Over the past few years, considerable progress has been achieved about the extracellular elements and intracellular regulatory molecules that are involved in the regulation of chondrogenesis. However, little is known about the molecular mechanism of how these molecules influence the gene activities during cartilage differentiation. Recently we isolated a Chicken Cartilage derived Matrix Protein (CCMP 10), a novel protein, from chicken prechondrogenic mesenchyme. To further understand the function of CCMP-10 in cartilage development, we investigated the expression of CCMP-10 during the prechondrocyte differentiation in chick embryos and micromass cultured prechondrogenic cells, using a variety of methods such as transient transfection of CCMP 10, immunohistochemical localization, northern analysis, and western analysis. When transiently transfected, CCMP 10 was expressed in both nucleus and cytoplasm, with stronger intensity in the nucleus. In an immunohistochemical study, CCMP 10 was expressed in prechondrogeinc mesenchymal cell, perichondrium, and resting and proliferative zone of the growth plate of long bone, while no expression of CCMP 10 was observed in upper mature chondrocytes and hypertrophic chondrocytes. Northern analysis of micromass cultured prechondrogenic cells showed the expression of CCMP-10 mRNA for first 2 days, while Col 2a1, aggrecan, and CMP mRNAs, known genes to express in mature chondrocyte, initiated the expression at day 2 and continued to express by day 5. In western analysis, CCMP-10 was detected at initial stage and continued to express by day 3, while Col 2al protein began to express only one day after, and continued to express. Taken together, our data suggest that CCMP-10 may play a significant role in the early cartilage development.
Aggrecans ; Animals ; Cartilage* ; Chick Embryo ; Chickens* ; Chondrocytes ; Chondrogenesis* ; Cytoplasm ; Growth Plate ; Mesoderm ; RNA, Messenger ; Transfection

The World Health Organization (WHO) have been in collaborative efforts with the Republic of Korea in keeping of and for better health for all for the past decades. From the control of parasites to building of community health system in rural places, the works has now resulted in healthier Korea than ever, and has transformed the role of engaging as the world leader in contribution of health and development. Seventy years of independence, war, and poverty, transforming from a recipient country of official development assistance to a significant donor to the global society, we have emphasized the importance of international cooperation and the role of WHO in the past years in Korea and neighboring countries. Looking back of the past is meaningful to diagnose the present problems, and to foresee the future of our world.
Community Health Planning ; Global Health* ; Humans ; International Cooperation ; Korea ; Parasites ; Poverty ; Public Health ; Publications* ; Republic of Korea* ; Tissue Donors ; World Health Organization*

BACKGROUND: While smoking prevalence in Korean men has been decreasing, it is increasing in Korean women. Little is known about women's smoking inequalities in Korea. This study was conducted to investigate the association of socioeconomic indicators with the initiation and cessation of smoking among Korean women. METHODS: This was a cross-sectional study on 9,089 women aged 25-64 years from the 2008 Seoul Community Health Survey. The data on smoking and socioeconomic status were obtained through face-to-face interviews. Smoking initiation rate was defined as the proportion of the individuals who had started smoking at least one cigarette among all subjects. Smoking cessation rate was calculated by dividing the number of individuals who had quit smoking by the number of ever smokers. Education level, total family income and occupation were investigated as socioeconomic indicators. RESULTS: Education level was significantly associated with both initiation and cessation of smoking. Lower educated women had a higher likelihood of smoking initiation (odds ratio [OR], 1.72; 95% confidence interval [CI], 1.17 to 2.51) but lower likelihood of smoking cessation (OR, 0.38; 95% CI, 0.22 to 0.66) than higher educated women. Smoking initiation rate was higher in manual workers (OR, 1.65; 95% CI, 1.20 to 2.27) than in non-manual workers. However, there were no significant differences of both initiation and cessation of smoking according to total household income. CONCLUSION: This study shows that there are smoking inequalities among Korean women. It is thought that education level and occupation are important determinants of women's smoking status.
Aged ; Cross-Sectional Studies ; Family Characteristics ; Female ; Health Surveys ; Humans ; Korea ; Male ; Occupations ; Prevalence ; Smoke ; Smoking ; Smoking Cessation ; Social Class ; Socioeconomic Factors ; Tobacco Products

OBJECTIVES: We estimated the population prevalence of antibodies to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), including unreported infections, through a Korea Seroprevalence Study of Monitoring of SARS-CoV-2 Antibody Retention and Transmission (K-SEROSMART) in 258 communities throughout Korea. METHODS: In August 2022, a survey was conducted among 10,000 household members aged 5 years and older, in households selected through two stage probability random sampling. During face-to-face household interviews, participants self-reported their health status, COVID-19 diagnosis and vaccination history, and general characteristics. Subsequently, participants visited a community health center or medical clinic for blood sampling. Blood samples were analyzed for the presence of antibodies to spike proteins (anti-S) and antibodies to nucleocapsid proteins (anti-N) SARS-CoV-2 proteins using an electrochemiluminescence immunoassay. To estimate the population prevalence, the PROC SURVEYMEANS statistical procedure was employed, with weighting to reflect demographic data from July 2022. RESULTS: In total, 9,945 individuals from 5,041 households were surveyed across 258 communities, representing all basic local governments in Korea. The overall population-adjusted prevalence rates of anti-S and anti-N were 97.6% and 57.1%, respectively. Since the Korea Disease Control and Prevention Agency has reported a cumulative incidence of confirmed cases of 37.8% through July 31, 2022, the proportion of unreported infections among all COVID-19 infection was suggested to be 33.9%. CONCLUSIONS The K-SEROSMART represents the first nationwide, community-based seroepidemiologic survey of COVID-19, confirming that most individuals possess antibodies to SARS-CoV-2 and that a significant number of unreported cases existed. Furthermore, this study lays the foundation for a surveillance system to continuously monitor transmission at the community level and the response to COVID-19.