Introduction: The junction between midpalatal suture is one of the easiest patterns for investigating the relationship between bone formation and resorption. Suture widening for the therapy of transverse maxillary deficiency, by elucidating molecular pathways from previous studies, which provide basic knowledge on bone homeostasis. The research purpose to analysed the regulation of osteogenesis and osteoclastogenesis through the ALP, TRAF-6, and midpalatal area after maxillary suture expansion, induced by the Hyperbaric Oxygen Therapy (HBOT) 2,4 ATA administration from day 8-14. Methods: The sample used 18 male Cavia cobaya aged 2-3 months, which were divided into three groups: Normal control K(O), negative control with expansion appliance K(-), and HBOT (P). After 14 days, Cavia cobaya were decapitated, followed by the horizontal cutting of the maxilla, and preparation into slides. Osteoclast and osteoblast number, as well as the ALP and TRAF-6 expressions, and the midpalatal areas were counted. Results: Statistical analysis using Kruskall Wallis showed significance in all groups (p≤0.05), while Pearson correlation test show the highest correlation between osteoblast and ALP (r=0.951), osteoblast and osteoclast (r=0.848), ALP and osteoclast (pr=0.745), as well as ALP and midpalatal (r=0.704). Conclusion: The treatment group demonstrated elevated levels of osteoclast and ALP expression, which collectively regulate the bone remodeling process, as well as osteoblasts, and the midpalatal area, with a decline in TRAF-6. Osteoblast has a role during the interaction of endothelial cells and osteoclasts in osteogenesis, while ALP is involved in the mineralization process of the bone matrix within the midpalatal area.