1.Status of libraries and databases for natural products at abroad.
China Journal of Chinese Materia Medica 2015;40(1):29-35
For natural products are one of the important sources for drug discovery, libraries and databases of natural products are significant for the development and research of natural products. At present, most of compound libraries at abroad are synthetic or combinatorial synthetic molecules, resulting to access natural products difficult; for information of natural products are scattered with different standards, it is difficult to construct convenient, comprehensive and large-scale databases for natural products. This paper reviewed the status of current accessing libraries and databases for natural products at abroad and provided some important information for the development of libraries and database for natural products.
Animals
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Biological Products
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chemistry
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pharmacology
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Databases, Factual
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Drug Design
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Humans
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Internet
2.Advances in natural products that target the tumor microenvironment
Ling LI ; Zhe WANG ; Ning-hua TAN
Acta Pharmaceutica Sinica 2021;56(6):1580-1590
The tumor microenvironment (TME), a dynamic and complex local environment, interacts with the tumor cells and is closely related to tumor growth, metastasis, immune escape and drug resistance. Thus, targeting the TME has been a worldwide focus in cancer therapy. Many natural products possess the advantages of multiple targets, multiple pathways and wide pharmacological functions, and are the main source of antitumor drugs. In recent years studies have found that some natural products had advantageous effects on the TME. In this review, we summarize the components and functions of the TME and some natural products that target the TME, with references to the drug therapy of cancer.
4.Twelve benzene derivatives from Clausena excavata.
Wen-Wen PENG ; Wei-Wu SONG ; Mao-Bo HUANG ; Guang-Zhi ZENG ; Ning-Hua TAN
Acta Pharmaceutica Sinica 2014;49(12):1689-1693
A new phenethanol, (2'R)-4-(2', 3'-dihydroxy-3'-methyl-butanoxy)-phenethanol (1), along with other eleven known benzene derivatives (2-12) were isolated from the roots, stems and leaves of Clausena excavata (Rutaceae). Compounds 3 and 4 are new natural products, and compounds 5-8, 10-12 were isolated from C. excavata for the first time. Their structures were elucidated on the basis of MS, 1D and 2D NMR spectroscopic analyses including HSQC, COSY and HMBC experiments. 1 was tested for its cytotoxicities against A549, HeLa and BGC-823 cancer cell lines, and antimicrobial activities against Candida albicans and Staphylococcus aureus. The results showed that 1 did not exhibit cytotoxic and antimicrobial activities.
Benzene Derivatives
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chemistry
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Candida albicans
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drug effects
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Cell Line, Tumor
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Clausena
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chemistry
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HeLa Cells
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Humans
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Magnetic Resonance Spectroscopy
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Molecular Structure
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Plant Leaves
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chemistry
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Plant Roots
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chemistry
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Plant Stems
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chemistry
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Staphylococcus aureus
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drug effects
5.Flavone C-glycosides from seeds of Ziziphus jujuba var. spinosa.
Ke-xian CHEN ; Li-mei ZHAO ; Chang-jiu JI ; Ning-hua TAN
China Journal of Chinese Materia Medica 2015;40(8):1503-1507
Five flavone C-glycosides were isolated from the methanol extract of the degrease seeds of Ziziphus jujuba var. spinosa though various column chromatography methods including silica gel, MPLC, and HPLC. The structures were elucidated as 6"-feruloyl- 6'''-vanillylspinosin(1), 6",6'"-diferuloylspinosin(2), spinosin(3), swertisin(4) and isoswertisin(5) based on the NMR and MS spectral data. 1 is a new compound.
Drugs, Chinese Herbal
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chemistry
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isolation & purification
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Flavones
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chemistry
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isolation & purification
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Glycosides
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chemistry
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isolation & purification
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Magnetic Resonance Spectroscopy
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Mass Spectrometry
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Molecular Structure
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Seeds
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chemistry
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Ziziphus
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chemistry
6.New dicyclopeptides from Dianthus chinensis.
Jing HAN ; Zhe WANG ; Yu-Qing ZHENG ; Guang-Zhi ZENG ; Wen-Jun HE ; Ning-Hua TAN
Acta Pharmaceutica Sinica 2014;49(5):656-660
One new dicyclopeptide cyclo-(L-N-methyl Glu-L-N-methyl Glu) (1), together with one new natural dicyclopeptide cyclo-(L-methyl Glu ester-L-methyl Glu ester) (2), and two known dicyclopeptides cyclo-(L-methyl Glu ester-L-Glu) (3), and cyclo-(L-Glu-L-Glu) (4), were isolated from the aerial parts of Dianthus chinensis L. Their structures were determined by spectroscopic analyses and chemical methods.
Dianthus
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chemistry
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Magnetic Resonance Spectroscopy
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Molecular Structure
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Plant Components, Aerial
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chemistry
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Plants, Medicinal
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chemistry
7.Chemical constituents from stems and leaves of Micromelum integerrimum.
Yan LIU ; Zhi-yao WANG ; Wen-jun HE ; Ning-hua TAN ; Zhi-qi YIN
Acta Pharmaceutica Sinica 2015;50(4):475-479
A new benzene derivative microintegerrin C (1) and a new norsesquiterpenoid microintegerrin D (2), along with six known compounds (3-8), were isolated and identified from stems and leaves of Micromelum integerrimum by various chromatographies such as silica gel, Sephadex LH-20, RP-18 column chromatography and HPLC. Their structures were mainly identified based on the spectral data analysis such as 1D-, 2D-NMR and HR-EI-MS. All known compounds were isolated from this plant for the first time.
Chromatography, High Pressure Liquid
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Plant Leaves
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chemistry
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Plant Stems
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chemistry
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Rutaceae
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chemistry
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Sesquiterpenes
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isolation & purification
8.Monoterpenes and sesquiterpenes from Clausena excavata.
Wen-Wen PENG ; Wei-Wu SONG ; Mao-Bo HUANG ; Ning-Hua TAN
China Journal of Chinese Materia Medica 2014;39(9):1620-1624
To investigate monoterpenes and sesquiterpenes of the stems and leaves of Clausena excavata, an AcOEt fraction of the methanol extract was subjected on column chromatographies including silica gel and RP-18, as well as preparative HPLC. The structures of compounds isolated were identified on the basis of spectroscopic data as excamonoterpene (1), (6R, 9S)-9, 10-dihydroxy-4-megastigmen-3-one (2), (3R, 6R, 7E) -3-hydroxy-4, 7-megastigmadien-9-one (3), (3S) -3-hydroxy-7, 8-dihydro-beta-ionone (4), (3S, 5R, 6S) -3-hydroxy-5,6-epoxy-beta-ionone (5), (6R, 9R) -9-hydroxy-4-megastigmen-3-one (6), (3S, SR) -dihydroxy-6, 7-megstigmadien-9-one(7), (-)-loliolide(8), caryolane-1, 9alpha-diol(9) and 2, 6-dihydroxyhumula-3 (12), 7 (13), 9(E)-triene (10), were isolated from the stems and leaves of C. excavata. Compound 1 is a new monoterpene, named as excamonoterpene. Compounds 2-10 were isolated from this plant for the first time.
Chromatography, High Pressure Liquid
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methods
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Clausena
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chemistry
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Magnetic Resonance Spectroscopy
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Methanol
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chemistry
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Molecular Structure
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Monoterpenes
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analysis
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chemistry
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Plant Leaves
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chemistry
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Plant Stems
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chemistry
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Sesquiterpenes
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analysis
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chemistry
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Spectrometry, Mass, Electrospray Ionization
9.Myricetin attenuates renal fibrosis by activating Nrf2/HO-1 pathway to inhibit oxidative stress
Dong-xue LI ; Zhou HUANG ; Han-yu WANG ; Zhi-hao ZHANG ; Ning-hua TAN ; Xue-yang DENG
Acta Pharmaceutica Sinica 2024;58(2):359-367
This paper investigates the effect of myricetin (MYR) on renal fibrosis induced by unilateral ureteral obstruction (UUO) and common bile duct ligation (CBDL) in mice and its mechanism. The animal experiment has been approved by the Ethics Committee of China Pharmaceutical University (NO: 2022-10-020). Thirty-five ICR mice were divided into control, UUO, UUO+MYR, CBDL and CBDL+MYR groups. H&E and Masson staining were used to detect pathological changes in kidney tissues. Western blot (WB) was used to detect the expression of fibrosis-related proteins in renal tissue, and total superoxide dismutase (SOD) activity detection kit (WST-8) was used to detect the changes of total SOD in renal tissue of CBDL mice.
10.Significance of Respiratory Syncytial Virus-Immunoglobulin M Detection for Diagnosis in Children with Viral Pneumonia
li-ming, TAN ; feng-ying, DUAN ; wei-hua, PENG ; yuan-yuan, WANG ; hua, LI ; ning, LIU ; guo-lin, LI ; wai-mei, WANG
Journal of Applied Clinical Pediatrics 2006;0(16):-
Objective To explore the diagnostic value of detecting respiratory syncytial virus(RSV)-IgM for viral pneumonia in children.Methods Seventy-six cases with interstitisl pneumonia,32 cases with bronchiolitis among 108 cases children with viral pneumonia and 40 healthy children as control group were included,RSV-IgM was detected by indirect immunofluorescence in 108 children with viral pneumonia and healthy control group.Results The positive rates of RSV-IgM in 76 cases with interstitisl pneumonia and 32 cases with bronchiolitis were 48.68% and 25.0%,respectively,there was significant difference between 2 groups(?2=5.20 P