OBJECTIVE:To observe the hypoglycemic effects of Huangsang oral solution.METHODS:Diabetes models of mice was induced with alloxan then the mice were randomly divided into the NS group,model control group,phenformin group,and high dose,middle dose and low dose Huangsang oral solution groups(30.0 g?kg-1,15.0 g?kg-1 and 8.0 g?kg-1).The corresponding drugs were given by ig bid for 10 days.Blood glucose was measured with glucose oxidas method.The effects of Huangsang oral solution on blood glucose concentration and glucose tolerance in diabetic mice as well as on blood glucose concentration in the normal mice were observed.RESULTS:The different dose of Huangsang oral solution could decrease the blood glucose level significantly and improve glucose tolerance in alloxan-induced diabetes mice,and the effect was significant compared with the model control group(P

Objective To study the pregnancy opportunity,treatment of complications,time of ending pregnancy and treatment during and after pregnancy in patients with systemic lupus erythematosus(SLE).Method Prospective study on pregnant patients with SLE was carried out between 2000 and 2003 in Qingdao Municipal Hospital.There were total 29 patients enrolled,all which didn't have cytotoxic drugs at least six months before conception,among whom 23 patients'disease were under control over one year and had low-dose glucocorticoids before pregnancy,two had disease activity under control by mid-and high-dose glucocorticoids within one year and have been pregnant without the direction of the doctor,four new onset during the pregnancy.Results All patients had flares during pregnancy or had multi-complications,10 cases had pregnancy hypertension syndrome,six cases had heart failure during the late pregnancy,three had extensive interstitial lung disease during the late pregnancy.All patients stopped the pregnancy at 30~37 weeks,the total 29 live infants had neither neonatal lupus nor maternal-fetal death.Conclusion The maternal-fetal safety can be increased significantly when disease activity was controlled over one year.Glucocorticoids should be applied when disease flared during pregnancy and postpartum is the key of success,Stop as pregnancy when there are complications in order to improve maternal-fetal safety.

OBJECTIVE: To investigate the relationship between single nucleotide polymorphism of gene and ischemic stroke and search for predisposing genes of ischemic stroke.DATA SOURCES: The Medline database was searched for articles of gene polymorphism of fibrinogen and ischemic stroke published from 1980 to 2005 with the key words of "stroke, plasma fibrinogen, polymorphism"in English. Meanwhile, Vip Information Database was also searched for literatures of gene polymorphism of fibrinogen and schemic stroke published between 1995 and 2005 with the key terms of "ischemic stroke, fibrinogen,gene polymorphism" in Chinese. Full-texts were gotten by correspondence with the authors.STUDY SELECTION: Included literatures were only case control study literatures. Literatures of contrast study, experience summary, individual case and so on were excluded.DATA EXTRACTION: Totally 50 articles of gene polymorphism of fibrinogen and ischemic stroke were retrieved. Twenty articles were accorded with inclusive criteria, and thirty articles were excluded.DATA SYNTHESIS: Level of plasma fibrinogen was co-affected by heredity and environment. Gene polymorphism of fibrinogen has important regulatory effects on the level of plasma fibrinogen. The fibrinogen accelerates thrombotic formation. As a result, the incidence rate of ischemic stroke increased by participating atherosclerosis, helping thrombocytic aggregation and changing the status of hemorheology. But definite mechanism and molecular function of gene polymorphism of fibrinogen deserved further research.CONCLUSION: The relationship between single nucleotide polymorphism of fibrinogen and ischemic stroke remains obscure. It also needs further study.

Food Safety ; International Cooperation ; Risk Assessment

There is controversy about the basic principle of medical ethics in academia field.The autonomy principle has been approved the most widespread.The autonomy principle respects individual autonomy and freedom.Its core is the respect human right,it includes some concrete rules such as informed consent,confidentiality,privacy.The autonomy principle originates from liberalism morals tradition emphasizing on personal freedom and choice.Although it is advocated by western medical ethics,Chinese ancient sage had put forward the colse and even the same viewpoint.

Abdomen ; surgery ; Acupuncture Therapy ; Adolescent ; Adult ; Aged ; Aged, 80 and over ; Child ; Combined Modality Therapy ; Female ; Humans ; Lipid Mobilization ; Male ; Middle Aged ; Pain, Postoperative ; metabolism ; therapy ; Postoperative Period ; Short-Wave Therapy ; Young Adult

Purpose:To study if tamoxifen (TAM) can induce growth arrest and apoptosis of ER negative MA782 mouse breast cancer cell line and to explore the molecular mechanisims. Methods:MA782 cells were cultured in RPMI 1640 medium with TAM.The proliferative activity of cells was detected by MTT methods, and cells apoptosis by flowcytometric methods. The expression of cyclin D1, CDK4 and TGF ?1 proteins was detected by immunohistochemical methods, the semi quantification of protein expression was analyzed by pathological image analysis software.Results:TAM can induce growth arrest and apoptosis of cells. ICC results showed that MA782 cells were ER negative. There was no change of cell cycle regulators in cells with 2 ?mol/L TAM. After 48、72h with 6 ?mol/L or 10 ?mol/L TAM, the level of cyclin D1 proteins decreased from 132.5?0.02 to 129.67?0.03、126.18?0.03(6 ?mol/L) and 109.1?0.01、73.56?0.02(10 ?mol/L),CDK4 proteins decreased from 107.2?0.01 to 91.23?0.02、76.21?0.03(6 ?mol/L)and 83.52?0.02、72.03?0.01(10 ?mol/L), while TGF ?1 proteins increased from 59.72?0.02 to 83.2?0.04、121.75?0.03(6 ?mol/L)and 96.83?0.02、139.01?0.05(10 ?mol/L),The difference was significant( P

Objective: To amplify two human mutant CD59 eukaryotic expressing systems and investigate mutant CD59 functional activity. Methods: Mammalian expression vector PLATER of mutant CD59 cDNAs was transfected into CHO together with the pcDNA by lipofectamine,which confered resistance to G418(400 ?g/ml). The positive clones were tested by FIH. Activity of both mutants CD59 was determined by BCECF release assay. Results: Mutant CD59 cDNAs subcloned into the mammalian expression vector PLATER and transfected CHO together with the pcDNA,which confered resistance to G418. The positive clones were tested by FIH.Activity of both mutants CD59 before and after glycation was determined by BCECF release assay,both of them could restrict MAC formation ,and glycation could inhibit CD59. Conclusion: A eukaryotic system that expressing mutant CD59 cDNA was successfully set up.It was found that mutant CD59 could restrict MAC formation,and glycation could inhibit mutant CD59. These would be helpful for the furthur study of link mutant CD59 and the vascular proliferative of diabetes.

Objective To review the biologic characteristics and biologic effect of cholecystokinine (CCK) on the central nervous system. Methods The literatures of recent years on research advancement of cholecystokinine as neurotransmitters/peptides in signal transduction, neuron protection and pain management in the central nervous system are reviewed. Results CCK possesses the ability to suppress the convulsant effects of convulsants. CCK8 is able to reduce the neural damage caused and delay the neural aging. CCK antagonists play an important role in human pain transduction. Conclusion CCK has been proven to be one of the richest neurotransmitters/neuropeptides as well as an important signal factor in the brain, and its important biologic effect is being confirmed.

Opioid dependence is based on compensatory adaptations in neurons following chronic exposure to opioids. Recent researches show antagonists of N methyl D aspartate (NMDA) receptors inhibit physical and psychic dependence on opioids. This review focuses on the interactions between opioid receptor system and NMDA receptor system, and on the molecular mechanisms of NMDA receptor contributing to opioid dependence. The clinical prospects of NMDA receptor antagonists and related substances on preventing and treating opioid dependence are aslo discussed.