This paper not only analyzes the technology characteristic and market prospects of medical motor vehicle equipment in China,summarizes the current status of the development and standardization of medical vehicles inside and outside army,but also demonstrates the standardization system of medical motor vehicle technology in China,puts forward the solving methods and measures for the lack of medical motor vehicle equipment standards.

The nonstandard phenomenon of national military compiled version and some main question of approval work are summarized briefly, and then including and analyzing the season of them, which can improve the authorized standard and approval work quality, enhance the argumentation on standard of approve and initiatal a project, strengthen authorized work management and develop management tracking and so on.

Objective To study pathological diagnostic value of forceps and brush biopsy cytological examination by percutaneous transhepatic cholangiodrainage(PTCD) for malignant obstructive jaundice.Methods From April 2005 to April 2006,24 cases of obstructive jaundice underwent PTCD and stent insertion in biliary tract.Tissue pathological examination by forceps and brush was done during PTCD.Results 24 cases received forceps biopsy,of whom,14 cases received brush cytological examination at the same time.All of the 24 cases of forceps biopsy obtained pathological tissue and the pathological results as follows: adencarcinoma in 15 cases,4 cases had allotype cells,regarded as positive results;inflammation tissue in 1 case,which was restenosis after stent insertion;fibrous tissue in 1 case;tissue obtained was cholangioepithelia in 3 cases,in which 1 case was diagnosed as lymph node metastasis of peripheral head of pancreas after gastric cancer operation,2 cases were diagnosed as cholangiocarcinoma,the positive rate of forceps biopsy being 79.2%(19/24).4 cases of cytological examinations by brush showed tumor cells in 2 cases,allotype cells in 2 cases,regarded as positive results,the positive rate being 28.6%(4/14),and others were negative results.Conclusions Intracholangio-biopsy by PTCD offers the advantages of simplicity,safety,higher accuracy and less complications.Pathological diagnosis can be obtained at the same time during reducing-jaundice therapy by PTCD.

AIM:To compare the quality of Liuwei Dihuang Pill produced by different pharmaceutical companies by determining the content and the in vitro dissolution of paeonol and loganin of Liuwei Dihuang Pill.METHODS:The HPLC method was used for the assay of paeonol and loganin in Liuwei Dihuang Pill.The standard dissolution test was used to investigate the in vitro dissolution of paeonol and loganin.RESULTS:The content of paeonol and loganin from different manufacturers differed significantly.Six products differed significantly from one another in their in vitro dissolution(P

Objective To set up a RP-HPLC method for determining gastrodin and ferulic acid in drug delivery system of Dachuanxiong simultaneously. Methods ODS-2 Hypersil column was used with methanol-1% glacial acetic acid as solution gradient elution. The flow rate was 1 mL/min and column temperature was 25 ℃. The wavelength of detector of gastrodin was 270 nm, and ferulic acid was 322 nm. Results Gastrodin and ferulic acid can be separated well with other components within 40 minutes. The linear range of gastrodin was 0.092～1.840 ?g (r =1.000 0), and ferulic acid was 0.122～2.440 ?g (r =1.000 0). The average recovery rate of gastrodin was 99.50% with RSD=1.23% (n =5), and ferulic acid was 101.5% with RSD=1.52% (n=5). Conclusion The method is simple, rapid, accurate and reliable, and can be used for determining gastrodin and ferulic acid in drug delivery system of Dachuanxiong simultaneously.

Objective To observe the clinical features of polypoidal choroidal vasculopathy (PCV) in Chinese patients. Methods Nine cases (9 eyes) were examined with fundus examination, fundus fluorescein angiography (FFA) and indocyanine green angiography angiography (ICGA). Results FFA and ICGA showed the branching vascular networks (7 cases) and polyplike dilation at terminals of branches (9 cases), which mainly located in macular area (8 cases) and in peripapillary area (1 case), and which accompanied hemorrhagic or serous pigment epithelial detachment in 7 cases,and 4 of 7 cases had a significant horizontal black-white damarcation line. It definitely differed from fine choroidal neo-vascularization (CNV). Conclusion PCV in Chinese patients has the cardinal clinical features, i.e., polyplike lesions located mainly in macular area and most cases accompanied by hemorrhagic or serous pigment epithelium detachment.

Associating liver partition and portal vein ligation for staged hepatectomy (ALPPS) is a new surgical management which brings hopes of cure to patients with primary or metastatic liver tumor who do not have sufficient future liver remnant (FLR).This review summarizes the current research on the development,indication,surgical procedures and safety of ALPPS.It also discusses the major concerns and unanswered doubts of ALPPS,such as the comparison with selective portal vein embolization (PVE) and the long-term oncological progression.

Adult ; Female ; Hemoperfusion ; Humans ; Male ; Neurotoxicity Syndromes ; etiology ; therapy ; Organophosphate Poisoning ; Pesticides ; poisoning ; Retrospective Studies ; Treatment Outcome

Objective　To obtain mammalian cell expression vector of human CD154 gene. Methods　A 820 bp cDNA fragment was amplified by RT-PCR method from total RNA of human peripheral blood mononuclear cell（PBMC） activated with 10 ng／mL PMA and 1 μg／mL PHA for 8 hours. The fragment was cloned into pcDNA3.1（＋） plasmids.The cloned insert was identified by double digestion of the recombinant plasmid with restriction enzymes BamH Ⅰ and EcoR Ⅰ and sequenced by Sangers-dideory-mediated chain termination. Results　This cDNA fragment included 820 bp entire coding region and a part of the 3 non-coding region. The recombinant mammalian cell expression vector of pcDNA3.1（＋）／hCD154 was constructed， the sequence of the insert was identical to the published sequence encoding human CD154 antigen. Conclusion　The recombinant mammalian cell expression vector of pcDNA3.1（＋）／hCD154 was successfully constructed.

Objective To investigate the role of glutathione (GSH) synthesis in the regulation of Glutamine (Gin) on TNF-α release in lipopolysaccharide (LPS)-stimulated alveolar epithelial type Ⅱ cells (AEC Ⅱ). Method Primary cultured AECⅡ were divided into six groups, including control, 10.0 mmol/L Gln, 1 μg/mL LPS and LPS+(0.5, 2.0 and 10.0 mmol/L) Gln. In another set of experiments, AECⅡ were divided into six groups including 1 μg/mL LPS, LPS+10.0 mmol/L Gln, LPS+100 μmol/L L-buthionine-(S, R)-sulfoximine (BSO), LPS+Gln+(1,10 and 100 μmol/L) BSO. Each group had three samples. BSO and Gln were added at 8 hours before LPS challenge. After 24 hours of LPS stimulation, cells were obtained for GSH measurement by 5, 5'-dithiobis-(2-nitrobenzoic acid) (DTNB) method. TNF-α level in the supematant was determined by enzyme-hnked immunesorbent assay (ELISA). ANOVA (LSD-t test) was used for statistical analysis. Results Supple-mentation of Gin increased the GSH level and attenuated TNF-α release in LPS-stimulated AEC Ⅱ in a dose-depen-dam manner. GSH level increased from (50.69±3.04) pmol/mg cell (LPS group) to (126.74±7.13) pmol/mg cell (LPS+10.0 mmol/L group) (P<0.01) and TNF-α level decreased from (1104.5±48.8) pg/mL (LPS group) to (329.67±48.27) pg/mL (LPS+10.0 mmol/L group) (P<0.01). BSO, an GSH synthesis blocker, at doses greater than 10 μmol/L reversed the effect of Gin significantly (P<0.01). Conclusions As a precursor ofGSH, glutsmine could attenuate TNT-α release in LPS-stimulated AECⅡ,and the with may be mediated via GSH synthesis.