This study is to investigate whether naked plasmid DNA can effectively transfect lung cancer related cells and express human kallistatin, an endogenous protein that inhibits angiogenesis and tumor growth, and to explore the biological activity of the low-level expressed kallistatin to lung cancer in vitro and in vivo. The plasmids were delivered with Lipofectamine 2000 to transfect various lung cancer related cells. Kal expression was determined by ELISA. The biological effects of Kal expression on proliferation, migration and apoptosis rate of the cells were examined. In subcutaneous NCI-H446 xenograft model, pKal was injected directly into tumors, the changes of CD34, Ki-67 and E-cadherin expression were detected with immunohistochemical assay, the tumor apoptosis was analyzed with TUNEL assay. Both the endothelial cell and lung cancer cells could express kallistatin after plasmid transfection. The proliferation and migration of human umbilical vein endothelial cells were inhibited, but the apoptosis rate was not affected. The proliferation rates of all the three tested lung cancer cells, such as NCI-H446, NCI-H460 and A549, were inhibited, and their apoptosis rates were enhanced, but different cells behaved differently. In subcutaneous NCI-H446 xenograft model, intratumor injection of pKal inhibited the growth of lung cancer by reducing angiogenesis and proliferation of tumor cells. In conclusion, this study demonstrated the efficacy of plasmid-mediated expression of kallistatin to lung cancer related cells, thus providing a basis for their clinical application in the treatment of lung cancer.

Objective:To evaluate the effect of autologous platelet plasmapheresis (APP) and reinfusion on acute kidney injury (AKI) after total aortic arch replacement.Methods:Sixty American Society of Anesthesiologists physical status Ⅲ or Ⅳ patients of both sexes, aged 18-64 yr, with body mass index of 19-34 kg/m 2, scheduled for total aortic arch replacement under general anesthesia, were divided into 3 groups( n=20 each)using a random number table method: APP-reinfusion group (group A), acute normovolemic hemodilution (ANH) group (group N) and autologous blood withdrawal-reinfusion group (group C). In group A, APP was completed before the operation, concentrated red blood cells were transfused timely according to the intraoperative conditions, and platelet-poor and platelet-rich plasma were transfused after protamine neutralization.In group N, ANH was completed before the operation, and the whole blood released was transfused after protamine neutralization.Autologous blood withdrawal and washed red blood cell (RBC) transfusion were only performed in group C, and autologous blood withdrawal was performed in group A and group N. Cervical venous blood and urine samples were collected at 10 min after anesthesia induction (T 1), at 5min after cardiopulmonary bypass (T 2), immediately after the end of surgery (T 3), at 24h and 48h after surgery (T 4、5) for determination of the concentrations of hematocrit (Hct), plasma free hemoglobin (fHb), urinary neutrophil gelatinase-associated lipocalin (NGAL), tissue metalloproteinase-inhibitor -2 (TIMP-2) and insulin-like growth factor binding protein-7 (IGFBP-7) and for calculation of [TIMP-2]×[IGFBP-7]. Glomerular filtration rate (GFR) and concentrations of reatinine (Scr), urea, uric acid and cystatin C were measured before surgery, on the right postoperative day, and at 1, 2 and 3 days after surgery.The intraoperative requirements for allogeneic blood product transfusion, postoperative mechanical ventilation time, duration of intensive care unit (ICU) stay and hemodialysis were recorded. Results:Compared with group C, the postoperative requirement for allogeneic RBC transfusion in group A and group N and the allogeneic platelet transfusion in group A were significantly reduced, Hct at T 2 in group N was decreased, fHb at T 3 and T 4 in groups N and group A was decreased, Scr concentration on the right postoperative day and at 1 day after operation in group A was decreased, and cystatin C concentration on the right postoperative day and at 2 and 3 days after operation in group A was decreased ( P<0.05). Compared with group N, the postoperative requirement for the allogeneic platelet transfusion, concentrations of NGAL and TIMP-2 at T 3-5, IGFBP-7 concentration and [TIMP-2]×[IGFBP-7] at T 3 and T 4, Scr concentration on the right postoperative day and at 1 day after operation and cystatin C concentration on the right postoperative day and at 2 and 3 days after operation were significantly decreased in group A ( P<0.05). Conclusion:APP-reinfusion provides better efficacy in alleviating postoperative AKI than ANH in the patients undergoing total aortic arch replacement.

The work explored the DEHP migration parameters in PVC infusion in clinic,based on the previous research on the test model of DEHP migrated from PVC infusion,to assess the safety of PVC infusion.The leaching solution samples in different conditions were evaluated by analysis of the DEHP in leaching solution using GC-MS under simulated clinical transfusion way.The release behavior of DEHP was significantly affected by the storage time,storage temperature,surrounding temperature,dripping speed,sterilization process,volume of the leaching solution,and the property of the leaching solution.
Diethylhexyl Phthalate ; pharmacokinetics ; Gas Chromatography-Mass Spectrometry ; Plasticizers ; pharmacokinetics ; Polyvinyl Chloride ; pharmacokinetics ; Temperature