Objective To analyze the factors related to glare occurred after the phakic intraocular lens (P-IOL) implantation,and to raise the standard for the P-IOL selection.Design Retrospective case series.Participants 104 patients with high myopia underwent P-IOL implantation in Beijing Tongren Hospital.Methods In 104 cases,22 cases (22 eyes) were implanted angle supported Phakic6 designed accord with corneal symmetry,54 cases (54 eyes) were implanted iris fixed Verisyse,and 28 cases (28 eyes) were implanted posterior chamber PRL.Selection of the diameter of P-IOL optics depended on P-IOL diopters.The pupil diameter,the P-IOL optic center position,the pupil and corneal center position were measured with anterior segment optical coherence tomography (OCT).The contrast sensitivity (CS) under different condition (day,day and glare,night,night and glare) and in different space frequency (1.5,3,6, 12,18c/d)were examined with Optec6500 system.Main Outcome Measures Glare incidence,CS,and the influence of pupil diameter and P-IOL location to glare.Results None of the 104 P-IOL eyes occurred glare in day time.59 eyes (56.73%) complained of glare in dime condition.The glare incidence was 63.18% (14/22) in Phakic6 eyes,55.56% (30/54) in Verisyse eyes,and 53.27% (15/28) in PRL eyes (P=0.691).CS of all surgical eyes were improved in day time with or without glare luminous environment (P=0.000).And the CS of halo-complaining eyes was lower than of no halo eyes in night time with or without glare luminous environment (P=0.000).Glare oc- curred more frequently in eyes with the pupil diameter larger than P-IOL diameter in clime condition after surgery (10 in Phakic6 eyes, 20 in Verisyse eyes,12 in PRL eyes,all P

Objective: To investigate factors related to alexithymia of patients with essential hypertension or type 2 diabetes mellitus. Method: 42 patients wi th essential hypertension, 40 with diabetes mellitus and 45 healthy control were assessed by TAS (Toronto Alexithymia Scale), SCL-90 and EPQ (Eysenck Personalit y Questionnaire). Multiple correlation analysis and multiple stepwise regressio n were used in data analysis. Result: Compared with normal contr ol, patients with hypertension or diabetes showed alexithymia. They were deficient in the abilit y of describing emotion, recognizing and distinguishing between emotion and body feeling, and used to extroversion thought. Besides these, patients with diabete s showed fancy-lacking either. There was close relation between personality and a lexithymia. The more introversive of the hypertensives, the more deficient they were in describing emotion. The more introversive of the diabetics, the more de f icient they were in recognizing and making distinguish between emotion and body feeling. Hostility ideation and neuroticism of hypertension patients were major factors related to alexithymia. Paranoid ideation and psychoticism had similar e ffects in diabetics. Conclusion: Patients with essential hypert ension or diabete s have alexithymia relating to their personality traits and psychological state .

AIM: To conclude the characteristics of flash visual evoke potentials (FVEP), and pattern visual evoke potentials (PVEP) of the healthy school-age children.And to compare the two methods, in order to find the association of them, and to find the impact of sex, age, and the other biological variables.METHODS: A total of 101 healthy children were recruited (age from 5 to 14.4y, mean 8.27y).Each of them was underwent FVEP and PVEP examinations.Then the results were statistically analyzed by SPSS 13.0.RESULTS: The curves of PVEP are simple and stable, while FVEP waveforms are variable.The latency of P100 of females is shorter than males.However there was no significant difference for FVEP in sex control.To compare the parameters between the two hemispheres, the amplitude of P100 of left eyes were higher than the right side.FVEP showed no difference in the two hemispheres either.There was no significant difference for age-dependent decreased in neither PVEP nor FVEP.And in a regression analysis of the FVEP and PVEP, we could not find the inner connection of the two methods.CONCLUSION: Based on our research, there were no significant differences in age level or sex control in the period of school-age children.And there is no inner connection of the two methods.The differences between the PVEP and FVEP results might be due to the origin of these two responses.And these two stimuli should be used in a complementary manner not as alternative examinations.

Animals ; Cells, Cultured ; Ethanol ; toxicity ; Hepatocytes ; drug effects ; metabolism ; pathology ; Male ; Mice ; Mice, Inbred Strains ; Transcription Factor RelA ; metabolism

Using Vibrio fluvialis Ⅱ as host bacteria, 19 bacteriophages have been isolated from the 76 samples which were collected from Haliotis discus hannai ～growing seawater in Dalian marine culture company Dalian, liaoning province from May in 1996 to August I 1997. Ultrastructure of 19 bacteriophages were observed with electron to Bradley the results showed that of these bacteriophages belonged to Bradley A type, they have hexagonal heads of bacteriophages were identified with VP1,VP2,VP4,VP8 as representatives respectively. The phages remain stable at pH6. 0～10. 0, moreover VP2,VP4 and VP8 are rather stable at basic pHs. Although the characterization of heat inactivation course of VP4 is different from others, four phages are sensitive to heat and inactivated at 80℃ in 5 minutes. One step growth experiment showed that the eclipse period of VP1,VP2,VP4,VP8 are sensitive to heat and the eclipse period of VP1, VP2, VP4, VP8 are 42, 30, 46, 28 minutes. In this experiment we have isolated at least four different types phages, it suggest that in fact there is a population of phages in the seawater environment. The result of this study provided a way to find the potential value of phages as an indicator of pathogenic microorganisms Vibrio fluvilis Ⅱ in marine environment.

OBJECTIVETo study the effect of total saponins from Rhizoma Dioscoreae nipponicae (RDN) on the expression of stroma cell derived factor 1 (SDF1) and IKB kinase (IKK) in rIL-1beta induced fibroblast-like synoviocytes (FLS).

METHODSFLS were primarily cultured and the 3rd generation log phase growth FLS were divided into the normal control group, the model group, and the medication group. 10 microg/L rIL-1beta was used to induce the proliferation of FLS in the model group.10 microg/L rIL-1beta and 100 microg/L RDN were administered to co-incubate FLS in the medication group. No treatment was given to FLS in the normal control group. Expression levels of SDF1 and IkapaB kinase proteins (p-IKK) were detected using Western blot.

RESULTSExpression levels of SDF1 and p-IKK increased significantly higher in the model group than in the normal control group (P<0.01). Compared with the model group, expression levels of SDF-1 and p-IKK significantly decreased in the medication group (P <0.01).

CONCLUSIONSTotal saponins from RDN could inhibit the activation of both SDF1 and p-IKK. It might further regulate the expression of IKB kinase by regulating the expression of SDF1.

Cells, Cultured ; Chemokine CXCL12 ; metabolism ; Dioscorea ; Drugs, Chinese Herbal ; pharmacology ; therapeutic use ; Epithelial Cells ; Fibroblasts ; Humans ; Interleukin-1beta ; metabolism ; Saponins ; metabolism ; Synovial Membrane ; metabolism

Objective To observe the dynamic change of cerebral blood flow of newborns with hypoxic-ischemic encephalopathy(HIE).Methods Cerebral blood flow of middle cerebral artery and pulsatility index(PI) on 75 newborns with HIE and 50 normal infants were examined with transcranial doppler sonography at different time points,and the relations between cerebral blood flow and clinic indexes were analyzed.Results The blood velocity of normal infants increased gradually, and PI decreased from 2 to 5 days.The velocities were lower than that of normal infants,and PI was higher at 12th hour and 1st day, but during 2-5 days,the velocities got higher and PI got lower, in which the decrease of velocities correlated positively with Apgar scores and the increase of velocities were negatively correlative to Apgar scores.Compared with the neonates who had poor prognosis retrospectively with those had good prognosis, the velocity changes were found to be more significant.Conclusion The change of cerebral blood flow can show the pathophysiology of HIE and prognosticate the prognosis of neonates with HIE.

Objective To evaluate the clinical effect of dorsal interosseous artery island flap to cover soft tissue defects of the hand.Methods Ten cases (8 males and 2 females) treated between 2009 and 2012 were included.The age was from 15 to 74 years.There were 4 patients associated with mechanical injury,3 electric injury,2 chronic ulcer and 1 hot crush injury.Size of the defect ranged from 3.0 cm × 2.5 cm to 12.0 cm × 7.0 cm,and the size of the flap ranged from 4.0 cm× 3.0 cm to 13.0 cm × 8.0 cm.The portable Doppler ultrasound was employed to locate the branches from the forearm dorsal interosseous artery.The dorsal interosseous retrograde artery distally based was used to repair hand softtissue defects.Results All the flaps survived except for some necrosis in one case which healed after dressing change.Eight patients were followed up for 3 months to 1 year and two patients were lost to follow-up.Flap had soft texture and similar color and sensation as the adjacent skin.No apparent hypertrophic scarfing on the donor site was observed.Flap was slighdy swollen in one case.Conclusion Dorsal interosseous artery retrograde island flap is safe and effective to repair hand soft tissue defects.

Objective To explore the proliferation of microglia in the frontal cortex,hippocampus and amygdala of the post-stroke depression (PSD) in rats.To understand the role of microglia in the pathogenesis of PSD.Methods The adult female SD rats were divided into four groups(n=5 per group):normal control group,depression group,stroke group and PSD group.In the depression group,the depression model rat were established by chronic unpredictable mild stress (CUMS) combined with separately breeding.In the stroke group,focal cerebral ischemic rat models were established with thread embolization method.In the PSD group,focal cerebral ischemic models rat were established with thread embolization method firstly,and then PSD models rat were established with comprehensive chronic unpredictable mild stress(CUMS) and separately breeding on this basis.After the procedure,rats were subjected to sucrose preference test and open field test.At the postoperative eighth weekend,immunofluorescence technology was used to detect the proliferation changes of OX42 positive cells in the frontal lobe,hippocampus and amygdale.Results At the 29th day after CUMS,the sucrose solution consumption ((23.8±0.8) ％),horizontal movement distance of open field test ((63.0± 1.2) cm) and vertical movement distance ((25.0± 1.0) cm) in PSD group were significantly lower than those in normal group((31.2± 1.9) ％;(69.8±2.3) cm;(31.0± 1.6) cm) and depression group((31.0±1.4) ％;(70.2±2.4) cm;(30.8± 1.1) cm) (P＜0.05).The number of OX42 positive cells of frontal lobe,hippocampus and amygdale in PSD group((20.8±2.6);(20.2±1.3);(19.8±2.6))increased significantly compared with those of normal group((7.4±2.3);(8.0± 1.6);(9.4±2.1)),depression group((8.0±2.0);(7.8 ±2.2);(9.2±1.9))and stroke group((9.6±1.1);(9.4±2.2);(10.2±2.6)) (all P＜0.05).Conclusion The number of microglia in PSD group in the emotional disorders related brain areas(the frontal lobe,hippocampus and amygdale) increases obviously and the increased expression of microglia in the emotional disorders related brain areas may be responsible for the pathogenesis of PSD.

Objective To explore the expression of the CNPase positive oligodendrocytes in the frontal cortex,hippocampus and amygdala of the post-stroke depression (PSD) model rats,and to understand the role of oligodendrocytes in the pathogenesis of PSD.Methods Healthy adult SD rats were randomly divided into normal group,depression group,stroke group,and PSD group (n =5 in each group).In the stroke group,focal cerebral ischemic rat model was made with thread embolization method.In the depression group,the depression model rats were made by chronic unpredictable mild stress(CUMS) combined with separately breeding.In the PSD group,focal cerebral ischemic rat model was made with thread embolization method firstly,and then PSD rat model was established with comprehensive chronic unpredictable mild stress (CUMS) and separately breeding on this basis.After the procedure,rats were subjected to sucrose preference test (SPT) and open field test (OFT).Immunofluorescence staining was used to detect the expression of the CNPase positive oligodendrocytes in the frontal cortex,hippocampus and amygdala at day 29.Results On the 29th day after CUMS,comparing the sucrose solution consumption,horizontal movement distance of open field test and vertical movement distance of the each group,the depression((26.6± 1.1)％,(63.6±2.3)cm,(26.4±1.1)cm) and the PSD groups((23.8±0.8)％,(63.0± 1.2) cm,(25.0± 1.0) cm) were significantlylower than normal ((31.2± 1.9) ％,(69.8± 2.3) cm,(31.0 ± 1.6) cm) and the stroke groups ((31.0± 1.4) ％,(70.2±2.4) cm,(30.8 ± 1.1) cm) (P＜ 0.05).Comparing the expression of the CNPase positive oligodendrocytes in the frontal cortex,hippocampus and amygdala of the each group,the number of the stroke((16.60± 1.82),(14.60±1.82),(15.00±2.12)),depression((16.40±2.07),(14.80±2.17),(15.80±2.28)) and the PSD groups((12.40± 1.52),(11.20± 1.48),(10.80± 1.92)) were significantly less than that in normal group((20.40±3.51),(18.20±2.59),(19.00±2.55)),and the number of expression CNPase positive oligodendrocytes in the PSD group was significantly less than that in stroke and depression groups(all P＜0.05).Conclusion The number of the CNPase positive oligodendrocytes in PSD group in the emotional disorders related brain areas (the frontal lobe,hippocampus and amygdale) reduced obviously,and the oligodendrocytes may play an important role in the pathogenesis of PSD.