Studies have shown that cholesterol metabolism plays an important role in tumorigenesis and development.At the same time, it is also shown an important application value in predicting the prognosis of malignant tumors.Cholesterol can improve the long-term quality of life and survival of cancer patients by appropriating risk stratification of these malignant tumors.

OBJECTIVE: To study the effect of forsythiaside on the expression of c-jun induced by cisplatin in the cochlea of guinea pig. METHOD: Thirty guinea pigs were randomly divided into control group (10), cisplatin group (10) and forsythiaside group (10). The ototoxicity model was done with intraperitoneal injection of cisplatin solution (8 mg/kg per day) for 7 days. Forsythiaside (25 mg/kg per day) was injected 30 min before cisplatin solution treated in guinea pigs of forsythiaside group for 7 consecutive days. The saline instead of cisplatin was injected in normal control group. The distortion product otoacoustic emission (DPOAE) was detected before animals were killed. The expression of c-jun in cochlea of guinea pigs was detected by western blotting. The expression of c-jun mRNA in cochlea of guinea pigs was detected by reverse transcriptase polymerase chain reaction (RT-PCR). RESULT: DPOAE amplitudes in cisplatin group was significantly lower than in control group (P < 0.01). Compared with cisplatin group, DPOAE amplitudes in forsythiaside group was increased significantly (P < 0.05). The expression of c-jun protein and mRNA were significantly increased in cisplatin group than in control group (P < 0.01). Compared with cisplatin group, the expression of c-jun protein and mRNA were significantly decreased in forsythiaside group. CONCLUSION Forsythiaside can significantly reduce the side effects induced by cisplatin through down-regulating the expression of c-jun.
Animals ; Cisplatin ; toxicity ; Cochlea ; drug effects ; metabolism ; Female ; Glycosides ; pharmacology ; Guinea Pigs ; Male ; Proto-Oncogene Proteins c-jun ; metabolism

Humans ; Medicine, Chinese Traditional ; Nephrotic Syndrome ; diagnosis ; therapy

Objective:To construct recombinant retrovirus expressing human bone morphogenetic protein-7 gene BMP7 and to discuss its apoptosis-inducing activities and the mechanism in liver cancer cell line HepG2. Methods:BMP7 gene was amplified and reconstructed with retroviral plasmid pLP-LNCX by loxP homologous recombination,and then the plasmid pLP-LNCX-BMP7 (pLLBMP7) was transferred into packing cell line PT67 and the supernatant was collected to assay viral titer. MTT assay was adopted to observe HepG2 cells amplification. 48h after pLLBMP7 infection agarose electrophoresis and flow cytometry were used to verify apoptosis of tumor cells,and then the expression of BMP7,caspase-3 and bcl-2 proteins were detected by Western blotting. Results:Recombinant retrovirus pLLBMP7 was justified and transformed into PT67 package cell with supernatant viral titer amounted to 5?109 pfu/ml. In MTT assay retrovirus group had no evident difference from controls in cellular inhibition 72h later (35.1% vs. 5.3%,68.5% vs.18.3%,p

Objective To investigate the pharmacodynamics of sufentanil required to inhibit the body movement induced by tetanic stimulation and skin incision when combined with propofol in patients undergoing thoracic or abdominal surgery. Methods Fifty ASA Ⅰ or Ⅱ patients aged 18-57 yr undergoing elective thoracic or abdominal surgery were randomized into 5 groups sufentanil target effect-site concentration (Ce) (n = 10 each):0.07, 0.10, 0.14, 0.20 and 0.28 ng/ml groups. Anesthesia was induced with TCI of propofol at the target plasma concentration of 3.0-3.2 μg/ml. As soon as the patients lost consciousness, infusion of sufentanil with the corresponding Ce was started in the each group. One tetanic stimulus (frequency 50 Hz, intensity 80 mA, wave length 0.25 ms) was given after the target effect-site and plasma concentrations were balanced. Tracheal intubation was facilitated with succinylcholine 1.5 mg/kg. The concentrations of propofol and sufentanil were maintained until 4 min after skin incision. The body movement was observed during tetanic stimulation and skin incision. The effective effect-site concentration (EC50, EC94) of sufentanil and 95% confidence interval (CI) were calculated using probit regression analysis. Results The EC50 and EC95 of sufentanil required to inhibit the body movement induced by tetanic stimulation when combined with propofol were 0.12 (95% CI 0.09-0.14) ng/ml and 0.20 (95% CI 0.17-0.31) ng/ml respectively. The EC50 and EC95 of sufentanil required to inhibit the body movement induced by skin incision when combined with propofol were 0.13 (95% CI 0.11-0.16) ng/ml and 0.21 (95% CI 0.17-0.29) ng/ml respectively. There was no significant difference in the EC50 and EC95 of sufentanil between the two different stimuli (P ＞ 0.05). Conclusion The EC50 and EC95 of sufentanil required to inhibit the body movement induced by tetanic stimulation (frequency 50 Hz, intensity 80 mA, wave length 0.25 ms) when combined with propofol were 0.12 and 0.20 ng/ml respectively, the EC50 and EC95 of sufentanil required to inhibit the body movement induced by skin incision when combined with propofol were 0.13 and 0.21 ng/ml respectively and there was no significant difference in the pharmacodynamics between the two different stimuli, indicating that tetanic stimulation as an alternative to skin incision can be used to evaluate the pharmacodynamics of anesthetics.

Carcinoma ; diagnosis ; Carcinoma, Papillary ; Diagnostic Errors ; Female ; Humans ; Thyroglossal Cyst ; diagnosis ; Thyroid Neoplasms ; diagnosis ; Young Adult

Objective QR-DVT 9000 NEWTOM CT, an oral maxillofacial dedicated CT, was applied in the diagnosis of maxillary sinus related diseases. Its value and technique are summarized. Method Patients with maxillary sinus related diseases were scanned and images were reconstructed with QR-DVT 9000 CT. The axial view of reconstructed image was analyzed in sagittal plane, coronal plane and 3-D. Results QR-DVT 9000 NEWTOM CT could display the position, shape and anatomic relationship of maxillary sinus with adjacent structures in sagittal image, coronal image and 3-D image. Conclusion QR-DVT 9000 NEWTOM CT, an oral maxillofacial dedicated CT, is considerably valuable in the diagnosis of maxillary sinus related diseases, and it can guide clinical surgery more effectively than routine X ray examination.

OBJECTIVE:To compare clinical efficacy and safety of olanzapine and quetiapine for mental disorders elderly pa-tients. METHODS:120 elderly patients with mental disorders were randomly divided into olanzapine group and quetiapine group, with 60 cases in each group. Olanzapine group was given Olanzapine tablet orally with initial dose of 2.5 mg,qd,and then increas-ing to maximal dose 15 mg according to disease condition,qd;quetiapine group was given Quetiapine tablet orally with initial dose of 50 mg,qd,increasing to maximal dose 400 mg according to disease condtion,qd. Both groups received 6 weeks of treat-ment. Clinical efficacy of 2 groups were compared as well as PANSS and the quality score of life before and after treatment,and the occurrence of ADR. RESULTS:PANSS and the quality score of life were improved significantly in both groups,with statisti-cal significance (P>0.05);but there was no statistical significance between 2 groups (P>0.05). There was no statistical signifi-cance in clinical efficacy between 2 groups(P>0.05). The incidence of lethargy,hypotension and bradycardia in quetiapine group were significantly lower than in olanzapine group,while the incidence of tachycardia was significantly higher than olanzapine group,with statistical significance (P<0.05). CONCLUSIONS:Olanzapine and quetiapine are similar to each other in the treat-ment of mental disorders elderly patients;both of them can effectively improve the quality of life,and ADR induced by quetiapine is slighter than that induced by olanzapine.

Adult ; Female ; Hemoperfusion ; Humans ; Male ; Neurotoxicity Syndromes ; etiology ; therapy ; Organophosphate Poisoning ; Pesticides ; poisoning ; Retrospective Studies ; Treatment Outcome

Food Contamination ; prevention & control ; Public Health Practice