Objective To investicate the effect of apoptosis of human ovarian cancer cell line SKOV_(3) and 3AO exposed to arsenic trioxide on telomerase activity and its mechanisms.Methods The human ovarian cancer cell line SKOV3 and 3OA were treated with arsenic trioxide of different concentration for 12,24,72 h.Cell morphology,PCR-ELISA,RT-PCR were adopted to detect the cell apoptosis and telomerase activity and the expression of human telomerase catalytic subunit(hTERT).Results Arsenic trixide could induce apoptisis of ovarian cancer cell lines,but there exists difference in drug concentration,type of cell line.A dose and time-dependent decline of telomerase activity after SKOV_(3) and 3AO cells exposed to arsenic trixide,meanwhile hTERT mRNA was down-regulated.Conclusion Telomerase activity and hTERT mRNA expression have close relationship and play an important role in arsenic trioxide inducing SKOV_(3) and 3AO cells apoptosis.

Objective To investigate the morphological changes of mitochondria and the expression of bcl-2 in the apoptotic SKOV3 and 3AO cells induced by arsenic trioxide (As2O3). Methods Light and electron microscopy, flow cytometry analysis, immunofluorescence flow cytometry analysis were used to detect the apoptotic cells, ultrastructural alteration of mitochondria, and the changes of mitochondrial transmembrane potentials (??m). Results As2O3 induced apoptosis of ovarian cancer cell lines was in a dose dependent manner and various in different cell lines. As2O3 also made a decrease of ??m in SKOV3 and 3AO cells in a dose independent fashion. Electron microscopy indicated that the mitochondria showed swollen, balloon-like appearance and outer membrane disrupted 72 h after As2O3 treatment. Expression of bcl-2 was down-regulated in SKOV3 and 3AO cells after As2O3 treatment. Conclusion The reduce of ??m and down-regulation of bcl-2 may play the key roles in the process of As2O3-induced apoptosis.

Objective To comparetheproximalfemoralnailantirotation(PFNA)anddynamichipscrew(DHS)treatmentonin-tertrochanteric femur fractures in the elderly .Methods Retrospective analysis was conducted on 60 patients with intertrochanteric femur fracture collected from December 2009 to December 2012 ,which underwent these two methods ,including 32 cases of PFNA group ,28 cases of DHS group .The 5 indicators including operation time ,intraoperative blood loss ,complications ,fracture healing time and hip function recovery were analyzed .Results Sixty cases were followed up for 10 to 25 months ,average 17 months ,com-pared with DHS group ,PFNA group was with short operation time ,less bleeding ,less complications ,short time of fracture healing , according to standard of Harris score ,the hip function recovered well .Conclusion PFNA treatment on intertrochanteric femur frac-tures is with little trauma ,reliable fixation ,fewer complications ,with which the hip function recovered well ,and it is the preferred method of operation in treatment of senile intertrochanteric femur fracture .

It has been demonstrated that surgery is currently still the most effective approach to completely cure gastric cancer. However, while only 30%-40% of gastric cancer can obtain curative outcome through pure surgery, most of patients with gastric cancer would died of recurrence or distant metastasis of the tumor. It appears to be more critical to search for other therapeutic strategies for gastric cancer other than surgery. Molecular targeting therapy has become the focus and hotspot of comprehensive treatment of gastric cancer.

ObjectiveTo investigate the effects of cervical stability training on cervical vertigo. Methods67 patients were divided into 2 groups. The experiment group was treated with Neurac (neuromuscular activation) for cervical stability training, meanwhile, the control ones with manipulation. They were assessed with clinical assessment and fall index before and 14 d after treatment. ResultsThe scores of clinical assessment and value of fall index were significant improved more in experiment group than in controls （P＜0.05）. ConclusionSpecific cervical stability training is effective on cervical vertigo based on cervical instability and proprioceptive disorder.

Objective:To investigate the effects of bone morphogenetic protein 2(BMP-2)expression in the related periodontal tissue on bone remodeling under different distracting force during rapid tooth movement by resistance reduction and distraction. Methods:1 2 Beagle dogs were randomly divided into 4 groups as follows:5 d distraction,1 5 d distraction,1 5 d distraction and 1 0 d retaining and 1 5 d distraction and 90 d retaining.4 4 were distalized.6 teeth in each group were randomly assigned to re-sistance and distracting method,resistance and conventional method and conventional method,and there were 2 teeth in each group.Moving teeth models were prepared regularly.BMP-2 expression was examined by immunohistochemistry.Results:The BMP-2 positive expression of the 3 groups of different distraction schedule showed similar distribution area,and it reached peak at the end of 1 5-day distration,but the group of resistance and distracting method showed the maximum peak(P <0.05)and maximum tooth movement(P <0.01 ).In different stages of distraction,the positive expression in the group of resistance reduc-tion and conventional method was higher than in the group of conventional method but lower than the group of resistance reduction and extraction method(P <0.05).There was no significant difference in BMP-2 positive expression among 3 groups when retai-ning was 90 d(P >0.05).Conclusion:Resistance reduction with sustained strong distracting force can significantly increased the positive expression of BMP-2 and effectively accelerate new bone formation in periodontal tissue.

Objective To investigate the correlation factors of neonatal lenticulostriate vasculopathy. Method Four hundred and forty-seven newborns from Guangdong Women and Children Hospital were enrolled in this study. Clinical data of the newborns were obtained . Brain ultrasound studies of lenticulostriate artery were performed on the newborns. The logistic regression was performed for screening the correlation factors of neonatal lenticulostriate vasculopathy (P < 0.05). Results Results of the univariate logistic regression reveal the correlation factors tcontributing to LSV include congenital cytomegalovirus infection、neonatal asphyxia、congenital heart disease (CHD),hypertensive disorder in pregnancy (P < 0.05, respectively). Multivariate logistic regression analysis was performed on these factors. The congenital cytomegalovirus infection, neonatal asphyxia, CHD,hypertensive disorder in pregnancy were significantly associated with LSV (P < 0.05). Conclusion The congenital cytomegalovirus infection,neonatal asphyxia,CHD,hypertensive disorder in pregnancy are the correlation factors of neonatal lenticulostriate vasculopathy. LSV could be a predictive marker for the future development of neuropsychiatric disorders. The brain ultrasound studies of lenticulostriate artery is suggested to be performed on all infants with the correlation factors.

Objective To study the relationship between homocysteine (Hcy)and carotid atherosclerosis in patients with cer-ebral infarction.Methods During January and November 2013,281 patients with cerebral infarction from Affiliated Hospital of Yan’an University were provided the blood samples as cerebral infarction group,140 healthy volunteers served as control group.Serum Hcy was detected by enzymatic cycle assay.According to the results of carotid artery ultrasonography,cerebral infarction group was divided into five groups:artery normal group,intimal thickening group,soft plaque group,mixed plaque group and hard plaque group.The statistically significance was analyzed by SPSS 19.0.Results The serum Hcy level of cer-ebral infarction group was 19.78 ± 5.21 μmol/L,significantly higher than the control group 10.24 ± 3.33 μmol/L (P <0.001).The serum Hcy levels of control group,artery normal group,intimal thickening group,soft plaque group,mixed plaque group and hard plaque group were 10.24±3.33,15.20±2.99,17.03±1.85,25.44±4.24,19.65±4.74 and 18.31 ±3.67 μmol/L respectively.The differences between groups were statistically significant in addition to intimal thickening group and hard plaque group (P =1.106).The positive rates were 16.4%,53.7%,87.1%,95.7%,83.1% and 77.3% re-spectively,the groups which in cerebral infarction group compared with control group,the differences were statistically sig-nificant (P <0.001).Conclusion Hcy played an important role in the occurrence and development of cerebral infarction. Lower serum Hcy concentration,may be an effective way to prevent carotid atherosclerosis and cerebral infarction.

Objectives To study the status of medical compliance and the reason of non-medical compliance behavior of the Hui diabetics. Methods 121 Hui diabetics were recruited by convenient sampling, investigated by medical compliance questionnaire and interviewed by open questions about the reason of non-medical compliance behavior (e.g. drug therapy, diet therapy, sports therapy, self monitoring and periodic review). Results The mean score of the medical compliance behaviors was (32.69±8.64), and the overall rate of non-medical compliance was 95.9%(116/121) in Hui diabetics. Arranged by the rate of non-medical compliance in descending order, the five dimensions of medical compliance behaviors were sports therapy, self monitoring, periodic review, diet therapy and drug therapy in turn. The reason of non-medical compliance behavior mainly included the cognition of treatment, the economic conditions, job, minority customs and religion. Conclusions Non-medical compliance behaviors existed in five major diabetes therapies in the Hui diabetics. The patients and their families should be educated and conducted individually according to their specific situation to improve the medical compliance behavior.

Objective:To visually analyze the general situation and development of pre-oxygenation research abroad, and provide a reference for further development of pre-oxygenation in China.Methods:Using the WOS core data set as the data source, CiteSpace V software was used to visually analyze the literature published from January 1, 2000 to November 29, 2019.Results:The number of papers has shown a rapid upward trend since 2013, the US has the most papers. Research hotspots mainly focus on tracheal intubation, intensive care unit, apnea oxidation, non-invasive ventilation and complications.Conclusion:The United States is in the leading position in the study of pre-oxygenation, and China is still in the initial stage in the study of pre-oxygenation. We should strengthen cooperation and exchange with developed countries such as Europe and America. The application of pre-oxygenation outside the operating room and the selection of pre oxygenation tools are the direction of further research.