Objective To explore the relationships among personality,coping style and clinicians' job burnout.Methods 1569 clinicians were investigated with Chinese Maslach Burnout Inventory,Eysenck Personality Questionnaire and Simple Coping Style Questionnaire.The data were performed with regression and path analysis.Results The proportion of burnout was 72.4％,the proportion of slight burnout was 36.5 ％,the proportion of moderate burnout was 31.2％ and the proportion of high burnout was 4.7％.When taking demographic variables into consideration,more introvert and neuroticism indicated increasing tendency of emotional exhaustion.More psychoticism,neuroticism and negative coping style indicated increasing tendency of depersonalization and more introvert and negative coping style indicated increasing of reduced personal accomplishment.Coping style could partly mediate the relationship between personality and job burnout.Conclusion Chinese clinicians suffer from burnout severely,coping styles mediate between the personality factors and the burnout level.

Objective This article aims to investigate the effect of gender determination for measuring the 12th Thoracic Vertebra by using computed tomography (CT) and explore the feasibility for the research of human skeleton measuring by CT technique in the ifeld of forensic anthropology.Methods After ascertaining 11 radial line indicators,we measured Multi-planner Reformation (MPR)image of the 12th Thoracic Vertebra. Four ratio indicators were calculated after eliminating measurement uncertainty of results. We inspected the effect of gender determination for measuring the 12th Thoracic Vertebra by using computed tomography (CT) on account of statistical measurement data.Results Gender determination of 12 indicators is remarkable after eliminating 3 unstable indicators. Sevene quations were established. The equation established by centrum indicators predicted sex up to 91.6% accuracy.Conclusion The morphometry indicators of 12th Thoracic Vertebra have gender difference. CT technology can accurately measure skeleton and is valuale for study of human skeleton measuring research in the ifeld of forensic anthropology.

Objective This article aim to optimize facial characteristic indexes and to establish the standard of classiifcation by studying positive photos of Chinese Han nationality adults, and to investigate the personal identiifca-tion power ifnally. Methods Firstly, we determined the 27 facial characteristic indexes and standard of classiifcation. Secondly, we observed 254 positive photos of Chinese Han nationality adults. Finally, indexes and standard of classi-ifcation are evaluated through statistically analysising of observation data results and calculating personal identiifcation capability. Results Thirteen indexes gender differences are signiifcant. All 27 facial characteristic indexes are calculated. Male TDP(total discrimination power)=0.999 999 909 08, female TDP=0.999 999 919 82, overall TDP=0.999 999 868. Conclusion The more facial characteristic indexes and the more classiifcation and the higher degree of classiifcation, the higher personal identiifcation ability can be acquired.

Active Transport, Cell Nucleus ; Capsid Proteins ; physiology ; Heparan Sulfate Proteoglycans ; physiology ; Humans ; Oncogene Proteins, Viral ; physiology ; Papillomaviridae ; genetics ; physiology ; Papillomavirus Infections ; etiology ; Viral Structural Proteins ; physiology ; Virion ; physiology ; Virus Assembly

Objective To analyze the surgical treatment options and its results for thoracic and thoracolumbar disc herniation.Methods From June 2004 to December 2009,thirty-one patients of thoracic and thoracolumbar disc herniation with or without ossification of ligament flavum were surgically treated,including 22 males and 9 females,with a mean age of 54 years(range,24 to 71 years).According to Anand and Regan clinical classification,there was type 2 for 1 case,grade 3a for 2,grade 3b for 3,grade 4 for 6,and grade 5 for 19.The neurological status was Frankel B for 2 cases,C for 6,D for 11,and E for 12.Anterior surgeries were performed for 18 patients without ossification of ligament flavum.Anterior decompression was performed through the resection of posterior part of vertebral body,or subtotal resection of vertebral body,followed by strut graft and internal fixation.Posterior surgeries were performed for 13 patients with disc herniation and ossification of ligament flavum.The resection of hemi-articular process and total laminectomy was performed.Results The complications of 18 patients with anterior surgery included laceration of dura mater in 1 case,nerve root sleeve injury in 1 case,intercostal neuralgia in 3 cases,atelectasis in 1 case,and femoroiliac numbness in 2 cases.The complications of 13 patients with posterior surgery included intra-canal hematoma in 1 cases,leakage of cerebrospinal fluid in 2 cases,infection of incision in 1 cases,and pneumonia in 1 case.The patients were followed for 18 months(range,6 to 48 months).At final follow-up,the neurological status and local symptom improved in all patients,with Frankel C for 3 cases,D for 7,and E for 21;and Anand and Regan type 1 for 2 cases,2 for 1,3a for 1,4 for 2,5 for 10 and no symptom for 15.Conclusion For thoracic and thoracolumbar disc herniation,anterior surgery is suitable for patients mainly suffered anterior cord compression.Resection of posterior part of vertebral body or subtotal resection of vertebral body is often needed for sufficient decompression.Posterior surgery is suitable for patients with anterior and posterior cord compression due to ossification of ligament flavum,and the decompression can be obtained by resection of hemi-articular process and total laminectomy.

OBJECTIVE: To realize the application of nasal obstruction symptom evaluation (NOSE) and Epworth sleep score (ESS) before and after nasal cavity ventilation expansion techniques. METHOD: Forty-two OSAHS patients with nasal obstructive symptoms were diagnosed by clinical symptoms and polysomnography. Nasal cavity ventilation expansion technique was performed. Before and after the surgery, the NOSE and ESS were used to compare the difference. Postoperative data were obtained at least 3 months later. RESULT: Compared the data before and after operation, the NOSE and ESS were significantly decreased respectively (P<0. 05), there is no significant difference between 3 groups (P>0. 05). CONCLUSION The result suggest that OSAHS patients taking nasal cavity ventilation expansion operations showed improvement in severity of nasal obstructive symptoms and daily sleepy.
Humans ; Nasal Cavity ; surgery ; Nasal Obstruction ; physiopathology ; surgery ; Nasal Surgical Procedures ; Paranasal Sinuses ; Polysomnography ; Sleep ; Sleep Apnea, Obstructive ; physiopathology ; surgery ; Surveys and Questionnaires ; Symptom Assessment ; methods

Objective To analyze the mutations of IDS gene in a mucopolysaccharidosis type Ⅱ (MPS Ⅱ) family and to make prenatal diagnosis on the high-risk fetus which has been pregnant for eleven weeks.Methods IDS gene was analyzed by bidirectional DNA sequencing in 2 patients and their mother,and 5 unaffected individuals.Prenatal diagnosis for the high-risk fetus was performed by chorionic villus sampling after the genotypes was identified.Results The mutation c.344delA (N115fsX15) was detected in the two patients,and the mother of patients carried the heterozygous c.344delA (N115fsX15) mutation.None of the mutant was detected in the 5 unaffected subjects.The fetus carried c.344delA (N115fsX15) heterozygous mutation and was a carrier.Conclusion The deletion mutation c.344delA (N115fsX15) is causative to the pedigree of MPS Ⅱ,and prenatal diagnosis is the efficient method to avoid defect birth.

OBJECTIVE To understand the pathogenicity of Staphylococcus epidermidis causing urinary tract infection. METHODS Thirty five isolates were collected in the patients with urinary tract infection during 2003-2004 to test the minimal inhibitory concentration(MIC) to erythromycin,ampicillin,cefoxitin,ceftriaxone,teicoplanin,ciprofloxacin,tetracycline,trimethoprim-sulfamethoxazole and vancomycin.The genes of ermA,ermB,ermC,msrA,mecA,and icaA were amplified by PCR.The biofilm producing of the isolates was determined by the quantitative method.The pulsed-field gel electrophoresis(PFGE) was used to discriminate the homology among the isolates. RESULTS Among the isolates,MRSE accounted for 71.4%.The antibiotic susceptibility to erythromycin,ampicillin,ceftriaxone,ciprofloxacin,tetracycline and trimethoprim-sulfamethoxazole were 22.8%,6.34%,34.9%,26.9%,44.4% and 62.2%,respectively,whereas to teicoplanin and vancomycin were 99.2% and 100%.All the isolates resistant to cefoxitin carried mecA.ermC Was the predominant determinant among the erythromycin resistant isolates.Among the 14 icaA positive isolates,10 isolates were biofilm producing.All the isolates were classified into 18 types by PFGE. CONCLUSIONS The high and multiple antibiotic resistance of S.epidermidis isolates causing urinary tract infection were shown.The detection of icaA gene in clinical S.epidermidis isolates was a signal of their pathogenicity.

Aim To study the effect of polysaccharides from Gastrodia elata Blume(GEP) on levels of superoxide dismutase(SOD),malondialdehyde(MDA) and nitric oxide(NO);and the retinal morphology after rat retina ischemia reperfusion injury.Method Retinal ischemia was induced in SD rats by increasing the intraocular pressure to 16 kPa for 60 min via cannulation into the anterior chamber.SD rats were divided into five groups randomly: Small dose GEP group,Middle dose GEP group,Large dose GEP group,Vitamin E group and Negative contral group.At 60 min,24 h and 72 h after ischemia reperfusion,levels of SOD,MDA and NO in rat retinas were measured,and histopathologic changes were observed.Results At 60 min,24 h and 72 h after ischemia reperfusion,levels of SOD in rat retinas in middle,large dose GEP groups were significantly higher than that in negative control group(P

Objective To study the effect of powder of Chinese Angelica and Peony (DSS) on hippocampus proteome of senescence accelerated mice (SAM). Methods Thirty female mice aged 12 months were used in our study, including 10 SAM-resistance/1 (SAMR1) and 20 SAM-prone/8 (SAMP8). The difference of hippocampus proteome among SAMP8, SAMR1, SAMP8 treated with DSS intragastrically at dose of 3.2 g/kg once a day for 30 d (SAMP8+DSS), was analyzed by two dimensional polyacryalmide gel electrophoresis (2DE). Results Compared with SAMR1, the expressions of 13 proteins in hippocampus of SAMP8 were up-regulated and that of 9 proteins were down-regulated significantly. Compared with SAMP8, the expressions of 21 proteins in hippocampus of SAMP8+DSS were up-regulated and that of 14 proteins were down-regulated significantly. Using MALDI-TOF-MS, the proteins with significant changes in expressions were identified by peptide fingerprinting map and the results were searched in MASCOT database, and sorted into 5 function groups including mitochondria-associated proteins, energy metabolism proteins, synaptic plasticity formation proteins, cytoskeletal proteins and others proteins. Conclusion The effects of DSS on hippocampus proteome of senescence accelerated mice were probably exerted via multi-target and multi-pathway mechanism.