Diffuse large B-cell lymphoma (DLBCL) is the aggressive non-Hodgkin's lymphoma.Rituximab in combination with chemotherapy had improved the outcomes,however,a substantial proportion of these patients transfer into refractory or eventually relapsed lymphoma after using R-CHOP as a front-line treatment regimen with poor prognosis.With the research development on DLBCL and its related gene,it is confirmed that gene biological targeted therapy can improve the prognosis of patients with refractory /relapsed DLBCL (RR-DLBCL).Recently,some new targeted therapies become research hotspots.This review will focus on recent development and future direction of biological targeted treatment in RR-DLBCL.

Acute kidney injury (AKI) is the most common and serious complication in the intensive care unit.The mortality of AKI is still as high as 60％ in critically ill children because of lack sensitive and special early predictive biomarker.Neutrophil gelatinase-associated lipocalin is one of the most promising early diagnose and predict biomarkers for AKI,produced and secreted by kidney tubule cells at low levels,but increasing dramatically in the urine and serum after ischemic, sepsis, or nephrotoxic injury of the kidneys.This review is to summarize the role of neutrophil gelatinase-associated lipocalin as an early biomarker of AKI with different clinical settings in children.

Objective To investigate the effects of different flow volume of continuous 1:1 blood crystalloid cardioplegia on open heart surgery.Methods Retrospective randomized study was performed on 80 patients undergoing valve replacement and congenital heart disease with 1:1 blood crystalloid cardioplegia by minimum-flow continuous perfusion[0.5ml/(kg·min),group A,40 cases],high-flow continuous perfusion[(2.0 ml/(kg·min),group B,40 cases).The changes of creatine kinase(CK),isoenzyme of creatine kinase(CK-MB),lactate dehydrogenase(LDH),lactic dehydrogenase isoenzyme(LDH_1)were detected before operation and at 6,12,24,48,72 h after operation.Results The cardioplegia liquid was (41.72±7.98)ml/kg in group A,(142.09±9.65)ml/kg in group B,there was significant difference between two groups(P<0.05).During the first 72 h postoperative the value of CK,CK-MB,LDH and LDH1 in two groups were increased to different degree,serum enzymes levd increased significantly between 12,24 h after operation(P<0.05)and return to normal at 72h after operation in two groups.Butbetween two groups there was no significant difference(P＞0.05).ConclusionMinimum-flow continuous perfusion of 1:1 blood crystalloid cardioplegia appears to be superior in safety,decrease of myocardial enzymes integrity.

Objective To explore the effect of cognitive therapy on patients with traumatic paraplegia.Methods Toally 100 traumatic paraplegia patients were randomly divided into observation group and control group with 50 cases in each.The control group received routine nursing intervention and the observation group cognitive therapy on the basis of intervention for the control group.The two groups were compared in terms of anxiety,depression,mental health,suicidal ideation and quality of life by the instruments of self-rating anxiety scale (SAS),self-rating depression scale (SDS) and suicide attitude questionnaire (SQA) before and after intervention.Results Before the intervention,the scores by SDS,SAS scores and SQA were insignificant different between the groups (P＞0.05).Mter the intervention,the scores on suicidal ideation in the observation group were significantly higher and the quality of life were significantly higher and the depresion and anxiety and the mental health status were lower than those of the control group (P ＜0.05).Conclusion The cognitive therapy for the patients with traumatic paraplegia can significantly alleviate their depression and anxiety,reduce their suicidal ideation and improve the quality of life.

The major target of translational medicine is the rapid application of achievements in basic research to clinical medicine. How to carry out embedded subject service for translational medicine in academic library was thus described in aspects of constructing its information service platform, establishing its service team, and deciding its service methods.

Hypoglycemia is a common metabolic problem in newborn infants,which might lead to brain injury even brain death when it keeps extremely low for a long time.Therefore,it is of great importance to monitor blood glucose in neonates,especially in groups of infants who are at risk of hypoglycemia,in order to identify and treat this condition and prevent adverse neurological outcomes promptly.It is given that infant of a diabetic mother,preterm neonate,low birth weight and asphyxia are its risk factors.Relative to blood glucose monitoring methods in clinical,continuous glucose monitoring system is comprehensive,continuous and with low pain.Continuous glucose monitoring system has been applied in high-risk neonates of hypoglycemia in recent years.It is of great help to understand the complete picture and change trend of blood glucose,optimize blood glucose management,study newborn glucose metabolic characteristics and the relationship between hypoglycemia and brain damage,so as to define and manage neonatal hypoglycemia.

Objective To explore CT findings of pulmonary non‐tuberculosis mycobacteria (NTM ) disease .Methods Forty‐two patients with pulmonary NTM disease confirmed by the biphasic medium flora identification (NTM group) ,and 60 patients with lung tuberculosis (TB group) confirmed by the tubercle bacillus cultivation and flora identification in our hospital were included in the ret‐rospective analysis .9 CT signs and distribution features of the lesions were analyzed and compared between the two groups .The difference was statistically significant if P<0 .05 .Results Pulmonary NTM disease was more common in female patients (χ2=5 .500 ,P=0 .019) ,and the mean age was significantly older than that of the tuberculosis (t=3 .456 ,P=0 .001) .The detection rate in the right middle lobe and left tongue section was high in NTM group than in TB group (χ2 =8 .361 ,P=0 .004) .Logistic regression showed that bronchiectasis and bronchial stenosis or occlusion were independent risk factors for the NTM disease .They were important signs for the differential diagnosis from tuberculosis .Conclusion MSCT findings of pulmonary NTM disease have certain characteristic , which are helpful for the diagnosis .

Atherosclerosis is the pathological basis of ischemic stroke. Peripheral blood leukocytes are involved in the occurrence and progression of atherosclerosis and cause vascular endothelial injury. A series of changes of peripheral immune cel take place after acute ischemic stroke and cause inflammatory cel infiltrating into the brain tissue. The elevated leukocyte count is closely associated with the occurrence and prognosis of coronary atherosclerotic heart disease. Studies have also shown that the elevated leukocyte count may influence the occurrence of ischemic stroke, neurological deficit, recurrence, and death. This article reviews the relationship between peripheral blood leukocytes and ischemic stroke.

Objective:To study the microwave-assisted extracting method for magnolol and honokiol in Magnolia offcinalis Rend et Wils.Methods:The orthogonal design was used to study the extraction method of magnolol and honokiol.The contents of them were determined by HPLC.The methanol water glacial acetic acid(85:15:0.25)was used as a mobile phase.The flow rate was 1.0ml/min,the detection wavelength was 294nm and the column temperature was 23℃.Results:The condition of 75%ethanol with the middling power of microwave,extracting for 4 minutes and ratio of materials to liquid 1:40 was the optimal extracting method.The extracting rate of honokiol and magnolol was 92.33%,73.59%.The RP-HPLC for determining method was simple,rapid and accurate.Conclusion:The extracting and determining method was practicable for magnolol and honokiol in Magnolia offcinalis Rend et Wils.

Objective To investigate the protective effect and mechanism of naloxone preconditioning ( NP) for rats with cerebral hemorrhage. Methods Totally 30 healthy male Sprague Dawley ( SD) rats were randomly separated into Sham operatopm group,intracerebral hemorrhage ( ICH) and NP treatment groups ( n =10) . Before the establishment of rat cerebral hemorrhage model,rats in the NP group were given an intraperitoneal injection of naloxone hydrochloride ( 2. 0 mg/kg) ,and those of ICH group received an injection of normal saline at same volume. Then,the ICH model was made by injecting 0. 4 IU collagen Ⅶ into caudate nucleus while. The brain tissue samples around cerebral hemorrhagic focus after ICH were collected. RT-PCR and Western blotting were used to detect the transcriptional level and protein level of aquaporin protein 4 ( AQP4) in the brain tissue,respectively. Meanwhile,immunohistochemistry was employed to determine the expression of matrix metalloproteinase 9 ( MMP-9) in the rat brain tissue. Results Brain water content in ICH group was larger than that in Sham group ( P