Rabbit anti-TSH anti-idiotypic antiscra were raised by immunization with auti-TSH antibody. The antiserum from rabbit No. 551 was found to have the activity of inhibiting the binding of TSH receptor on thyroid cell membrane and 125I-TSH, that is, TBII (TSH-binding inhibitor immunoglobulin) positive. This antiserum was also shown to inhibit cAMP release stimulated by 100 mU/L bTSH. The characteristics of No. 551 antiserum were similar to of patients with myxedema.

In this work, a simple and sensitive assay for soluble IL-2 receptor (sIL-2R) was established. The main procedures are as follows: the microplate was coated with anti-Tac monoclonal antibody (McAb); after sIL-2R in samples bound with the McAb, the rabbit anti-IL-2R serum was added;and HRP-goat anti-rabbit IgG was used to display and amplify this binding. The sensitivity of this method is about 100u/ml, which is near that in "Cellfree IL-2 Receptor Test Kit".

Serum soluble IL- 2 receptor (sIL-2R) level of 34 patients with leukemia were measured by ELISA. The sIL- 2 R levels are 296?99u /ml in normal, 660 ?449u /ml in AML, 1323 ?755 u/m in ALL, 1577 ? 759 u /ml in AMML and 1815 ? 858 u /ml in NHL. 5 of 6 patients with 2000u /ml sIL- 2 R died in one month after detection of sIL-2R. There is no apparent relationship between sII -2 R level and mononuclear cells count in peripheral blood. The results suggest that it is possible to be a new parameter for the observation and prognosis estimation to patients with leukemia by detection of sIL-2R.

The cancer stem cell theory suggests that cancer develops from a subset of tumor cells that possess characteristics of stem cells. Breast cancer stem cells comprise a sub-population, which possesses the capacity of self-renewal and the potential for differentiation and high tumorigenicity. Evidence from bothin vitro andin vivo studies demonstrates breast cancer stem cells are responsible for tumor relapse, invasion and metastasis, chemo- and radio-resistance and epithelial-mesenchymal transition (EMT). Herein, this review highlighted the recent advances in breast cancer stem cells.

Objective To analyze the curative effect of double path endoscopic surgery in the treatment of fungal sinusitis,and to compare with the effect of simple in endoscopic nasal passages windowing.Methods The clinical data of fungal sinusitis patients were analyzed.According to the different surgery way,the patients were divid-ed into two groups.The observation group:nasal endoscopic nasal passages open a window in the joint of fangs nest small windows double path;Control group:simple in nasal endoscopic fenestration.Results The observation group had 35 cases and the control group had 37 cases.After treatment,the symptoms score of the observation group was sig-nificantly lower than the control group [(1 .09 ±0.83)points vs (1 .47 ±0.77)points;t =2.01 5,P =0.048].The effective rate of the observation group was significantly higher than the control group (94.29% vs 75.68%;χ2 =4.81 3,P =0.028).7 days after treatment,the C -reactive protein(t =4.971 ,P <0.01 ),TNF alpha(t =9.1 9,P <0.001 ),beta IL -1 (t =5.635,P <0.01 ),white blood cells (t =3.25,P =0.002)and IL -6(t =3.86,P =0.002) in the observation group were significantly lower than the control group (P <0.05).The preoperative plasma level of 1 -3 -β-glucan D between the two groups had no statistical difference (P >0.05).2(t =5.978,P <0.01 ),4(t =6.335,P <0.01 ),6(t =9.348,P <0.01 ),and 8 days(t =8.226,P <0.01 )postoperatively,1 -3 -β-glucan D levels were significantly lower than the control group,the differences were statistically significant (P <0.05).The postoperative recurrence rate of the observation group was significantly lower than the control group (8.57% vs 27.03%,the Log -rank χ2 =4.061 ,P =0.044).Conclusion In fungal sinusitis treatment,the use of endoscopic nasal passages open window joint fangs nest small windows double path can more effectively remove lesions,its forward curative effect is superior to pure in nasal endoscopic fenestration.

Nanotechnology has been extensively applied in the diagnosis and treatment of cancer, and is currently a major focus of research worldwide.Among the various nanomaterials, gold nanoparticles (GNPs) are of particular interest to many researchers due to their superior properties of low cytotoxicity, good biocompatibility, and preferential accumulation in tumors (the enhanced permeability and retention effect), which in turn gives GNPs a potential application in cancer diagnostics, imaging, photothermal therapy, and radiotherapy.Many published studies have shown that GNPs can be used as a new ideal radiosensitizer, and therefore understanding the radiosensitizing effects of GNPs both in vitro and in vivo will be of great significance in the clinical translation of nanomedicine.

Objective To analyze the factors related to glare occurred after the phakic intraocular lens (P-IOL) implantation,and to raise the standard for the P-IOL selection.Design Retrospective case series.Participants 104 patients with high myopia underwent P-IOL implantation in Beijing Tongren Hospital.Methods In 104 cases,22 cases (22 eyes) were implanted angle supported Phakic6 designed accord with corneal symmetry,54 cases (54 eyes) were implanted iris fixed Verisyse,and 28 cases (28 eyes) were implanted posterior chamber PRL.Selection of the diameter of P-IOL optics depended on P-IOL diopters.The pupil diameter,the P-IOL optic center position,the pupil and corneal center position were measured with anterior segment optical coherence tomography (OCT).The contrast sensitivity (CS) under different condition (day,day and glare,night,night and glare) and in different space frequency (1.5,3,6, 12,18c/d)were examined with Optec6500 system.Main Outcome Measures Glare incidence,CS,and the influence of pupil diameter and P-IOL location to glare.Results None of the 104 P-IOL eyes occurred glare in day time.59 eyes (56.73%) complained of glare in dime condition.The glare incidence was 63.18% (14/22) in Phakic6 eyes,55.56% (30/54) in Verisyse eyes,and 53.27% (15/28) in PRL eyes (P=0.691).CS of all surgical eyes were improved in day time with or without glare luminous environment (P=0.000).And the CS of halo-complaining eyes was lower than of no halo eyes in night time with or without glare luminous environment (P=0.000).Glare oc- curred more frequently in eyes with the pupil diameter larger than P-IOL diameter in clime condition after surgery (10 in Phakic6 eyes, 20 in Verisyse eyes,12 in PRL eyes,all P

Clinical medical postgraduate education is an important part of higher education in China.We analyzed the current status,major problems existed,direction of the reform and the prospect in the training system.Unifying cultivation criterion,diversifying form of academic degree system,making selection standard strict for advisors and decreasing the proportion of research work for doctors were considered as the effective measures to address these issues.

Objective To observe the improvement in manifestation of port wine stains (PWS) with photodynamic therapy (PDT),and to further evaluate the safety and efficacy of PDT in treatment of PWS.Methods A total of 125 patients with PWS were treated with PDT.The lesion of PWS was exposured to the copper vapor laser locally after injection of photosensitizer PSD-007 based on the weight of body via vein.Before each session,lesions were photographed,and therapeutic effects were evaluated by physicians based on improvement in color,thickness,and lesion area recorded on the photos.Return visit was carried out after 2 months.The improvement rate was classified as 5 grades to value the therapeutic effect of last treatment.In addition,patients satisfaction was also investigated.The treatment was determined to terminate or continue depending on the change of red spot including color fading and flatting.Results Patients with PWS responsed remarkably to the PDT.The healing rate was 49.60 ％ and excellence rate 96.00 ％ after less than 4 session treatments.Among the total of healing patients,13.60 ％00 were released from PWS with no more than 2 courses of treat ment.The satisfactory rate of patients was 95.20 ％ during observation.Conclusions PDT is a safe and highly effective treatment for PWS with good cosmetic results in the dermatologic clinic.

To screen and identify the mimotopes for lipopolysaccharide(LPS) epitope, a rand om phage displayed dodecapeptide library was screened with a monoclonal antibody 2B4 spe ci fically against LPS conservative epitope. The positive clones were identified by phage ELISA and competitive inhibition assay by either S.typhi T 8-61 LPS or E.coli O111:B4 LPS. After three rounds of biopanning,the clones binding with 2B4 antibody were well enriched with positive rate of 80%. The bindings between 12 of positive phage clones and screening antibody were competitively inhibited by the two kinds of LPS,indicating that the positive clones have similar epitope with LPS. The positive peptide sequences were deduced from the corresponding DNA sequences. There were identical sequences among them. The seq uences were GPPQWFFSQPQL （5/12，41.7%），LPQYFWNTATTA （3/12，25%），FPQNHWNVP WAT（2/12，16.6%），HSQSFWNAPLAM and AHPWTHGYFPPL （1/12，8.3%） respectively . The results demonstrate that the peptides screened with 2B4 antibody are mimot opes for LPS conservative epitope.