Objective: To investigate ApoD expression in preoperative serum and tissues of patients with esophageal squamous cell car-cinoma of different survival terms, to explore its correlation with disease prognosis, and to speculate whether it can act as a biomarker for predicting the prognosis of patients with esophageal cancer. Methods: A total of 731 patients with radical resection of esophageal cancer were collected from Anyang Tumor Hospital in an area of high incidence of esophageal cancer in Henan Province from March 2008 to September 2009. General data, serum samples, and tissue specimens were collected. Patients with two extreme survival terms (34 cases)-a survival period≤3 years and a survival period≥5 years were randomly selected from the database, and the healthy population was used as the normal control group. iTRAQ combined with MALDI-TOF/TOF MS was used to analyze the preoperative se-rum proteins in patients with esophageal cancer and lock the target protein ApoD. Western blot analysis was used to verify the level of ApoD expression in preoperative serum and healthy individuals with esophageal squamous cell carcinoma of different survival terms. Immunohistochemical techniques were used to detect the expression level of ApoD in esophageal cancer tissues and normal tissues. Results: The results of iTRAQ combined with MALDI-TOF/TOF MS showed that the expression of 52 proteins was up-regulated with prolonged survival, and the difference was significant; ApoD was one amongst them. Western blot results showed that ApoD had the highest serum expression in the≥5 years survival group, followed by normal human serum, and the lowest expression in the group having survival period≤3 years. The difference was statistically significant. Immunohistochemistry results showed that ApoD exhibited the highest expression in normal esophageal squamous epithelium, followed by the ≥5 years survival group, and lowest in the ≤3 years survival group. The difference was statistically significant. Conclusions: The expression of ApoD is positively correlated with the survival of patients with esophageal cancer, which may be related to the prognosis of patients with esophageal cancer. Thus, ApoD could be a potential biomarker for predicting the prognosis of patients with esophageal cancer.

Objective: To assess the food and nutrition literacy level of school-age children and its related factors, and to provide targets for further nutrition health education and nutrition improvement action. Methods: A total of 4 359 school-age children, from third to eighth grade in 2 middle schools and 3 primary schools of Baoding city of Hebei Province, were selected as subjects using convenient sampling method. For all participants’ food and nutrition literacy evaluation, social demographic characteristics and family food environment were investigated. Results: The average score of food and nutrition literacy of valid respondents was (61.91±9.22), and the scores of functional, interactive and critical literacy increased successively. The score of food and nutrition knowledge was higher than that of skill, and the score of "food intake" skill was the lowest(60.45±11.00). Children’s food and nutrition literacy increased with age and grade during primary school period, but the literacy of junior school students was lower than that of high-level graders in primary school(P<0.05). Children who were female, the only children, non-resident in school, urban registered permanent residence, in a more affluent family, cared by their parents/grandparents with a higher education level, and had nutrition education experience in school, had significantly higher food and nutrition literacy(P<0.05). But the interactive literacy of only children, non-resident in school and urban registered children was significantly lower(P<0.05). The family food environment was significantly correlated with children’s food and nutrition literacy. The total score of food nutrition literacy was higher for the children who often had fruits at home, rarely ate out, eating without screen, and communicated food and nutrition information with families frequently(P<0.05). Conclusion The food and nutrition literacy of school-age children is not high, which is related with individual and family’s demographic characteristic, nutrition education experience in school and family food environment. Children in rural areas and younger age, with a large number of family children and poor family economic status and food environment, should be the main target population of nutrition education and nutrition improvement.

Objective To explore the application effects of CDIO mode (Conceive, Design, Implement, Operate) in obstetrics and gynecology nursing teaching in associate to bachelor degree students. Methods The CDIO mode was conducted in 2014 nursing associate to bachelor degree students in one college, and the 177 cases of six classes were chosen and divided into experimental group (class 1, 4 and 6;n=88) and control groups (class 2,3 and 5;n=89) by random number table. The students in control group used the traditional teaching model, and the students of experimental group used CDIO teaching mode. At the end of the course, the two groups were tested by theoretical knowledge questionnaire and clinical skills questionnaire, critical thinking aptitude test scale and satisfaction questionnaire.Results The theoretical and skill scores of experimental group were (86.7±4.5) and (90.3±5.8) higher than (79.1±7.3) and (84.6±6.4) in control group (t=8.326, 6.207;P<0.01). The difference of the two groups was statistically significant (P<0.05). Four dimension of critical thinking aptitude test scale including analysis ability, open mind, thirst for knowledge and finding truth in experimental group was higher than that of control group (P<0.05), but no differences between two groups in confidence, cognition mature degree, systematical ability (P>0.05); the satisfaction of experimental group was 94.45% higher than 77.53% of control group (x2=12.132,P<0.01).Conclusions The CDIO teaching model has good application effects in significantly improving students' scores of theory and skills, increasing students' ability of active thinking and innovativeness, as well as students' satisfaction.

【Objective:】 To understand the current situation of nurses’ professional quality of life in China and its correlation with benefit findings, so as to provide reference for formulating targeted measures to improve nurses’ professional quality of life in China. 【Methods:】 Questionnaire star was used to conduct a cross-sectional survey of 11 924 registered nurses in China from January 2022 to April 2022 using the General Information Questionnaire, the Professional Quality of Life Scale (ProQOL) and the General Benefit Discovery Scale (GBFS). SPSS 22.0 software was used for data analysis. 【Results:】 The scores of various dimensions of nurses’ ProQOL scale were: compassion satisfaction was (32.71±6.81) points, burnout was (27.38±5.20) points, secondary traumatic stress was (28.44±6.67) points, and the total score of GBFS was (100.90±18.04) points. The results of multiple linear stepwise regression showed that the main influencing factors of compassion satisfaction were marital status, position, work unit level and educational background (P<0.05). The main influencing factors of burnout were position, professional title, work unit level and whether there were children to be taken care of at home (P<0.05). The main influencing factors of secondary traumatic stress were education background, marital status, professional title, whether there was an old person to be taken care of at home, whether to take care of COVID-19 patients, whether to be an epidemic support staff and position (P<0.05). Benefit finding independently predicted the three dimensions of nurses’ professional quality of life (P<0.001). 【Conclusions:】 Chinese nurses’ professional quality of life was at a moderate level and it was significantly affected by benefit finding. Attention should be paid to factors such as marital status, work unit level, position, professional title, education background, whether there was an old person to be taken care of at home, whether to take care of COVID-19 patients, so as to improve the level of nurses’ benefit finding, promote their personal growth, provide good job guarantee, and improve Chinese nurses’ professional quality of life.

Objective To assess the potential role of disulfidptosis-related genes (DRGs) in pan-cancer on prognosis and immunity on the basis of bioinformatics approaches. Methods Pan-cancer RNA-seq data, mutation profiles, clinical information, TMB, MSI, stemness scores, and tumor and immune microenvironment data contained in TCGA and various open-source online databases, and multi-group R-language algorithms were used for comprehensive analysis. The expression levels of DRGs at the cellular level were experimentally validated using qPCR. Results LRPPRC, NCKAP1, NDUFS1, and NUBPL had a better prognosis in renal clear cell carcinoma (P<0.001), whereas SLC7A11, NCKAP1, and SLC3A2 had a worse prognosis in hepatocellular carcinoma (P<0.001). TME analysis showed that LRPPRC was negatively correlated with immune cells, stromal cells, and estimated scores in all tumor types. TMB analysis revealed the potential research value of DRGs for PD-1/PD-L1 therapy in pan-cancer. Drug sensitivity analysis showed that SLC7A11 (r=0.454), SLC3A2 (r=0.366), and NCKAP1 (r=0.455) were significantly associated with Kahalide F (P<0.01). Experimental validation demonstrated the overall higher expression levels of GYS1 and NCKAP1 than normal cells in lung adenocarcinoma, colon adenocarcinoma, esophageal squamous carcinoma, and hepatocellular carcinoma (P<0.05). Conclusion Pan-cancer analysis of DRGs indicates that DRGs may serve as important biomarkers for the diagnosis and prognosis of renal clear-cell carcinoma, lung adenocarcinoma, and hepatocellular carcinoma.

Objective: To investigate and summarize the effect of narrative nursing on anxiety and depression in patients undergoing coronary artery bypass grafting, and to provide reference for the formulation of clinical nursing plan. Methods: A total of 61 patients underwent coronary artery bypass grafting were selected. 31 cases of narrative nursing were taken as the research group, and routine nursing was used in the other 30 cases as control group. Depression and anxiety were assessed by Self-rating Depression Scale (SDS) and Self-rating Anxiety Scale (SAS) before, 7 days after and 14 days after surgery. Results: Totally 61 patients were successfully operated and no case died during hospitalization. There was no significant difference in SAS and SDS scores between the 2 groups before surgery. The SAS scores in the study group were 57.9±4.2, 50.8±4.9, 47.1±5.0 on the day of admission,7 days after operation and 14 days after operation, respectively, while those in the control group were 58.4±3.7, 57.9±5.2, 55.7±6.2. There were significant differences between the two groups after intervention (t=5.490, 5.973, P<0.01). The SAS score of the study group decreased 7 days after operation and 14 days after operation compared with that of the admission day (t= 6.125, 9.209, P < 0.01). The SDS scores in the study group were 54.3±4.6, 49.4±4.7, 42.7±4.8 on the day of admission,7days after operation and 14 days after operation, respectively, while those in the control group were 54.1±5.1, 52.3±5.6, 51.6±5.2. There were significant differences between the two groups after intervention (t=2.194, 6.949, P<0.01). SDS scores in the study group decreased 7 days after operation and 14 days after operation compared with the day of admission in this group, with significant differences (t=4.148, 9.715, P < 0.01). Conclusions Narrative nursing is helpful to relieve anxiety and depression in patients undergoing coronary artery bypass grafting.