Enzymatic preparations obtained from young plants and cell cultures of capulin were screened for hydroxynitrile lyase activity. The three week old plants, grown under sterile conditions, were used to establish a solid cell culture. Crude preparations obtained from this plant material were evaluated for the transformation of benzaldehyde to the corresponding cyanohydrin (mandelonitrile). The results show that the crude material from roots, stalks, and leaves of young plants and calli of roots, stalks, internodes and petioles biocatalyzed the addition of hydrogen cyanide (HCN) to benzaldehyde with a modest to excellent enantioselectivity.
Acetonitriles ; metabolism ; Aldehyde-Lyases ; metabolism ; Benzaldehydes ; metabolism ; Biocatalysis ; Cells, Cultured ; Hydrogen Cyanide ; metabolism ; Nitriles ; metabolism ; Prunus ; cytology ; enzymology

No abstract available.
Adamantane ; Humans ; Nitriles ; Pyrrolidines

BACKGROUND: A survey for determining the inventory levels of blood products in hospitals is needed, not only for the effective management of the blood products, but also for controlling and planning of the blood supply in Korea. However, any nation-wide survey has never been reported in Korea. Our aims were to provide the information about the status of the RBCs inventory levels of each blood type in the hospitals, and to suggest guidelines for estimating the inventory levels of hospitals. METHODS: We analyzed the data of the average daily usage, the ideal inventory levels and the minimal inventory levels of RBCs according to the each blood type for 28 sentinel hospitals that participate in the 'blood inventory monitoring system'. This system is a surveillance program for monitoring the usage and inventory levels of blood products. RESULTS: The hospitals showed different levels for the average daily usage, the ideal inventory and the minimal inventory according to each blood type. The average daily usage, the ideal inventory level and the minimal inventory level of blood type A were the highest compared to those of the other blood types. For each blood type, the average ideal inventory level is about 5 times higher than the average daily usage, and the average minimal inventory level is about 2 times higher than the average daily usage. CONCLUSION: This is the first nation-wide report on the average daily usage, the ideal inventory levels and the minimal inventory level of each blood type for Korean hospitals, and this data will be helpful to understand the inventory status and estimate the inventory levels of blood products.
Erythrocytes ; Korea ; Nitriles ; Pyrethrins

A sentinel surveillance system for communicable diseases, was implemented in Hung Ha district (Thai Binh province) and Cai Lay district (Tien Giang province), by strengthening the existing routine surveillance system: improvement of microbiological investigation and introduction of computerized reporting and information system.
Communicable Diseases ; Nitriles

No abstract available.
Lymph Nodes ; Nitriles ; Pyrethrins

No abstract available.
Melanoma ; Nitriles ; Pyrethrins

OBJECTIVE: To determine the bonding strength of 2-octyl cyanoacrylate (Dermabond) compared with N-Butyl-2-cyanoacrylate (Histoacryl) and nylon 10-0 (Alcon,) in sealing experimentally induced corneal perforations in cadaver porcine eyes. METHODS: This is a single-blind, randomized, physical experimental study involving 78 freshly enucleated porcine eyes in which perforations of 3.0 and 5.1 mm were made in the cornea and randomly sealed with either interrupted nylon 10-0 (n=13), Dermabond (n=13), or Histoacryl (n=13). Intraocular pressures were raised by injecting normal saline into the anterior chamber and postsealing leaking pressures were measured using a precalibrated manometer attached to the anterior chamber maintainer. Fishers Exact Test was used to determine the difference in proportion of eyes that leaked, and Wilcoxon signed ranked test to compare the mean leaking pressures. RESULTS: In the 3.0 mm group, the proportion of eyes that leaked in Dermabond (2/13, 15.4 percent) and Histoacryl (1/13, 7.7 percent) were comparable (p=1.00). Proportion of leak in nylon 10-0 (13/13, 100 percent) was significantly higher (p0.001). Mean leaking pressures of Dermabond (79.5 mm Hg) and Histoacryl (88.0 mm Hg) were higher compared with that of nylon 10-0 (61.44 mm Hg) (p 0.05). In the 5.1 mm group, proportion of eyes that leaked in Dermabond (4/13, 30.8 percent) and Histoacryl (2/13, 15.4 percent) were comparable (p=0.07 AND P=0.10). CONCLUSION: The bonding strengths of Dermabond and Histoacryl are comparable and greater than that of nylon 10-0. Both are effective for 3.0 mm and 5.1 mm corneal perforations.
Animal ; CYANOACRYLATES ; ENBUCRILATE ; CORNEAL PERFORATION

No abstract available.
Humans ; Nitriles ; Pyrethrins ; Stomach Neoplasms

Ten compounds were isolated from the 70% ethanol extract of linseed meal (Linum usitatissimum L) through a combination of various chromatographic techniques, including silica gel, macroporous adsorbent resin, Sephadex LH-20, and preparative HPLC. On the basis of spectroscopic data analysis, they were elucidated as 1-methylethyl-2-O-beta-D-glucopyranosyl-(1" --> 6')-beta-D-glucopyanoside (1), linustatin (2), neolinustatin (3), lotaustralin (4), linamarin (5), deoxyguanosine (6), deoxyadenosine (7), (+)-pinoresinol-4'-O-beta-D-glucopyranoside (8), 4-O-beta-D-glucopyranosylvanillyl alcohol (9) and tachioside (10), separately. Among them, compound 1 is a new compound, and compounds 6, 8 and 10 were isolated from the linseed meal for the first time.
Amygdalin ; analogs & derivatives ; chemistry ; isolation & purification ; Deoxyadenosines ; chemistry ; isolation & purification ; Deoxyguanosine ; chemistry ; isolation & purification ; Flax ; chemistry ; Glucosides ; chemistry ; isolation & purification ; Glycosides ; chemistry ; isolation & purification ; Lignans ; chemistry ; isolation & purification ; Molecular Structure ; Nitriles ; chemistry ; isolation & purification ; Plants, Medicinal ; chemistry ; Seeds ; chemistry

No abstract available.
Nitriles ; Pyrethrins ; Sentinel Lymph Node Biopsy