Objective:To investigate the effects on the activity of acetylcholinesterase (AchE) and choline acetylase(ChAT) in filial rat’s brian and learning and memory capacity after the mother-rat is exposed to nonylphenol. Methods:At the first day of the pregnancy,the rats were divided into four groups,which were orally administered with NP at doses of 0,50,100 and 200 mg/kg,respectively,on gestational days 9～15. The offspring rats’s Brain tissues of each groups were dissected for AchE and ChAT activity assay and cognitive function was tested by Morris water maze. Result:Morris water maze test results showed the escape latency was longer in the groups of NP 200 mg/kg than in control group(P

Objectives To investigate the effect of carbon dioxide pneumoperitoneum on postoperative cognitive dysfunction and the level of serum NSE and S-100β protein in female patients undergoing gynecological laparoscopy. Methods 60 ASA physical status Ⅰ patients were divided two groups, group Ⅰ received no insufflation andconventional abdominal surgery ( n = 30) and group Ⅱ received abdominal insufflation and gynecological laparoscopy ( n =30). MMSE was recorded at several different time points, including one day before operation, 1, 6, 24, 48, 72h after operation, and before discharge. Serum S-100β protein and NSE was measured by ELISA before the beginning of operation ( or carbon dioxide pneumoperitoneum) and 1h after operation (or carbon dioxide pneumoperitoneum). Results MMSE values at 1,6,24,48,72h decreased significantly in group Ⅱ (24. 67 ± 1.47,25.97 ± 1.50,26. 77 ± 1.61,27.07 ± 1.87,27.37 ± 2. 06) after operation, compared with group Ⅰ (27.63 ± 1. 33,27.27 ± 0. 87,28.37 ± 0. 85,28.73 ±0. 78,29. 23 ±0. 86, P <0. 01 ). And the baseline value (29. 17 ±0. 76) of serum S-100β[(0. 114 ±0. 012,0. 086 ±0. 009) μg/L] protein and NSE [( 13. 720 ± 1. 330,12. 093 ±0. 697) μg/L] increased significantly at 1h after operation in group Ⅰ and Ⅱ compared with before operation [(0. 035 ±0. 030,0. 035 ±0.024;5.753±0.889,5.831 ±0.967)μg/L, P <0.01]. Serum S-100 β protein[(0. 114 ±0.012) μg/L] increased significantly at 1h after operation in group Ⅱ, compared with group Ⅰ [(0. 086 ±0. 009) μg/L,P < 0. 05], whereas NSE showed not difference [( 12. 093 ± 0. 697,13. 720 ± 1. 330) μg/L, P > 0. 05].Serum of S-100β protein and MMSE were significantly correlated w group Ⅰ and Ⅱ ( r = 0. 6412,0. 8126, P <0.01). Serum NSE was not correlated with the MMSE score in group Ⅰ ( r =0.4397, P >0.05),whereas NSE and MMSE had significant correlation in group Ⅱ ( r = 0. 7111, P <0. 01 ). Conclusions Carbon dioxide pneumoperitoneum in patients with gynecological surgery might affect postoperative cognitive function, and MMSE score was negatively correlated with serum S-100β and NSE proteins.

Objective To investigate the effect of a modified cuff insertion technique named “portal vein branch-sling” (PBS) method for portal vein (PV) anastomoses in rat liver transplantation.MethodsA total of 60 cases of orthotopic liver transplantation (OLT) using 2-cuff technique in rats were performed in our transplantation center.The PBS technique and conventional methods were used respectively in study and control groups.Time for PV anastomoses,anhepatic phase (AP) and surgery of recipients,successful rate for PV cuff insertion and operation were statistically compared between the different groups.Results35 cases of OLT using PBS technique in rats were assessed.The successful rate of PV cuff insertion was 94.3％,and the time of PV anastomoses using the modified PBS cuff insertion technique was only 1.9 ± 0.7min.The successful rate of operation was 80％with a 21.8±2.2min AP.The study group showed significant differences (P＜0.05) compared with conventional group.The survival rate in the first week was 85.8％ with no statistical difference (P＞0.05).ConclusionsThe modified PBS cuff insertion technique for PV anastomoses of OLT has advantages in speed,safety and stabilization,and should be considered as one of the best available PV anastomosis technique for OLT in rats.

Objective To investigate the protective effect and mechanism of naloxone preconditioning ( NP) for rats with cerebral hemorrhage. Methods Totally 30 healthy male Sprague Dawley ( SD) rats were randomly separated into Sham operatopm group,intracerebral hemorrhage ( ICH) and NP treatment groups ( n =10) . Before the establishment of rat cerebral hemorrhage model,rats in the NP group were given an intraperitoneal injection of naloxone hydrochloride ( 2. 0 mg/kg) ,and those of ICH group received an injection of normal saline at same volume. Then,the ICH model was made by injecting 0. 4 IU collagen Ⅶ into caudate nucleus while. The brain tissue samples around cerebral hemorrhagic focus after ICH were collected. RT-PCR and Western blotting were used to detect the transcriptional level and protein level of aquaporin protein 4 ( AQP4) in the brain tissue,respectively. Meanwhile,immunohistochemistry was employed to determine the expression of matrix metalloproteinase 9 ( MMP-9) in the rat brain tissue. Results Brain water content in ICH group was larger than that in Sham group ( P

Related studies have been confirmed that tumor-associated inflammation is associated with tumor progression and prognosis in a variety of tumors.As an inflammatory biomarker,lymphocyte-monocyte ratio shows significant value in predicting the prognosis of malignant tumors.It can not only be appropriate for patients with cancer risk stratification,but also provide the basis for the subsequent development of individua-lized treatment programs.

Objective To analyze the clinical symptoms and CT findings of fungus sinusitis in order to evaluate CT diagnosis. Methods To analyze retrospectively the collected data of 12 patients who underwent operations for fungos sinusitis which was proved pathologically. Results The main clinical feature of fungos sinusitis was bloody supperative rhinorrhea in all cases. In the CT scans, we found the density of the involved sinus cavities rised inhomogeneities in all patients. CT scans also showed spot-like calcification in 7 cases, bony proliferation in 5 cases and bony absorption in 2 cases. The operation revealed a lot of filthy or inflammatory polypoid substances in the involved sinus cavities in all patients. Pathological examination demonstrated clusters of fungus in the filthy substances. Conclusion The diagnosis of fungus sinusitis mainly derived from clinical symptoms and radiological examinations. The final diagnosis depended on pathology. It is of great value for reference that CT scans can show the anatomical structures of the sinuses and help to diagnose fungus sinusitis.

BACKGROUND:Unicompartment knee replacement is more popular for smal trauma, rapid recovery, low complication and almost normal knee mechanics, and has been more and more used in clinic to repair single compartment knee disease. At present, there is no report about the influence of the angle of the single condyle prosthesis on the survival rate. OBJECTIVE:To evaluate the effectiveness of unicompartmental knee replacement for medial compartmental osteoarthritis of the knee in 4-6 years of fol ow-up. METHODS:Thirty patients with medial compartmental osteoarthritis of the knee were treated by unicompartmental knee replacement with Oxford system. Complication occurrence was observed. Curative effects were evaluated with Hospital for Special Surgery score before and 3 months, 6 months and 1 year after surgery. On anteroposterior view, the varus/valgus alignments of the tibial components were measured relative to the long axis of the tibia. On lateral view, flexion/extension of the femoral component was measured relative to the posterior femoral cortex. RESULTS AND CONCLUSION:(1) Primary healing of incision was obtained in al patients, and no infection or lower limb deep venous thrombosis occurred. (2) Al of the patients were fol owed up for 48-72 months. There was no prosthetic loosening, dislocation or revision for contralateral compartment and patel ofemoral joint symptoms. (3) Hospital for Special Surgery score was significantly increased to (90.47±4.05) (P=0.00). (4) Tibial placement angle of single condyle prosthesis:The axis of the prosthesis was perpendicular to the axis of the tibia in 21 cases. Varus placement:2° in 1 case, 4° in 3 cases, 5° in 2 cases, 6° in 2 cases, and 10° in 1 case. No valgus occurred in the tibial side. (5) Femoral prosthesis placement angle:The axis of the femoral prosthesis was paral el to the posterior edge of the femoral cortex in 22 cases, on the flexed position:4° in 2 cases, 5° in 1 case, 6° in 1 case and 7° in 1 case;in the extension position:3° in 1 case, 4° in 1 case and 5° in 1 case. (6) Results suggested that the unilateral condylar replacement for the repair of medial compartment osteoarthritis of the knee has a good initial effect. The middle-term and long-term efficacy needs longer fol ow-up study.

Patients with malignant tumor are easy to be complicated with venous thrombosis,which is the second leading cause of death for patients with malignant tumor.The mechanism of tumor thrombus forma-tion is related to tumor type,treatment,metastasis,heredity and tumor itself.Evaluating the risk of venous thrombosis in patients with cancer and providing targeted preventive anticoagulation measures for high-risk patients have important significances for improving the quality of life and prolonging the survival period of pa-tients with cancer.

Objective To study the effect of early rehabilitation on motor function of hemiplegic patients after cerebral infarction. Methods Forty two patients were divided into two groups randomly: a rehabilitation group and a control group. Both groups were given the same internal medical treatment to stabilize the patients conditions in the early stage. In addition, the patients in the rehabilitation group were given early formal rehabilitation training, while those in the control group were given no formal rehabilitation training. The results of therapy were assessed by using SIAS method (Stroke Impairment Assessment Set). Results As shown by the SIAS result, the scores of motor function, muscle tone, range of motion (ROM), pain, trunk control and function on the unaffected side in the rehabilitation group were significantly better than those in the control group (all P

Studies have shown that cholesterol metabolism plays an important role in tumorigenesis and development.At the same time, it is also shown an important application value in predicting the prognosis of malignant tumors.Cholesterol can improve the long-term quality of life and survival of cancer patients by appropriating risk stratification of these malignant tumors.