Combining the experiment teaching innovation in our college,we carried on beneficial quest to the teaching mode of the biochemistry experiment.During the practice of the designing experiment,we not only pay attention to cultivating students' creation ability,but also lay emphasis on cultivation of their autonomy ability.which is very important to improve the students,early scientific research abilities or clinical practice abilities and so on.

Asthma was considered to be a kind of chronic airway inflammatory diseases mediated by eosinophils(EOS),mast cells,T lymphocytes for a long time,and the typical pathologic features of asthma was airway EOS inflammation.The current study had found out that elevated neutrophils in airway were seen in severe asthma,and this kind of asthma had a poor response to corticosteroids.Impaired neutrophil chemotaxis and apoptosis of airway neutrophilia may be associated with persistent neutrophilic inflammation in the airways of severe asthma.A deep research into the mechanisms of neutrophilic phenotypes asthma would contribute to the new strategy of therapy.This article discusses a range of topics related to the role of neutrophilic inflammation in severe asthma in children,which were organizedas follows.

Obesity has been a world-wide growing health problem in children and adolescents,insulin resistance plays a pivotal role in the pathogenesis of obesity-associated complications,the mechanism is still unknown.There is growing evidence that obesity is associated with low-grade inflammation in youth.The onset of low-grade inflammation in obesity may be associated with the inflammatory cytokine secreted by adipose tissue,local Monocyte/Macrophage system and role of oxidative stress.The study of inflammatory cytokines in pathogenesis of obesity and insulin resistance will help to develop new treatment strategies to prevent obesity and obesity-associated complications.

The accurate medical treatment is based on the information of the genome, which is the best treatment for the patients. Population pharmacokinetic study can be formulated according to the individual differences of patients to the dose, in the accurate medical model which has a unique advantage. At present, there are many problems such as adverse drug reaction in Chinese traditional medicine, and it is necessary to introduce a group of medicine on the basis of precise medical treatment. However, due to the different characteristics of traditional Chinese medicine and chemical medicine, it is necessary to combine the population pharmacokinetics, genetics and statistical methods to establish a research method which is in line with the characteristics of Chinese medicine. The key scientific problem is to make clear the active components of Chinese medicine metabolism of the drug metabolic enzyme gene, and pay attention to the analysis of the polymorphism of the overall role of drug metabolism enzymes in the human body. Clear key scientific issues and break through the bottleneck, so as to achieve the precise medical treatment, to international.
Humans ; Medicine, Chinese Traditional ; Pharmacokinetics

0.05) ,but the differences reached statistical significance compared the positive ratios of two index together to urine NAG and ?2 - MG (X2 = 4.41,7.28 P

Objective To study the adjuvant effects of chitosan on Helicobacter pylori (H.pylori) vaccine.Methods A total of 20 adult mice were randomly divided into four groups.Mice in groups Ⅰ,Ⅱ,Ⅲ, and Ⅳ were orally immunized with PBS,H.pylori antigen alone,H.pylori antigen plus chitosan solution or H. pylori antigen plus chitosan particles,respectively.An ELISA was used to detect anti-H.pylori IgA in saliva and gastric mucosa ,interlukin(IL)-2,IL-4,IL-10 levels in gastric mucosa.Immunohistochemical method was used to detect secretory IgA in gastric mucosa.Results ①The levels of special anti-H.pylori IgA in saliva and gastric mucosa in the groups with chitosan as adjuvant were significantly higher than those in the group without adjuvants and control groups(P

After percutaneous coronary intervention (PCI) was performed,since CHD risk factors still exist, coronary restenosis rate remains high.Therefore, self-management after PCI is very important.The present article made a review on current condition and research progress of self-management in patients after PCI, aiming at providing reliable evidence for rehabilitation after PCI.

Objective To investigate the correlations between plasma levels of carnitine(CT),free fat acid(FFA) and insulin resisitance in children with simple obesity.Methods Fifty-six children diagnosed with simple obesity were enrolled as study group(obesity group),36 healthy children as control group.The concentration of plasma level of insulin was measured by radioimmunoassay(RIA),CT was measured by high performance liquid chromatography(HPLC),FFA and triglyceride(TG) were measured by enzymatic-colorimetric assay.Body mass index(BMI) and waist to hip ratio(WHR) were caculated.Insulin sensitivity index (InSI) and insulin resistance index (InRI) were cacula-ted by homeostasis model assessment of insulin resistance (HOMA-IR).SPSS 13.0 software was used to analyze the data.Results The concentration of CT in plasma was (43.67?12.75) ?mol/L in obesity group,(58.31?21.25) ?mol/L in control group,respectively.There was a significant difference between 2 groups (t=2.566 P

Objective To study the expression of signal transducers and activators of transcription 3(STAT3) in airway epithelial cells of asthmatic mouse model and its association with airway remodeling,explore the role of signal-transduction pathway in airway remodeling.Methods Thirty Balb/c mice were randomly divided into normal control group(n=10),asthma without intervention group(n=10) and AG490 intervention group(n=10).The mice were sensitized with ovalbumin to establish the asthmatic model.The histological changes were evaluated by HE staining,total brochial wall thickness(Wat)and smooth muscle thickness(Wam) were measured by image analysis system,the percentages of collagen deposition were detected by Masson′s trichrome staining,the expression of STAT3 in airway were detected by immunohistochemistry technique;lung tissue extracts were analyzed for phosphorylation of STAT3(p-STAT3)by Western blot.SPSS 13.0 software was used to analyze the data.Results 1.The histological changes including airway thickness,airway smooth cell proliferation and excessive collagen deposition in subepithelial aera were found under light microscope in asthmatic mice.2.The level of STAT3 and p-STAT3 in asthma without intervention group were significantly higher than those in normal control group(Pa