scores(sleep resistance, sleep onset delay, sleep duration and sleep anxiety) (P<0.05).The seizure controlled group and the uncontrolled group had statistical difference in the total CSHQ score and all subscale scores, except sleep onset delay(P<0.05).There were statistically significant differences in the total CSHQ score and all subscale scores, except sleep-disordered breathing in different seizure frequency groups(P<0.05).Different seizure type groups had statistical difference in the total CSHQ score and 4 subscale scores(sleep resistance, sleep duration, sleep anxiety and daytime sleepiness)(P<0.05).Different seizure time groups had statistical difference in the total CSHQ score and 5 subscale scores(sleep resistance, sleep onset delay, sleep duration, sleep anxiety and night waking)(P<0.05).Monotherapy group and polytherapy group had statistical difference in the total CSHQ score and 4 subscale scores(sleep onset delay, night waking, parasomnias and daytime sleepiness) (P<0.05).③ Multiple linear regression analysis showed that the main influencing factors of the total CSHQ score and some subscale scores were gender, age, control of seizure, frequency of seizure, type of seizure, time of seizure and the number of drug taking.Conclusion Children with epilepsy compared with healthy children are more likely to have sleep problems, and the occurrence of sleep problems may be related to many factors.

Objective To further understand the effect of anti-fluoride water on intellectual development of children and dental fluorosis in drinking water-type of endemic fluorosis area Pucheng county Shaanxi province.Methods Two hundred 8 to 12 years old children were randomly selected in endemic fluorosis area with changed water or unchanged water, in 2009 in Pucheng county Shaanxi province. Intelligence quotient(IQ) of children was measured by Raven's test. Children's dental fluorosis was examined by Dean's classification scheme. Results The rates of child dental fluorosis in changed water and unchanged water endemic fluorosis areas were 28.50%(57/200), 87.88%(203/231) , respectively, the difference was statistically significant(x2 = 159.19, P＜ 0.01),while dental fluorosis indices were 0.57,1.97, was marginal, moderate epidemic. Children with IQ above 90 was accounted for 89.5% (179/200), 91.5% (183/200) in changed water and unchanged water endemic fluorosis area,respectively, the difference was not significant (x2 = 0.46, P ＞ 0.05). Conclusions Changed water may decrease the incidence of child dental fluorosis, but has no obvious effect on children's IQ development in endemic fluorosis area.

Objective To investigate the effects of ephedrine on glial fibrillary acidic protein (GFAP) expression after cerebral ischemia-reperfusion injury in rats.Methods Altogether 60 male SD rats were randomly divided into sham-operation group,natural recovery group and ephedrine treatment group.The unilateral ischemia-reperfusion models were induced by clue-blocked method.The expression level of GFAP around ischemic area was examined by immunohistochemical technique at weeks 1,2,3 and 4 after operation.Results GFAP expression began to increase at 1 w and stabilized at 3 w in ephedrine treatment group and natural recovery group.There was a significant increase of GFAP expression in ephedrine treatment group compared with that in natural recovery group (P

Objective To explore the effects of ephedrine on the hippocampal cell apoptosis and behavioral performance after hypoxia-ischemia brain injury in neonatal rats.Methods Totally 90 7-day rats were randomly divided into 3 groups,ephedrine treatment group,model group,and sham group.Hypoxia-ischemia brain injury model was established by permanently ligating right common carotid artery.Ephedrine (1.5 mg/kg,once per day) was injected intraperitoneally to the rats of ephedrine treatment group for 7 d,and the rats of model group was given normal saline at the same volume.At the following time interval of 6 and 12 h,and 1,3,and 7 d after hypoxia,the expression of bcl-2 and bax were detected in the hippocampal region by immunohistochemical staining.At 4 weeks after surgery,behavioral changes in the remaining rats were tested by Morris water maze.Results Compared with model group,the expression of bcl-2 in the ephedrine treatment group was significantly increased after hypoxic-ischemic injury,peaked at 1 d and decreased in 3 d after operation.And the expression of bax in the ephedrine treatment group was decreased in 1 d after hypoxic-ischemia.The average time of escape latency was gradually decreased in each group.However,from the 3rd to 5th day,it was much shorter in ephedrine treatment group than in model group.In addition,the frequency platform passing in the ephedrine treatment group and the percentage of swimming distance traveled in the previous target quadrant was significantly greater than those of the control group.Conclusion Ephedrine upregulates bcl-2 and downregulates bax in the hippocampus of neonatal rats after hypoxia-ischemia,and improves their ability of learning and memory.

Objective To observe the protection of D-amphetamine on rat brain after focal cerebral ischemia.Methods The unilateral middle cerebral artery occlusion(MCAO) models were established by using Koizumi's method.TUNEL was applied to detect quantitatively brain cell apoptosis at 1st,3rd and 6th week after operation.Immunohistochemical staining and RT-PCR were respectively used to detect the expression of growth-associated protein 43(GAP-43) and GAP-43 mRNA around ischemic area.Results Apoptosis of brain cells reduced evidently in the group treated with D-amphetamine.GAP-43 protein detection demonstrated statistically significant increase in immunoreaction product as determined by optical density measurements in D-amphetamine treated group compared with the group without any agent treatment.The same results appeared in RT-PCR product.Conclusion D-amphetamine can reduce brain cell apoptosis and promote GAP-43 expression.

Objective:To screen fine germplasm resources and suitable cultivated place for Paris polypyh lla var.yunnanensis by determining the content of total saponins , total flavonoids and total polysaccharides collected from Yunnan and Guizhou .Methods:An Ultraviolet-visible spectrophotometry method was applied to determine the total contents of saponin , flavonoid and polysaccharide in 14 germplasm resources of Paris polyhp ylla var.yunnanensis.Results:The contents of saponins, flavonoids and polysaccharides in Paris polyphylla var.yunnanensis collected from the different habitats were obviously different .The herb with high quality was selected from Xiaguan and Dali with the highest total content of saponins and the content of the three components was 55.760 0 and 61.632 0 mg· g-1 , respectively , which was significantly higher than that from the other habitats .Conclusion: It is proved that the quality of Pa ris polyphyll a var.yunnanensis produced in Dali of Yunnan is better than that from the other regions , and the high yield culture techniques should be studied further .

Objective To explore the role of connective tissue growth factor(CTGF)in the mechanism of liver fibrosis.Methods Liver fibrosis models of rats were made by subcutaneously injecting with CCl4.On the other hand,32 human liver cirrhosis and 12 normal liver tissues were collected too.Immunohistochemical staining was used to detect the expression of CTGF in these tissues.Results The expression of CTGF in rats after injection with CCl4 was found mainly in hepatic stellate cells(HSC) and hepatocytes are all significantly higher than those in normal rats.Moreover,it presents with progressive tendency for the expression of CTGF in rats respectively at 1st,4th and 8th week after injection of CCl4.The same tendency was found in human normal and fibrotic liver tissues(P