1.Endometritis associated with Enterococcus casseliflavus in a mare:A case report
Nocera Paola Francesca ; Papulino Chiara ; Prete Del Chiara ; Palumbo Veronica ; Pasolini Pia Maria ; Martino De Luisa
Asian Pacific Journal of Tropical Biomedicine 2017;7(8):760-762
Infectious endometritis is one of the main causes of subfertility/infertility in the mare. In this report, we present the first case of endometritis in mare associated with a strain of Enterococcus casseliflavus, an unusual gram-positive bacterium which can also be a zoonotic agent. Furthermore, the isolated strain showed a worrying multidrug-resistant profile. The accurate finding of a successful antimicrobial treatment and consequently, the pregnancy diagnosis indicate the importance to isolate, identify and define the anti-biotic resistance profile of bacteria associated with endometritis.
2.An update on microbiological causes of canine otitis externa in Campania Region, Italy
Martino De Luisa ; Nocera Paola Francesca ; Mallardo Karina ; Nizza Sandra ; Masturzo Eleonora ; Fiorito Filomena ; Iovane Giuseppe ; Catalanotti Piergiorgio
Asian Pacific Journal of Tropical Biomedicine 2016;6(5):384-389
Objective: To update the recent knowledge of the microbiological causes of canine otitis externa in Campania Region (Italy) and the antibiotic susceptibility patterns of the iso-lated strains.
Methods: A total of 122 dogs were examined by otoscopy, and auricular swab samples were collected from both ears in 74 dogs presenting clinical bilateral otitis and from single ears in 48 dogs displaying clinical unilateral otitis. Cytological examination, bacteriological analysis and antimicrobial susceptibility tests were performed.
Results: Thirty-one out of 122 dogs were positive for yeast species (25.4%, 95% con-fidence interval (CI): 18.2%–34.2%) with a higher prevalence of Malassezia pachy-dermatis (21/31 isolates, 67.7%, CI: 48.5%–82.7%), and a total of 91 out of 122 dogs were positive for bacterial species (74.6%;CI:65.8%–81.8%) with a higher prevalence of Staphylococcus pseudintermedius (45/143 isolates, 31.5%, CI: 24.1%–39.8%). These results are the first description of Streptococcus agalactiae-associated otitis. The yeasts isolated showed high levels of susceptibility to all antifungal agents tested; on the con-trary all the isolated bacterial strains were highly resistant to at least four out of ten antimicrobial classes. Both Gram-positive and Gram-negative bacteria showed high resistance to amoxicillin/clavulanate and kanamycin hence they are not recommended as initial empirical therapy for the otitis treatment.
Conclusions: This update illustrates an increase in antibiotic resistances providing an insight into the current knowledge of the therapeutic procedures followed on canine otitis externa in Italy. It also emphasizes the importance of considering the results of the microbiological and sensitivity tests to decide on an appropriate antibiotic therapy.
3. Endometritis associated with Enterococcus casseliflavus in a mare: A case report
Francesca Paola NOCERA ; Chiara PAPULINO ; Chiara DEL PRETE ; Veronica PALUMBO ; Maria Pia PASOLINI ; Luisa DE MARTINO
Asian Pacific Journal of Tropical Biomedicine 2017;7(8):760-762
Infectious endometritis is one of the main causes of subfertility/infertility in the mare. In this report, we present the first case of endometritis in mare associated with a strain of Enterococcus casseliflavus, an unusual gram-positive bacterium which can also be a zoonotic agent. Furthermore, the isolated strain showed a worrying multidrug-resistant profile. The accurate finding of a successful antimicrobial treatment and consequently, the pregnancy diagnosis indicate the importance to isolate, identify and define the antibiotic resistance profile of bacteria associated with endometritis.
4. An update on microbiological causes of canine otitis externa in Campania Region, Italy
Luisa DE MARTINO ; Francesca Paola NOCERA ; Karina MALLARDO ; Sandra NIZZA ; Eleonora MASTURZO ; Filomena FIORITO ; Giuseppe IOVANE ; Piergiorgio CATALANOTTI
Asian Pacific Journal of Tropical Biomedicine 2016;6(5):384-389
Objective: To update the recent knowledge of the microbiological causes of canine otitis externa in Campania Region (Italy) and the antibiotic susceptibility patterns of the isolated strains. Methods: A total of 122 dogs were examined by otoscopy, and auricular swab samples were collected from both ears in 74 dogs presenting clinical bilateral otitis and from single ears in 48 dogs displaying clinical unilateral otitis. Cytological examination, bacteriological analysis and antimicrobial susceptibility tests were performed. Results: Thirty-one out of 122 dogs were positive for yeast species (25.4%, 95% confidence interval (CI): 18.2%-34.2%) with a higher prevalence of Malassezia pachydermatis (21/31 isolates, 67.7%, CI: 48.5%-82.7%), and a total of 91 out of 122 dogs were positive for bacterial species (74.6%; CI: 65.8%-81.8%) with a higher prevalence of Staphylococcus pseudintermedius (45/143 isolates, 31.5%, CI: 24.1%-39.8%). These results are the first description of Streptococcus agalactiae-associated otitis. The yeasts isolated showed high levels of susceptibility to all antifungal agents tested; on the contrary all the isolated bacterial strains were highly resistant to at least four out of ten antimicrobial classes. Both Gram-positive and Gram-negative bacteria showed high resistance to amoxicillin/clavulanate and kanamycin hence they are not recommended as initial empirical therapy for the otitis treatment. Conclusions: This update illustrates an increase in antibiotic resistances providing an insight into the current knowledge of the therapeutic procedures followed on canine otitis externa in Italy. It also emphasizes the importance of considering the results of the microbiological and sensitivity tests to decide on an appropriate antibiotic therapy.