The major target of translational medicine is the rapid application of achievements in basic research to clinical medicine. How to carry out embedded subject service for translational medicine in academic library was thus described in aspects of constructing its information service platform, establishing its service team, and deciding its service methods.

Objective To explore the prevalence and related factors of female sexual dysfunction(FSD) in women with diabetic.Methods Female Sexual Function Index(FSFI) was used to investigate the prevalence of FSD in 336 women with diabetes.Logistic analysis was conducted between diabetic women with FSD and without FsD for related factom of diabetic FSD.Results Two hundred and fourty-four patients were found to have FSD.The prevalence was 72.6% .The Logistic analysis showed that FSD was significantly relaed to diahetic course and diabetic neuropathy.Conclusion The prevalence of FSD in diabetic women is significandy greater than non-diabetic women.Diabetic course and diabetic neuropathy play an important role in the development of FSD in diabetic women.

Animals ; Carcinoma, Non-Small-Cell Lung ; genetics ; metabolism ; Humans ; Lung Neoplasms ; genetics ; metabolism ; Molecular Imaging ; Mutation ; Positron-Emission Tomography ; RNA, Messenger ; metabolism ; Receptor, Epidermal Growth Factor ; biosynthesis ; genetics

Objective Liver metastasis model of human primary gastric lymphoma in nude mice was reproduced for an experiment to evaluate the inhibitory effect of cytokine-induced killer (CIK) cells on the growth and liver metastasis of primary gastric lymphoma. Methods Surgical orthotopic implantation of a histologically intact liver metastasis fragment derived from a surgical specimen of a patient with metastatic gastric lymphoma was initally implanted, in order to reprodueing a liver metastasis model of human primary gastric lymphoma. Peripheral blood mononuclear cells (PBMC), isolated by blood cell separator from healthy donors and patients with primary gastric lymphoma, were incubated in vitro. rhIFN-?, rhIL-2 and anti-CD3 McAb were added to PBMC in order to prepare CIK cells as well as lymphokine-activated killer (LAK) cells. CIK and LAK cells from different donors were used in treating gastric malignant lymphoma, so as to investigate the inhibitory effect of 2 kinds of effector cells on the growth of the tumor and liver metastasis. Results The liver metastasis model of human primary gastric malignant lymphoma in nude mice was successfully reproduced. After administration of different agents continuously for 20 days (0.3ml/d), the inhibitory rates of the following 4 groups, healthy donors LAK group (2?1010/ml), healthy donors CIK group (2?1010/ml), patients CIK group (1?1010/ml) and patient CIK group (2?1010/ml), were 39.28%, 53.57%, 40.38% and 56.42%, respectively. The liver metastasis rates in control group, healthy donors LAK group, healthy donors CIK group, patients CIK group (1?1010/ml) and patient CIK group (2?1010/ml), were 100.0%, 62.5%, 50.0%, 62.5% and 37.5%, respectively. Tumor weights of all treatment groups were lighter than that of saline group (P

Obesity-related diabetes and the metabolic syndrome are significantly related to increased adipose tissue mass.Understanding of the effect of adipose tissue on the metabolic regulation will play a key role in therapeutic strategies aimed to reduce obesity-induced insulin resistance and alleviate symptoms of the metabolic syndrome.Visfatin is a novel adipokine that appears to be preferentially produced by visceral adipose tissue and has insulin-mimetic actions.We discusse the relationship between visfatin and metabolic syndrome as well as future therapeutic strategies to these diseases.

Objective To compare the safety of two antibody inductors, namely lymphocytes scavenger and IL-2 receptor blocking agent, in living kidney transplantation. Methods The data of 191 patients, who received living kidney transplant in our hospital from Feb. 2007 to Jul. 2012, were retrospectively analyzed, and grouped according to the inductors they received as: a) lymphocytes scavenger group (n=56), with rabbit antithymocyte immunoglobulin (rATG, 4 cases) and porcine antihuman T-lymphocyte immunoglobulin (pATG, 52 cases) served as the inductor; b) IL-2 receptor blocking agent group (n=54), with basiliximab (40 cases) and daclizumab (14 cases) served as the inductor; and c) control group (n=8l). The incidence of rejection and infection, and the survival rate of patient/allograft within one year were then compared among the three groups. Results Within one year after the transplantation, the incidence of acute rejection in lymphocytes scavenger group, IL-2 receptor blocking agent group and control group was 12.5%, 11.1% and 28.4%, respectively. There was a significant difference between the two inductor groups and control group (P=0.003), but no significant difference was found between the two inductor groups (P>0.05). The incidence of delayed graft function (DGF) in the three groups was 8.9%, 7.4% and 13.6%, respectively, with no statistical significance (P>0.05). Also there was no significant difference among the three groups in the incidence of infection and the survival rate of patient/allograft within one year after transplantation (P>0.05). Conclusion Both inductors may significantly reduce the incidence of acute rejection within one year without increasing the incidence of infection and other adverse events, nor affect the postoperative patient/graft survival, so they are both safe and effective.

AIM　Diclofenac potassium sustained-release tablets were developed as a gel matrix for using twice daily and evaluated in vitro release characteristics. METHODS　The formulations were screened according to the designed in vitro release rate by the use of HPMC as the gel matrix and the hydrophobic retarding agent to modify the release. RESULTS　The release was properly characterized by the diffusion mechanism, influenced by pH of the media, slightly affected by the basket rotating speed, without the influences from the pressure exerted during the tabletting procedure. CONCLUSION　The selected formulation of diclofenac potassium sustained-release tablets could ensure the desired in vitro release rate. In addition, it proved a good manufacturing reproducibility.

Audiometry, Speech ; Humans ; Language

Objective To study the relationship between sICAM 1,sVCAM 1,TNF ?,TNFR Ⅰ,TNFR Ⅱ and pathogenesis of vasculitis.Method The serum levels of TNF ?,TNFR Ⅰ,TNFR Ⅱ,sICAM 1 and sVCAM 1 were detected by ELISA and their levels between patients with vasculitis and controls were compared.Results Compared with the controls,the serum level of TNF ? was lower,the levels of TNFR Ⅰ,sICAM 1 and sVCAM 1 were higher in the patients with vasculitis,and there was no difference of TNFR Ⅱ between the two groups.Conclusion The low level of TNF ? suggests that this factor may not play a role in these patients,or its role was counteracted by its receptors—sTNFR,whose level was higher in patients with vasculitis.sICAM 1 and sVCAM 1 may play a significant role in the pathogenesis of vasculitis.

Objective To investigate the effect of matrix metalloproteinase(MMP-2) in the attack of exprimental autoimmune myositis(EAM) rats, and the effects of methylprednisolone on expressions of gene mRNA and protein of MMP-2. Methods The expression levels of MMP-2 in the peripheral blood, spleen lymphocytes and muscles were detected by using RT-PCR and immunohistochemistry techniques and was compared among EAM group,EAM with methylprednisolone treatment group (EAMM) and control group. Results ⑴ The degree of episode in EAMM group was lower than that of EAM group , and the infiltration of the inflammatory cells and the necrosis of the muscles were also mild in EAMM group. ⑵There was significant upregulation of the expression of the MMP-2 gene mRNA in the lymphocytes of peripheral bood and spleen of EAM rats as compared with that of the control,Elevation of the expression of MMP-2 protein in rats muscles tissues of EAMM group was observed obviously. However, the expression of the mRNA and the protein of MMP-2 was suppressed significantly in EAM group compared with control group. Conclusions The upregulation of the expression of MMP-2 may be correlated with the onset of EAM. Methylprednisolone may relieve the degree of the pathology of EAM by the downregulation of MMP-2 .