It has been reported that the Ca2+-ATPase and the Ca2+-Na+exchanger play an important role for the regulation of intracellular Ca2+ in somatic cells, the Ca2+-ATPase located in the plasma membrane helps the Ca2+ concentration in maintain low [Ca2+]i. Roldan & Fleming reported that the spermatozoan Ca2+-ATPase plays an important role in the capacitation and acrosome reaction. We used to assess Ca2+ changes by chlortetracycline (CTC) patterns in the capacitation and acrosome reaction of human and hamster spermatozoa. In the present study applying quercetin which has been known as an ATPase antagonist, the enzymatic effect of Ca2+-ATPase on capacitation and acrosome reaction was found to be remarkable: a significant increase of the transformation from the original type to the B type and the AR type of spermatozoa. This finding suggests that Ca2+-ATPase play an important role in the efflux and the influx of the Ca2+ which have been known to be an essential factor for the capacitation and acrosome reaction, and that the inhibitory action of the Ca2+-ATPase might be a prerequsit step toward the capacitation and acrosome reaction. In conclusion, this study suggest the considerable evidence as follows: the increment of the intracellular Ca2+ concentration occurred by controlling the slope of Ca2+ concentration through Ca2+-ATPase activites in both the intracellular and extracellulr fluid may be important procedures for the capacitation and the acrosome reaction, and finally for fertilization of the sperm and ovum.
Acrosome Reaction* ; Acrosome* ; Adenosine Triphosphatases ; Animals ; Cell Membrane ; Chlortetracycline* ; Cricetinae ; Fertilization ; Fluorescence* ; Humans ; Male ; Ovum ; Quercetin ; Spermatozoa

A clinical observations of skin lesions was performed on 189 patients suffering from hepatitis who were admitted to the Department of Internal Medicine in Armed. Forces General Hospital. The result were as follows: 1) Cutaneous manifestations were found in 143 patients(75.7%). 2) Purpuric lesion was found in 62 patients(32. 8%), spider angioma in 57 patients(30%), palmar erythema in 40 patients(21.2%), melanosis in 37 patients(19. 6%) acne in 22 patients(11,6%), jaundice in 15 patients(7.9%) and striae distensae in 14 patients(7.4%). 3) Among rnelanosis, there were 14 patients with diffuse pigmentation, 3 patients with localized pigmentation, 14 patients with palmar crease pigmentation, 10 patients with accentuation of freckling and 11 patients with chloasma. 4)Common cutaneous manifestations in acute hepatitis were spider angioma (21. 4%), palmar erythema (21.4%) and purpuric lesion (17.9%), in chronic hepatitis, purpuric lesion(44.8%) spider angioma(37%) and melanosis(24.8%).
Acne Vulgaris ; Arm ; Erythema ; Hemangioma ; Hepatitis* ; Hepatitis, Chronic ; Hospitals, General ; Humans ; Internal Medicine ; Jaundice ; Melanosis ; Pigmentation ; Skin ; Spiders ; Striae Distensae

A clinical observation of self-inflicted lesions was made during physical examination for conscription in Choongcheongnamdo from May to July, 1980. The result were as follows: 1) The prevalence of self-inflicted lesions was 8. 1% (579/7148). 2) The number of persons with criminal history was 68(ll.7%). 3) The educational level of self-injured was lower than that of non self-injured. 4) Most frequently used tools for self-inflicted lesions were cigarette(72%), knife(19. 9%), rnatch(14.7%) and grass(12.8%) in decreasing order of frequency and only one tool was used in 438 cases(75.6%). 5) The favorite site of self injury were the left forearm(89. 5%), right forearm (19.3%) and left upper arm (5.9%) 6) The average age of first injury was 16. 5 years. 7) The complications of self injury were found in 8. 8% of self-injured and they were hypertrophic scar, keloid, pyoderma and depigmentation. Some degree of hyperpigrnentation and hypopigmentation was found in most of cases. Tattoo was accompanied in 20. 7% of self-injured.
Adolescent* ; Arm ; Cicatrix, Hypertrophic ; Criminals ; Forearm ; Humans ; Hypopigmentation ; Keloid ; Physical Examination ; Prevalence ; Pyoderma

No abstract available.
Embryonic Structures* ; Humans*

BACKGROUND: Recently the sensitive measurement of radiation damage to DNA using flowcytometric analysis of nucleoid preparations was reported which allows an analysis of damage within the DNA of single cells. We applied flowcytometric analysis of the nucleoids for the detection of DNA damage by UVB. OBJECTIVE: The purpose of this study was to establish the method of detecting UVB-induced damage of DNA by flowcytometry and to elucidate the usefulness of this method to detect cell damage. METHODS: Human melanoma cells were cultured and were irradiated with various of UVB. Immediately after UVB irradiation nucleoid suspensions were prepared and flowcytometric analysis was done. RESULTS: The changes in fluorescence, forward scatter, and side scatter reflected damage of DNA induced by UVB quite well especially at lower UVB doses. CONCLUSION: Flowcytometric analysis of nucleoid will be a useful methosd to detect DNA damage by UVB.
DNA ; DNA Damage ; Fluorescence ; Humans ; Melanoma ; Methods ; Suspensions ; Ultraviolet Rays*

Septic arthritis in neonates is a rare condition. A failure to make an early diagnosis of septic arthritis in neonates may leave a permanent disability as a result of a delayed treatment. Thus, septic arthritis, requires a prompt diagnosis and a timely treatment especially in this subset of patients. In this case report, we describe our treatment protocol for septic arthritis and concurrent osteomyelitis in the right shoulder of a 28-day-old newborn. Using 2.4 mm wrist arthroscopy, we performed an arthroscopic irrigation and drainage, to remove intra-articular debris and inflammatory tissue, and multiple drilling. We report a satisfactory clinical outcome without any postoperative complications or side effects.
Arthritis, Infectious* ; Arthroscopy ; Clinical Protocols ; Diagnosis ; Drainage ; Early Diagnosis ; Humans ; Infant, Newborn* ; Osteomyelitis ; Postoperative Complications ; Shoulder Joint ; Shoulder* ; Wrist

BACKGROUND: Clinical features of atopic dermatitis(AD) have been well described according to age in the English literature. In Korea there have been some reports about clinical analyses of AD. However, there is no report about the clinical characteristics of adult AD. OBJECTIVE: We tried to characterize the clinical features of adult AD by comparing them with the childhood/infantile type. In addition we aimed to study the prognostic factors that may be related to the persistence of dermatitis into adult life. METHODS: Patients with AD were divided into the adult and childhood/infantile types according to the patient's age. Sixty three adult patients were compared with 107 childhood/infantile patients. Various clinical characteristics of AD were compared between these groups. RESULTS: Orbital darkening, foot eczema, and extensor involvement were more frequently observed in the childhood/infantile type, whereas flexural lichenification, especially in the antecubital fossa, prurigo papules in the extensor part of the extremities, cheilitis, forehead lichenification, Hertoghe's sign, nipple eczema, and anterior neck fold involvement were more frequent in the adult type. Light sensitivity was more prominent in the adult type. Later age at onset of AD was observed in adult patients. The incidence of respiratory atopy (asthma and allergic rhinitis) was higher in the adult group. CONCLUSION: In the adult type flexural involvement with lichenification became more prominent and prurigo papules were more frequently observed in the extensor part of the extremities. Later onset of AD and association with respiratory atopy were considered to be bad prognostic factors.
Adult* ; Age of Onset ; Cheilitis ; Dermatitis ; Dermatitis, Atopic* ; Eczema ; Extremities ; Foot ; Forehead ; Humans ; Incidence ; Korea ; Neck ; Nipples ; Orbit ; Photophobia ; Prurigo

Primary cystic neoplasia of the liver is rare. We report a cystadenoma with mesenchymal stroma (CMS) and a cystadenocarcinoma, and make a review of literature with particular reference to their histogenesis. The CMS has many similarities to the ovarian mucinous cystadenoma; occurring almost exlusively in female, being lined by mucus-secreting epithelial cells, and containing dense ovarian-like stroma. These features suggest that CMS may arise from the ectopic ovarian tissue within the liver. Cystadenocarcinoma may have its origin in CMS or cystadenoma without mesenchymal stroma of CMS. Cholangiocarcinoma arising from the congenital hepatic cysts can be differentiated only when it contains benign epithelia.
Female ; Humans ; Cysts

No abstract available.
Animals ; Rats*

No abstract available.
Blood Platelets* ; Humans ; Infant, Newborn ; Infant, Premature* ; Phototherapy* ; Platelet Count*